OSW5_YEAST
ID OSW5_YEAST Reviewed; 148 AA.
AC P40219; D6VZX0;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Outer spore wall protein 5;
GN Name=OSW5; OrderedLocusNames=YMR148W; ORFNames=YM9375.18;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP FUNCTION.
RX PubMed=19779569; DOI=10.1371/journal.pone.0007184;
RA Suda Y., Rodriguez R.K., Coluccio A.E., Neiman A.M.;
RT "A screen for spore wall permeability mutants identifies a secreted
RT protease required for proper spore wall assembly.";
RL PLoS ONE 4:E7184-E7184(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Involved in spore wall assembly.
CC {ECO:0000269|PubMed:19779569}.
CC -!- INTERACTION:
CC P40219; P53756: PDR18; NbExp=2; IntAct=EBI-3681488, EBI-28581;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- MISCELLANEOUS: Present with 922 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the OSW5 family. {ECO:0000305}.
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DR EMBL; Z47071; CAA87363.1; -; Genomic_DNA.
DR EMBL; AY558413; AAS56739.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10044.1; -; Genomic_DNA.
DR PIR; S50405; S50405.
DR RefSeq; NP_013868.1; NM_001182650.1.
DR AlphaFoldDB; P40219; -.
DR BioGRID; 35324; 86.
DR IntAct; P40219; 1.
DR MINT; P40219; -.
DR STRING; 4932.YMR148W; -.
DR iPTMnet; P40219; -.
DR MaxQB; P40219; -.
DR PaxDb; P40219; -.
DR PRIDE; P40219; -.
DR EnsemblFungi; YMR148W_mRNA; YMR148W; YMR148W.
DR GeneID; 855179; -.
DR KEGG; sce:YMR148W; -.
DR SGD; S000004756; OSW5.
DR VEuPathDB; FungiDB:YMR148W; -.
DR eggNOG; ENOG502SA41; Eukaryota.
DR HOGENOM; CLU_147574_0_0_1; -.
DR InParanoid; P40219; -.
DR OMA; SLETXAN; -.
DR BioCyc; YEAST:G3O-32840-MON; -.
DR ChiTaRS; OSW5; yeast.
DR PRO; PR:P40219; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P40219; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030476; P:ascospore wall assembly; IMP:SGD.
DR InterPro; IPR031430; Osw5.
DR Pfam; PF17062; Osw5; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Sporulation; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..148
FT /note="Outer spore wall protein 5"
FT /id="PRO_0000203308"
FT TOPO_DOM 1..7
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 83..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..109
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 102
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 148 AA; 16384 MW; C533FD19B2D1316A CRC64;
MVSTATFFFF VYLTLFVVIG FFSSLFIIPL LGISFVFAIG VVSFGFCSNM SFKMAQLIYV
RADAFLKKVL DKMALQTQPA QLQEPQEPLS TLRPVSNPTI PSPLRQTARP SKFVTEEDVI
FEPVSAQSAI ARSLETTANK AGNKFQLS
