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OTAA_ASPC5
ID   OTAA_ASPC5              Reviewed;        2541 AA.
AC   A0A1R3RGK0;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2017, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=Highly reducing polyketide synthase otaA {ECO:0000303|PubMed:30054361};
DE            EC=2.3.1.- {ECO:0000269|PubMed:24699234};
DE   AltName: Full=Ochratoxin A biosynthesis cluster protein A {ECO:0000303|PubMed:30054361};
GN   Name=otaA {ECO:0000303|PubMed:30054361};
GN   Synonyms=OTApks {ECO:0000303|PubMed:24699234}; ORFNames=ASPCADRAFT_173482;
OS   Aspergillus carbonarius (strain ITEM 5010).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=602072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ITEM 5010;
RX   PubMed=28196534; DOI=10.1186/s13059-017-1151-0;
RA   de Vries R.P., Riley R., Wiebenga A., Aguilar-Osorio G., Amillis S.,
RA   Uchima C.A., Anderluh G., Asadollahi M., Askin M., Barry K., Battaglia E.,
RA   Bayram O., Benocci T., Braus-Stromeyer S.A., Caldana C., Canovas D.,
RA   Cerqueira G.C., Chen F., Chen W., Choi C., Clum A., Dos Santos R.A.,
RA   Damasio A.R., Diallinas G., Emri T., Fekete E., Flipphi M., Freyberg S.,
RA   Gallo A., Gournas C., Habgood R., Hainaut M., Harispe M.L., Henrissat B.,
RA   Hilden K.S., Hope R., Hossain A., Karabika E., Karaffa L., Karanyi Z.,
RA   Krasevec N., Kuo A., Kusch H., LaButti K., Lagendijk E.L., Lapidus A.,
RA   Levasseur A., Lindquist E., Lipzen A., Logrieco A.F., MacCabe A.,
RA   Maekelae M.R., Malavazi I., Melin P., Meyer V., Mielnichuk N., Miskei M.,
RA   Molnar A.P., Mule G., Ngan C.Y., Orejas M., Orosz E., Ouedraogo J.P.,
RA   Overkamp K.M., Park H.-S., Perrone G., Piumi F., Punt P.J., Ram A.F.,
RA   Ramon A., Rauscher S., Record E., Riano-Pachon D.M., Robert V., Roehrig J.,
RA   Ruller R., Salamov A., Salih N.S., Samson R.A., Sandor E., Sanguinetti M.,
RA   Schuetze T., Sepcic K., Shelest E., Sherlock G., Sophianopoulou V.,
RA   Squina F.M., Sun H., Susca A., Todd R.B., Tsang A., Unkles S.E.,
RA   van de Wiele N., van Rossen-Uffink D., Oliveira J.V., Vesth T.C.,
RA   Visser J., Yu J.-H., Zhou M., Andersen M.R., Archer D.B., Baker S.E.,
RA   Benoit I., Brakhage A.A., Braus G.H., Fischer R., Frisvad J.C.,
RA   Goldman G.H., Houbraken J., Oakley B., Pocsi I., Scazzocchio C.,
RA   Seiboth B., vanKuyk P.A., Wortman J., Dyer P.S., Grigoriev I.V.;
RT   "Comparative genomics reveals high biological diversity and specific
RT   adaptations in the industrially and medically important fungal genus
RT   Aspergillus.";
RL   Genome Biol. 18:RESEARCH28.1-RESEARCH28.45(2017).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, DOMAIN, AND PATHWAY.
RX   PubMed=24699234; DOI=10.1016/j.ijfoodmicro.2014.03.013;
RA   Gallo A., Knox B.P., Bruno K.S., Solfrizzo M., Baker S.E., Perrone G.;
RT   "Identification and characterization of the polyketide synthase involved in
RT   ochratoxin A biosynthesis in Aspergillus carbonarius.";
RL   Int. J. Food Microbiol. 179:10-17(2014).
RN   [3]
RP   INDUCTION.
RX   PubMed=27548221; DOI=10.3390/toxins8080242;
RA   El Khoury R., Atoui A., Verheecke C., Maroun R., El Khoury A., Mathieu F.;
RT   "Essential oils modulate gene expression and ochratoxin A production in
RT   Aspergillus carbonarius.";
RL   Toxins 8:0-0(2016).
RN   [4]
RP   FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=30054361; DOI=10.1128/aem.01009-18;
RA   Wang Y., Wang L., Wu F., Liu F., Wang Q., Zhang X., Selvaraj J.N., Zhao Y.,
RA   Xing F., Yin W.B., Liu Y.;
RT   "A consensus ochratoxin A biosynthetic pathway: insights from the genome
RT   sequence of Aspergillus ochraceus and a comparative genomic analysis.";
RL   Appl. Environ. Microbiol. 84:0-0(2018).
RN   [5]
RP   FUNCTION.
RX   PubMed=33391201; DOI=10.3389/fmicb.2020.581309;
RA   Ferrara M., Gallo A., Perrone G., Magista D., Baker S.E.;
RT   "Comparative genomic analysis of ochratoxin A biosynthetic cluster in
RT   producing fungi: new evidence of a cyclase gene involvement.";
RL   Front. Microbiol. 11:581309-581309(2020).
RN   [6]
RP   INDUCTION.
RX   PubMed=33540740; DOI=10.3390/toxins13020111;
RA   Gerin D., Garrapa F., Ballester A.R., Gonzalez-Candelas L.,
RA   De Miccolis Angelini R.M., Faretra F., Pollastro S.;
RT   "Functional role of Aspergillus carbonarius AcOTAbZIP gene, a bZIP
RT   transcription factor within the OTA gene cluster.";
RL   Toxins 13:0-0(2021).
CC   -!- FUNCTION: Highly reducing polyketide synthase; part of the gene cluster
CC       that mediates the biosynthesis of ochratoxin A (OTA), a mycotoxin
CC       composed of a chlorinated type I polyketide dihydroisocoumarin moiety
CC       linked to L-phenylalanine, and demonstrated to have nephrotoxic,
CC       immunotoxic, genotoxic, neurotoxic, and teratogenic properties
CC       (PubMed:24699234, PubMed:30054361). OtaA catalyzes the condensation of
CC       one acetate and 4 malonate units to form the isocoumarin group
CC       (PubMed:24699234). The pathway begins with the highly reducing
CC       polyketide synthase otaA that catalyzes the formation of the
CC       isocoumarin group during the initial stages of biosynthesis, starting
CC       from one acetate and 4 malonate units, to originate the characteristic
CC       pentaketide skeleton 7-methylmellein (7-MM) of the OTA molecule. The
CC       newly identified cyclase otaY might be involved in the polyketide
CC       cyclization reaction during the initial steps of the OTA biosynthesis.
CC       7-MM is then oxidized into 7-carboxymellein (also called ochratoxin
CC       beta) by the cytochrome P450 monooxygenase otaC. The NRPS encoded by
CC       the otaB gene is involved in the linking of phenylalanine to the
CC       dihydroisocoumarin ring. The reaction catalyzed by NRPS results in the
CC       production of ochratoxin B (OTB), which is the non-chlorinated analog
CC       of OTA and which subsequently serves as the substrate of the halogenase
CC       otaD for chlorination activity to form the final molecular structure of
CC       OTA, containing a chlorine atom in the C-5 position of the molecule
CC       (PubMed:33391201) (Probable). {ECO:0000269|PubMed:24699234,
CC       ECO:0000269|PubMed:30054361, ECO:0000305|PubMed:33391201}.
CC   -!- COFACTOR:
CC       Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC         Evidence={ECO:0000255};
CC       Note=Binds 1 phosphopantetheine covalently. {ECO:0000255};
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:24699234,
CC       ECO:0000269|PubMed:30054361}.
CC   -!- INDUCTION: Expression is down-regulated in the presence of fennel,
CC       cardamom, chamomile, celery, anise and rosemary essential oils
CC       (PubMed:27548221). Expression is positively regulated by the cluster-
CC       specific transcription factor otaR1 (PubMed:30054361, PubMed:33540740).
CC       Expression is also modulated by a second regulator, otaR2, which is
CC       adjacent to the biosynthetic gene cluster (PubMed:30054361).
CC       {ECO:0000269|PubMed:27548221, ECO:0000269|PubMed:30054361,
CC       ECO:0000269|PubMed:33540740}.
CC   -!- DOMAIN: Multidomain protein; including a ketosynthase (KS) that
CC       catalyzes repeated decarboxylative condensation to elongate the
CC       polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects
CC       and transfers the extender unit malonyl-CoA; a dehydratase (DH) domain
CC       that reduces hydroxyl groups to enoyl groups; a methyltransferase
CC       (CMeT) domain responsible for the incorporation of methyl groups; an
CC       enoylreductase (ER) domain that reduces enoyl groups to alkyl group; a
CC       ketoreductase (KR) domain that catalyzes beta-ketoreduction steps; and
CC       an acyl-carrier protein (ACP) that serves as the tether of the growing
CC       and completed polyketide via its phosphopantetheinyl arm.
CC       {ECO:0000305|PubMed:24699234}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes the production of ochratoxin A and its
CC       degradation derivative ochratoxin alpha. {ECO:0000269|PubMed:24699234,
CC       ECO:0000269|PubMed:30054361}.
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DR   EMBL; KV907504; OOF93599.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1R3RGK0; -.
DR   SMR; A0A1R3RGK0; -.
DR   STRING; 602072.A0A1R3RGK0; -.
DR   EnsemblFungi; OOF93599; OOF93599; ASPCADRAFT_173482.
DR   VEuPathDB; FungiDB:ASPCADRAFT_173482; -.
DR   OMA; KMRGGEF; -.
DR   OrthoDB; 19161at2759; -.
DR   BioCyc; MetaCyc:MON-21059; -.
DR   Proteomes; UP000188318; Unassembled WGS sequence.
DR   GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:1900818; P:ochratoxin A biosynthetic process; IMP:GO_Central.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   Gene3D; 3.10.129.110; -; 1.
DR   Gene3D; 3.40.366.10; -; 1.
DR   Gene3D; 3.40.47.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR001227; Ac_transferase_dom_sf.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR013154; ADH_N.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR013217; Methyltransf_12.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR032821; PKS_assoc.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR042104; PKS_dehydratase_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR016039; Thiolase-like.
DR   Pfam; PF00698; Acyl_transf_1; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF16197; KAsynt_C_assoc; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   Pfam; PF08659; KR; 1.
DR   Pfam; PF08242; Methyltransf_12; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   Pfam; PF14765; PS-DH; 1.
DR   SMART; SM00827; PKS_AT; 1.
DR   SMART; SM00826; PKS_DH; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SMART; SM00825; PKS_KS; 1.
DR   SMART; SM00823; PKS_PP; 1.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 2.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF53901; SSF53901; 1.
DR   SUPFAM; SSF55048; SSF55048; 1.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR   PROSITE; PS50075; CARRIER; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Methyltransferase; Multifunctional enzyme; NADP;
KW   Oxidoreductase; Phosphopantetheine; Phosphoprotein; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..2541
FT                   /note="Highly reducing polyketide synthase otaA"
FT                   /id="PRO_0000440588"
FT   DOMAIN          2453..2530
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   REGION          12..434
FT                   /note="Ketosynthase (KS) domain"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:24699234"
FT   REGION          571..888
FT                   /note="Malonyl-CoA:ACP transacylase (MAT) domain"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:24699234"
FT   REGION          957..1250
FT                   /note="Dehydrogenase (DH) domain"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:24699234"
FT   REGION          1433..1605
FT                   /note="Methyltransferase (CMeT) domain"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:24699234"
FT   REGION          1857..1919
FT                   /note="Enoyl reductase (ER) (ER) domain"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:24699234"
FT   REGION          2165..2344
FT                   /note="Ketoreductase (KR) domain"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:24699234"
FT   ACT_SITE        182
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT   BINDING         1420
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01019"
FT   BINDING         1442
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01019"
FT   MOD_RES         2490
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ   SEQUENCE   2541 AA;  274798 MW;  F2ACF09BA03616CF CRC64;
     MTFTSHPQSE PLAIIGLACK YANDINSPLD LYQQVMAARS MHGPMPPSRM DAAFYYHPSS
     EATGTTYAKG GYFLQSDLNA FDSPFFQLSE IDVLAMDPQQ KMLLENVYHA LENAGIPLKD
     AVSSSTSVFV GCSNNDHLAL ANADLLLALK GKGTGTSPSI LANRISWFYD FQGTSQTIDT
     ACSSSLVAFH QGCMDVRAGK STMSIISGVN LMEHPAPTMY LSSLGVLSPD GRSMSFDARA
     NGYGRGEGLG TVIIKPLTAA LRDGNRIRAI VRSTGSNQDG RTPGITVPSP TAQERLIREV
     YKAADLDPSR TGYVEAHGTG TPVGDPLEVQ AISAALGMSR DSPLYVGSVK SVVGHLEGGA
     GMAGLISATM AVESKTIPPV AGLQTLNPRI PQRPDLKFAK EATPWPREDV RRASINSFGF
     GGTNAHVVLE DVEGFFSDLF GQQLPGALQL SEVTSKALVP SAMKSAVNGI PADQPPKESS
     VNRLFVISAF DEAGIQRNAA SLASHLESMR AITGSDGEER LLNDLCHTLN EKRTRFDWRS
     YHVADSIDSL RNSLQNPRPI RQSPAEKVVR FIFTGQGANW AGMAYDLLVY PLFRRRIQEA
     AIFLKELGSD WDLYERIASQ SGELDEPTFA QSSCVAVQVA LVDLLASWNV TPQTVVGHSS
     GEIAAAYCAG QISRQAAWKV AFCRGQVCAR RTDGQGRMLA AAMPVTQLEQ LVARVNKGQS
     TAVKVGCYNS PKNLTLTGRA EDILRAKLEL DDVGALNRLL PVKVAYHSDY MRDAAPEYLD
     LLGDLDFGDS IHADAGIKMV SSVTGRAVSA GEAQQPSYWV DNLVSPVRFS TALLASMDDP
     SATGAREDAL IEIGPHSTLR TAIKETFADV REFQSIQYGS LLKRYETDGS TILRTFGMLV
     CSGHKISLAA INDRRVGAKK TPRLLTGLPS YAFDHSRSMR GTSRRIEQAK FPAYKRHELL
     GVPVEDTNPV EQRWRNILRP DDLPWLRMNR MNGQIHFPGV AYLLMATEAA IQRVGNTVAI
     SGVRLGNVSM LAPLPIPDSA AGVEIQFSIY PMKIHANSGT DWSTFRIVSY DSAEKTWTEH
     CVGSVRVETG PHESHEPHPG NATREECTES VDIAQMYSRF TTAGMDFGEY LRNIQEMKLS
     PDHQACTATI TAPDIPCQAH DHYSLHPCTF ESILHALLHL CKSSQGPMVT TYIEEVLVLS
     PQDTGVCGFE ACAQTQRASA TTWRSDVTIT ANTGRQQIRV TGLDLVQLPP SEDASDAESF
     YVVKWKPDVK LLTSVDALRD SASMYVAQHL PTLDEHEGFQ LASGIFLLDT MDYVTRTGLP
     ALPQHHQAFM QWMEKECRSI ADGTVPLLDT ALFEGIRASP DRRRELLARV AQLSARGELL
     VRVGTQMVPI LEQKIDCLEV MFGPDNLMDR TYEEGLPGQI APSVAGYLHC LAHAQTGIKV
     LEVGAGTGSA TKVILDSLKP TERQDGGGLV SSVSTYHFTD ISAAFFEKAR ARFPDWADIL
     RPKVLNIELD PADQGFEMGS YDLVIATHVL HATADLSVSL KNIRGLLKEG GDLIVIENIQ
     PDLMCSPLAF GLLPGWWRSV EPYRKTNPLI TKDQWDQELR NAGLQSRLLI DDTDEGVNEM
     TAFVASRVRE PPATQHVCSI IYSSRYGGQY ELASQVARDL PPSCTASLVD LADISPEHTS
     TIGIVLVGYQ GLDLSELSAH EYDRVNFLLT AFHRLLWVTC DEDEVPKSAM ASGLVRTARW
     ERDHDGVNFI LLGISHRVPS ASAAVSQMIR VCDHAFFSHE LVPRNAEFRL EGSVLLTNRL
     FPATGINECI ASSSRPRSKQ VALEAVQHPV KLTSIGPHQP NGFHFVEDPQ VDEPLLPDEV
     KIQIRAVGLD ESDVEEMNRL IPGESAGSQG TGVVVEVGPA VHDIHVGDRV MALRTGHSGS
     LQTVLRTHSS AVTQVPEGLS LADAAAVPLP FTTAYHGLVN VARLEPQDTI LIHNAGGATG
     QAAVQFACML GATVYATVES DAQRQALLDY GVDRSRLLDG PSFAQQLARR GAKGSVDVLF
     NLSRESLEDR DLACLSQFGR LVGVHGQGSL PAGPTNRSYA TVSIRELVQV RPKALHGTLR
     TISDLLTSRA IRPITPVRAG YSELQTVLSQ IRQGNAGPWV LEPRANDTIP VAMKPLGDYQ
     FDPCASYLLI GGFGGIGRSV VRWMLTRGAK NFIFLSRSGA SSVPAKQLCA DLLDAGCGVS
     DTVCDVTDAT AVENALQQCG KSMPPIRGCL QCSMVLEDSM LSNMSHAQFL NAITPKVQGT
     IHVASALSSV KSNLDFFVLL SSSAGIIGNR GQANYSAANA FLDAFAAHLV SRGYPATSIS
     LGSVLSVGWV AENQDRLPIA LSYGAISEDL LLAILEYHMD PSWGAAQSPG TCHTVAGVRS
     ARDFQRQSIP LPGFMAYPLF SPLRAIAGAS QTAEEVAEAP IAQGLRGATS MEDAVELVTR
     AIVYKLARIM ALSAKEIDAQ RSLASYGVDS LVTVDLKAWF QREVGATVAS GDLLGDSTIV
     QLAQQAAGGS RLVSVAMKGT E
 
 
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