OTAR1_ASPNC
ID OTAR1_ASPNC Reviewed; 221 AA.
AC A2R6G8;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Transcription factor otaR1 {ECO:0000303|PubMed:27667988};
DE AltName: Full=Ochratoxin biosynthesis cluster bZIP transcription factor {ECO:0000303|PubMed:35143724};
DE AltName: Full=Ochratoxin biosynthesis cluster protein 4 {ECO:0000303|PubMed:27667988};
DE AltName: Full=Ochratoxin biosynthesis cluster protein R1 {ECO:0000303|PubMed:33391201};
GN Name=otaR1 {ECO:0000303|PubMed:33391201};
GN Synonyms=ota4 {ECO:0000303|PubMed:27667988},
GN OTAbZIP {ECO:0000303|PubMed:35143724}; ORFNames=An15g07890;
OS Aspergillus niger (strain CBS 513.88 / FGSC A1513).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=425011;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 513.88 / FGSC A1513 / ATCC MYA-4892;
RX PubMed=17259976; DOI=10.1038/nbt1282;
RA Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J.,
RA Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R.,
RA Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X.,
RA Contreras R., Cornell M., Coutinho P.M., Danchin E.G.J., Debets A.J.M.,
RA Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M.,
RA d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J.,
RA Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W.,
RA van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M.,
RA Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X.,
RA van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A.,
RA Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E.,
RA Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J.,
RA Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H.,
RA Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.;
RT "Genome sequencing and analysis of the versatile cell factory Aspergillus
RT niger CBS 513.88.";
RL Nat. Biotechnol. 25:221-231(2007).
RN [2]
RP FUNCTION.
RX PubMed=27667988; DOI=10.3389/fmicb.2016.01412;
RA Susca A., Proctor R.H., Morelli M., Haidukowski M., Gallo A.,
RA Logrieco A.F., Moretti A.;
RT "Variation in fumonisin and ochratoxin production associated with
RT differences in biosynthetic gene content in Aspergillus niger and A.
RT welwitschiae isolates from multiple crop and geographic origins.";
RL Front. Microbiol. 7:1412-1412(2016).
RN [3]
RP NOMENCLATURE, AND FUNCTION.
RX PubMed=33391201; DOI=10.3389/fmicb.2020.581309;
RA Ferrara M., Gallo A., Perrone G., Magista D., Baker S.E.;
RT "Comparative genomic analysis of ochratoxin A biosynthetic cluster in
RT producing fungi: new evidence of a cyclase gene involvement.";
RL Front. Microbiol. 11:581309-581309(2020).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=35143724; DOI=10.1021/acs.jafc.1c08160;
RA Zhang J., Li L., Yang Y., Zhao C., Hu J., Xue X., Gao Q., Wang D.,
RA Zhuang Z., Zhang Y.;
RT "Deletion and overexpression of the AnOTAbzip gene, a positive regulator of
RT ochratoxin A biosynthesis in Aspergillus niger.";
RL J. Agric. Food Chem. 70:2169-2178(2022).
CC -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC the biosynthesis of ochratoxin A (OTA), a mycotoxin demonstrated to
CC have nephrotoxic, immunotoxic, genotoxic, neurotoxic, and teratogenic
CC properties (PubMed:27667988, PubMed:33391201). Positively regulates the
CC expression of the OTA biosynthetic genes and subsequent production of
CC OTA (PubMed:35143724). Probably binds to conserved 5'-ACGT-3' bZIP
CC binding motifs found in multiple copies (3 to 4) in the promoters of
CC the OTA biosynthetic genes (PubMed:35143724). Acts not only as a
CC pathway-specific regulator of the OTA cluster but binds also at other
CC chromosomal positions outside the OTA cluster and can act as a broad
CC regulator (PubMed:35143724). Negatively regulates pathogenicity and
CC plays a critical role in tolerance to reactive oxygen species (ROS)
CC (PubMed:35143724). {ECO:0000269|PubMed:27667988,
CC ECO:0000269|PubMed:33391201, ECO:0000269|PubMed:35143724}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978}.
CC -!- DISRUPTION PHENOTYPE: Leads to the loss of ochratoxin A (OTA)
CC production and the down-regulation of the OTA biosynthetic genes
CC (PubMed:35143724). Produces greater lesions on grape berries and is
CC more tolerant to oxidative stress (PubMed:35143724).
CC {ECO:0000269|PubMed:35143724}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AM270352; CAK42676.1; -; Genomic_DNA.
DR AlphaFoldDB; A2R6G8; -.
DR SMR; A2R6G8; -.
DR PaxDb; A2R6G8; -.
DR EnsemblFungi; CAK42676; CAK42676; An15g07890.
DR VEuPathDB; FungiDB:An15g07890; -.
DR HOGENOM; CLU_1250404_0_0_1; -.
DR Proteomes; UP000006706; Chromosome 3R.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:1900818; P:ochratoxin A biosynthetic process; IDA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:GO_Central.
DR InterPro; IPR000837; AP-1.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR PANTHER; PTHR23351; PTHR23351; 1.
DR PRINTS; PR00042; LEUZIPPRFOS.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
PE 3: Inferred from homology;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..221
FT /note="Transcription factor otaR1"
FT /id="PRO_0000440593"
FT DOMAIN 152..215
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 109..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 152..192
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 198..212
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 111..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 221 AA; 24056 MW; 4DE28FD966FB4B64 CRC64;
MDGFEIASEL LQASHHLPVL GTGSPSGSDI ISGNYMLGYS ADDANLSQFE QLEGLLGSPT
VANHVDFGKE TADLAFLDDS GGILSSVELA GMVSPPLDAV VPMPGITRAS RSRPAFSTPA
SRPGLSSAKS PSLGATSPGS MDRSEEVKQL RKKYHEKYKE RNRLAAGRSR QKQADLINLL
QAEQQEEERR RKALELEIAN MQKELVDMKQ ELQHHIRISN C
