OSTB_HUMAN
ID OSTB_HUMAN Reviewed; 128 AA.
AC Q86UW2; Q3SYF5;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Organic solute transporter subunit beta;
DE Short=OST-beta;
DE AltName: Full=Solute carrier family 51 subunit beta;
GN Name=SLC51B; Synonyms=OSTB;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Liver;
RX PubMed=12719432; DOI=10.1074/jbc.m301106200;
RA Seward D.J., Koh A.S., Boyer J.L., Ballatori N.;
RT "Functional complementation between a novel mammalian polygenic transport
RT complex and an evolutionarily ancient organic solute transporter, OSTalpha-
RT OSTbeta.";
RL J. Biol. Chem. 278:27473-27482(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND TRANSPORT ACTIVITY.
RX PubMed=16317684; DOI=10.1002/hep.20961;
RA Ballatori N., Christian W.V., Lee J.Y., Dawson P.A., Soroka C.J.,
RA Boyer J.L., Madejczyk M.S., Li N.;
RT "OSTalpha-OSTbeta: a major basolateral bile acid and steroid transporter in
RT human intestinal, renal, and biliary epithelia.";
RL Hepatology 42:1270-1279(2005).
RN [5]
RP INDUCTION BY NR1H4.
RX PubMed=16269519; DOI=10.1152/ajpgi.00430.2005;
RA Landrier J.-F., Eloranta J.J., Vavricka S.R., Kullak-Ublick G.A.;
RT "The nuclear receptor for bile acids, FXR, transactivates human organic
RT solute transporter-alpha and -beta genes.";
RL Am. J. Physiol. 290:G476-G485(2006).
RN [6]
RP INDUCTION BY NR1H4.
RX PubMed=16251721; DOI=10.1194/jlr.m500417-jlr200;
RA Lee H., Zhang Y., Lee F.Y., Nelson S.F., Gonzalez F.J., Edwards P.A.;
RT "FXR regulates organic solute transporters alpha and beta in the adrenal
RT gland, kidney, and intestine.";
RL J. Lipid Res. 47:201-214(2006).
RN [7]
RP INVOLVEMENT IN PBAM2.
RX PubMed=28898457; DOI=10.1002/hep.29516;
RA Sultan M., Rao A., Elpeleg O., Vaz F.M., Abu-Libdeh B., Karpen S.J.,
RA Dawson P.A.;
RT "Organic solute transporter-beta (SLC51B) deficiency in two brothers with
RT congenital diarrhea and features of cholestasis.";
RL Hepatology 68:590-598(2018).
CC -!- FUNCTION: Essential component of the Ost-alpha/Ost-beta complex, a
CC heterodimer that acts as the intestinal basolateral transporter
CC responsible for bile acid export from enterocytes into portal blood
CC (PubMed:16317684). Modulates SLC51A glycosylation, membrane trafficking
CC and stability activities (PubMed:16317684). The Ost-alpha/Ost-beta
CC complex efficiently transports the major species of bile acids
CC (taurocholate) (PubMed:16317684). Taurine conjugates are transported
CC more efficiently across the basolateral membrane than glycine-
CC conjugated bile acids (By similarity). Can also transport steroids such
CC as estrone 3-sulfate and dehydroepiandrosterone 3-sulfate, therefore
CC playing a role in the enterohepatic circulation of sterols
CC (PubMed:16317684). Able to transport eicosanoids such as prostaglandin
CC E2 (By similarity). {ECO:0000250|UniProtKB:Q8R000,
CC ECO:0000250|UniProtKB:Q90YM5, ECO:0000269|PubMed:16317684}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=taurocholate(out) = taurocholate(in); Xref=Rhea:RHEA:71703,
CC ChEBI:CHEBI:36257; Evidence={ECO:0000269|PubMed:16317684};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=estrone 3-sulfate(out) = estrone 3-sulfate(in);
CC Xref=Rhea:RHEA:71835, ChEBI:CHEBI:60050;
CC Evidence={ECO:0000269|PubMed:16317684};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dehydroepiandrosterone 3-sulfate(out) = dehydroepiandrosterone
CC 3-sulfate(in); Xref=Rhea:RHEA:71839, ChEBI:CHEBI:57905;
CC Evidence={ECO:0000269|PubMed:16317684};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tauroursodeoxycholate(out) = tauroursodeoxycholate(in);
CC Xref=Rhea:RHEA:71843, ChEBI:CHEBI:132028;
CC Evidence={ECO:0000250|UniProtKB:Q80WK2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycoursodeoxycholate(out) = glycoursodeoxycholate(in);
CC Xref=Rhea:RHEA:71847, ChEBI:CHEBI:132030;
CC Evidence={ECO:0000250|UniProtKB:Q80WK2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycocholate(out) = glycocholate(in); Xref=Rhea:RHEA:71851,
CC ChEBI:CHEBI:29746; Evidence={ECO:0000250|UniProtKB:Q80WK2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=taurochenodeoxycholate(out) = taurochenodeoxycholate(in);
CC Xref=Rhea:RHEA:71855, ChEBI:CHEBI:9407;
CC Evidence={ECO:0000250|UniProtKB:Q80WK2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycochenodeoxycholate(out) = glycochenodeoxycholate(in);
CC Xref=Rhea:RHEA:71859, ChEBI:CHEBI:36252;
CC Evidence={ECO:0000250|UniProtKB:Q80WK2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=taurodeoxycholate(out) = taurodeoxycholate(in);
CC Xref=Rhea:RHEA:71863, ChEBI:CHEBI:36261;
CC Evidence={ECO:0000250|UniProtKB:Q80WK2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycodeoxycholate(out) = glycodeoxycholate(in);
CC Xref=Rhea:RHEA:71867, ChEBI:CHEBI:82982;
CC Evidence={ECO:0000250|UniProtKB:Q80WK2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin E2(out) = prostaglandin E2(in);
CC Xref=Rhea:RHEA:50984, ChEBI:CHEBI:606564;
CC Evidence={ECO:0000250|UniProtKB:Q90YM5};
CC -!- SUBUNIT: Interacts with SLC51A. The Ost-alpha/Ost-beta complex is a
CC heterodimer composed of alpha (SLC51A) and beta (SLC51B) subunit;
CC induces the transport of SLC51A from the reticulum endoplasmic to the
CC plasma membrane (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q86UW2; Q12797-6: ASPH; NbExp=5; IntAct=EBI-12266756, EBI-12092171;
CC Q86UW2; Q9UHQ9: CYB5R1; NbExp=3; IntAct=EBI-12266756, EBI-953870;
CC Q86UW2; Q9HBU6: ETNK1; NbExp=3; IntAct=EBI-12266756, EBI-2834493;
CC Q86UW2; O43561-2: LAT; NbExp=5; IntAct=EBI-12266756, EBI-8070286;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12719432,
CC ECO:0000269|PubMed:16317684}; Single-pass membrane protein
CC {ECO:0000269|PubMed:12719432, ECO:0000269|PubMed:16317684}. Note=Mainly
CC restricted to the lateral and basal membranes of ileal enterocytes.
CC -!- TISSUE SPECIFICITY: Widely expressed with a high expression in ileum.
CC Expressed in testis, colon, liver, small intestine, kidney, ovary and
CC adrenal gland; and at low levels in heart, lung, brain, pituitary,
CC thyroid gland, uterus, prostate, mammary gland and fat.
CC {ECO:0000269|PubMed:12719432, ECO:0000269|PubMed:16317684}.
CC -!- INDUCTION: Positively regulated via NR1H4/FXR.
CC {ECO:0000269|PubMed:16251721, ECO:0000269|PubMed:16269519}.
CC -!- DOMAIN: The transmembrane domain (TM) is the major site of interaction
CC with SLC51A. The extracellular-membrane interface is absolutely
CC required for transport activity. The intracellular-membrane interface
CC is necessary for establishing the correct membrane orientation that is
CC essential for the heterodimer Ost-alpha/Ost-beta complex formation and
CC transport activity at the cell membrane surface (By similarity).
CC {ECO:0000250}.
CC -!- DISEASE: Bile acid malabsorption, primary, 2 (PBAM2) [MIM:619481]: An
CC autosomal recessive disorder characterized by chronic diarrhea, severe
CC fat-soluble vitamin deficiency, and features of cholestatic liver
CC disease. {ECO:0000269|PubMed:28898457}. Note=The disease may be caused
CC by variants affecting the gene represented in this entry.
CC -!- SIMILARITY: Belongs to the OST-beta family. {ECO:0000305}.
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DR EMBL; AY194242; AAP23992.1; -; mRNA.
DR EMBL; CH471082; EAW77708.1; -; Genomic_DNA.
DR EMBL; BC103842; AAI03843.1; -; mRNA.
DR EMBL; BC103843; AAI03844.1; -; mRNA.
DR EMBL; BC103844; AAI03845.1; -; mRNA.
DR CCDS; CCDS10199.1; -.
DR RefSeq; NP_849190.2; NM_178859.3.
DR RefSeq; XP_005254216.1; XM_005254159.4.
DR AlphaFoldDB; Q86UW2; -.
DR SMR; Q86UW2; -.
DR BioGRID; 125821; 31.
DR IntAct; Q86UW2; 5.
DR STRING; 9606.ENSP00000335292; -.
DR DrugBank; DB00286; Conjugated estrogens.
DR DrugBank; DB00390; Digoxin.
DR DrugBank; DB00917; Dinoprostone.
DR DrugBank; DB04348; Taurocholic acid.
DR TCDB; 2.A.82.1.2; the organic solute transporter (ost) family.
DR iPTMnet; Q86UW2; -.
DR PhosphoSitePlus; Q86UW2; -.
DR BioMuta; SLC51B; -.
DR DMDM; 187471105; -.
DR jPOST; Q86UW2; -.
DR MassIVE; Q86UW2; -.
DR PaxDb; Q86UW2; -.
DR PeptideAtlas; Q86UW2; -.
DR PRIDE; Q86UW2; -.
DR Antibodypedia; 1475; 69 antibodies from 12 providers.
DR DNASU; 123264; -.
DR Ensembl; ENST00000334287.3; ENSP00000335292.2; ENSG00000186198.4.
DR GeneID; 123264; -.
DR KEGG; hsa:123264; -.
DR MANE-Select; ENST00000334287.3; ENSP00000335292.2; NM_178859.4; NP_849190.2.
DR UCSC; uc002aog.3; human.
DR CTD; 123264; -.
DR DisGeNET; 123264; -.
DR GeneCards; SLC51B; -.
DR HGNC; HGNC:29956; SLC51B.
DR HPA; ENSG00000186198; Tissue enhanced (cervix, intestine).
DR MIM; 612085; gene.
DR MIM; 619481; phenotype.
DR neXtProt; NX_Q86UW2; -.
DR OpenTargets; ENSG00000186198; -.
DR VEuPathDB; HostDB:ENSG00000186198; -.
DR eggNOG; ENOG502S380; Eukaryota.
DR GeneTree; ENSGT00390000010409; -.
DR HOGENOM; CLU_158049_0_0_1; -.
DR InParanoid; Q86UW2; -.
DR OMA; EMLWVFR; -.
DR PhylomeDB; Q86UW2; -.
DR PathwayCommons; Q86UW2; -.
DR Reactome; R-HSA-159418; Recycling of bile acids and salts.
DR SignaLink; Q86UW2; -.
DR BioGRID-ORCS; 123264; 13 hits in 1060 CRISPR screens.
DR ChiTaRS; SLC51B; human.
DR GeneWiki; OSTbeta; -.
DR GenomeRNAi; 123264; -.
DR Pharos; Q86UW2; Tbio.
DR PRO; PR:Q86UW2; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q86UW2; protein.
DR Bgee; ENSG00000186198; Expressed in ileal mucosa and 108 other tissues.
DR Genevisible; Q86UW2; HS.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0015125; F:bile acid transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR GO; GO:0015721; P:bile acid and bile salt transport; ISS:UniProtKB.
DR GO; GO:0032782; P:bile acid secretion; IPI:UniProtKB.
DR GO; GO:0070863; P:positive regulation of protein exit from endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0060050; P:positive regulation of protein glycosylation; ISS:UniProtKB.
DR GO; GO:0090314; P:positive regulation of protein targeting to membrane; ISS:UniProtKB.
DR GO; GO:0031647; P:regulation of protein stability; ISS:UniProtKB.
DR InterPro; IPR029387; OSTbeta.
DR Pfam; PF15048; OSTbeta; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Lipid transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..128
FT /note="Organic solute transporter subunit beta"
FT /id="PRO_0000331554"
FT TOPO_DOM 1..35
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 66
FT /note="K -> T (in Ref. 1; AAP23992)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 128 AA; 14346 MW; A6D6F9391CB15794 CRC64;
MEHSEGAPGD PAGTVVPQEL LEEMLWFFRV EDASPWNHSI LALAAVVVII SMVLLGRSIQ
ASRKEKMQPP EKETPEVLHL DEAKDHNSLN NLRETLLSEK PNLAQVELEL KERDVLSVFL
PDVPETES
