OTAR1_ASPC5
ID OTAR1_ASPC5 Reviewed; 247 AA.
AC A0A1R3RGK4;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 25-MAY-2022, entry version 15.
DE RecName: Full=Transcription factor otaR1 {ECO:0000303|PubMed:30054361};
DE AltName: Full=Ochratoxin A biosynthesis cluster protein R1 {ECO:0000303|PubMed:30054361};
GN Name=otaR1 {ECO:0000303|PubMed:30054361};
GN Synonyms=OTAbZIP {ECO:0000303|PubMed:33540740}; ORFNames=ASPCADRAFT_7821;
OS Aspergillus carbonarius (strain ITEM 5010).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=602072;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ITEM 5010;
RX PubMed=28196534; DOI=10.1186/s13059-017-1151-0;
RA de Vries R.P., Riley R., Wiebenga A., Aguilar-Osorio G., Amillis S.,
RA Uchima C.A., Anderluh G., Asadollahi M., Askin M., Barry K., Battaglia E.,
RA Bayram O., Benocci T., Braus-Stromeyer S.A., Caldana C., Canovas D.,
RA Cerqueira G.C., Chen F., Chen W., Choi C., Clum A., Dos Santos R.A.,
RA Damasio A.R., Diallinas G., Emri T., Fekete E., Flipphi M., Freyberg S.,
RA Gallo A., Gournas C., Habgood R., Hainaut M., Harispe M.L., Henrissat B.,
RA Hilden K.S., Hope R., Hossain A., Karabika E., Karaffa L., Karanyi Z.,
RA Krasevec N., Kuo A., Kusch H., LaButti K., Lagendijk E.L., Lapidus A.,
RA Levasseur A., Lindquist E., Lipzen A., Logrieco A.F., MacCabe A.,
RA Maekelae M.R., Malavazi I., Melin P., Meyer V., Mielnichuk N., Miskei M.,
RA Molnar A.P., Mule G., Ngan C.Y., Orejas M., Orosz E., Ouedraogo J.P.,
RA Overkamp K.M., Park H.-S., Perrone G., Piumi F., Punt P.J., Ram A.F.,
RA Ramon A., Rauscher S., Record E., Riano-Pachon D.M., Robert V., Roehrig J.,
RA Ruller R., Salamov A., Salih N.S., Samson R.A., Sandor E., Sanguinetti M.,
RA Schuetze T., Sepcic K., Shelest E., Sherlock G., Sophianopoulou V.,
RA Squina F.M., Sun H., Susca A., Todd R.B., Tsang A., Unkles S.E.,
RA van de Wiele N., van Rossen-Uffink D., Oliveira J.V., Vesth T.C.,
RA Visser J., Yu J.-H., Zhou M., Andersen M.R., Archer D.B., Baker S.E.,
RA Benoit I., Brakhage A.A., Braus G.H., Fischer R., Frisvad J.C.,
RA Goldman G.H., Houbraken J., Oakley B., Pocsi I., Scazzocchio C.,
RA Seiboth B., vanKuyk P.A., Wortman J., Dyer P.S., Grigoriev I.V.;
RT "Comparative genomics reveals high biological diversity and specific
RT adaptations in the industrially and medically important fungal genus
RT Aspergillus.";
RL Genome Biol. 18:RESEARCH28.1-RESEARCH28.45(2017).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=30054361; DOI=10.1128/aem.01009-18;
RA Wang Y., Wang L., Wu F., Liu F., Wang Q., Zhang X., Selvaraj J.N., Zhao Y.,
RA Xing F., Yin W.B., Liu Y.;
RT "A consensus ochratoxin A biosynthetic pathway: insights from the genome
RT sequence of Aspergillus ochraceus and a comparative genomic analysis.";
RL Appl. Environ. Microbiol. 84:0-0(2018).
RN [3]
RP FUNCTION.
RX PubMed=33391201; DOI=10.3389/fmicb.2020.581309;
RA Ferrara M., Gallo A., Perrone G., Magista D., Baker S.E.;
RT "Comparative genomic analysis of ochratoxin A biosynthetic cluster in
RT producing fungi: new evidence of a cyclase gene involvement.";
RL Front. Microbiol. 11:581309-581309(2020).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=33540740; DOI=10.3390/toxins13020111;
RA Gerin D., Garrapa F., Ballester A.R., Gonzalez-Candelas L.,
RA De Miccolis Angelini R.M., Faretra F., Pollastro S.;
RT "Functional role of Aspergillus carbonarius AcOTAbZIP gene, a bZIP
RT transcription factor within the OTA gene cluster.";
RL Toxins 13:0-0(2021).
CC -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC the biosynthesis of ochratoxin A (OTA), a mycotoxin demonstrated to
CC have nephrotoxic, immunotoxic, genotoxic, neurotoxic, and teratogenic
CC properties (PubMed:30054361, PubMed:33540740). Positively regulates the
CC expression of the cluster genes otaA, otaB, otaC and otaD, and the
CC subsequent production of OTA (PubMed:30054361, PubMed:33540740).
CC {ECO:0000269|PubMed:30054361, ECO:0000269|PubMed:33540740}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978}.
CC -!- DISRUPTION PHENOTYPE: Results in the loss of production of ochratoxin A
CC and the intermediates ochratoxin B and ochratoxin beta
CC (PubMed:33540740, PubMed:30054361). Leads to down-regulation of
CC expression of the cluster genes otaA, otaB, otaC and otaD
CC (PubMed:30054361, PubMed:33540740). {ECO:0000269|PubMed:30054361,
CC ECO:0000269|PubMed:33540740}.
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DR EMBL; KV907504; OOF93603.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1R3RGK4; -.
DR SMR; A0A1R3RGK4; -.
DR EnsemblFungi; OOF93603; OOF93603; ASPCADRAFT_7821.
DR VEuPathDB; FungiDB:ASPCADRAFT_7821; -.
DR OrthoDB; 49852at5052; -.
DR Proteomes; UP000188318; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:1900818; P:ochratoxin A biosynthetic process; IMP:GO_Central.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
PE 3: Inferred from homology;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..247
FT /note="Transcription factor otaR1"
FT /id="PRO_0000440592"
FT DOMAIN 156..219
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 156..196
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 198..212
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 18..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 247 AA; 27334 MW; 280C5ABD34463A5C CRC64;
MEPALISPEL LDGSSLLPHS SADESSSTGL SLGSLLSSSN DLSSETSMPQ FEQLERMLGS
PTIANHVDFT TQSTDLGFLS DPNITLSSIE LTELFSIPSD NASLMPEPSR QPSPLKAPDL
QTTPPATGLI DSEANPTSVP LGNSVEQSAD VKQLRKKYHE KYKERNRVAA GKSRQKQVDL
IELLQAEQRE EERRRKALER ELSQIHKELL DLKQELQHHI RIANCMTMMS HGAHMQTLGL
LAQDMLR
