OTAY_ASPC5
ID OTAY_ASPC5 Reviewed; 123 AA.
AC A0A1R3RGJ9;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 03-AUG-2022, entry version 11.
DE RecName: Full=Probable cyclase otaY {ECO:0000303|PubMed:33391201};
DE EC=5.-.-.- {ECO:0000305|PubMed:33391201};
DE AltName: Full=Ochratoxin A biosynthesis cluster protein Y {ECO:0000303|PubMed:33391201};
GN ORFNames=ASPCADRAFT_209537;
OS Aspergillus carbonarius (strain ITEM 5010).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=602072;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ITEM 5010;
RX PubMed=28196534; DOI=10.1186/s13059-017-1151-0;
RA de Vries R.P., Riley R., Wiebenga A., Aguilar-Osorio G., Amillis S.,
RA Uchima C.A., Anderluh G., Asadollahi M., Askin M., Barry K., Battaglia E.,
RA Bayram O., Benocci T., Braus-Stromeyer S.A., Caldana C., Canovas D.,
RA Cerqueira G.C., Chen F., Chen W., Choi C., Clum A., Dos Santos R.A.,
RA Damasio A.R., Diallinas G., Emri T., Fekete E., Flipphi M., Freyberg S.,
RA Gallo A., Gournas C., Habgood R., Hainaut M., Harispe M.L., Henrissat B.,
RA Hilden K.S., Hope R., Hossain A., Karabika E., Karaffa L., Karanyi Z.,
RA Krasevec N., Kuo A., Kusch H., LaButti K., Lagendijk E.L., Lapidus A.,
RA Levasseur A., Lindquist E., Lipzen A., Logrieco A.F., MacCabe A.,
RA Maekelae M.R., Malavazi I., Melin P., Meyer V., Mielnichuk N., Miskei M.,
RA Molnar A.P., Mule G., Ngan C.Y., Orejas M., Orosz E., Ouedraogo J.P.,
RA Overkamp K.M., Park H.-S., Perrone G., Piumi F., Punt P.J., Ram A.F.,
RA Ramon A., Rauscher S., Record E., Riano-Pachon D.M., Robert V., Roehrig J.,
RA Ruller R., Salamov A., Salih N.S., Samson R.A., Sandor E., Sanguinetti M.,
RA Schuetze T., Sepcic K., Shelest E., Sherlock G., Sophianopoulou V.,
RA Squina F.M., Sun H., Susca A., Todd R.B., Tsang A., Unkles S.E.,
RA van de Wiele N., van Rossen-Uffink D., Oliveira J.V., Vesth T.C.,
RA Visser J., Yu J.-H., Zhou M., Andersen M.R., Archer D.B., Baker S.E.,
RA Benoit I., Brakhage A.A., Braus G.H., Fischer R., Frisvad J.C.,
RA Goldman G.H., Houbraken J., Oakley B., Pocsi I., Scazzocchio C.,
RA Seiboth B., vanKuyk P.A., Wortman J., Dyer P.S., Grigoriev I.V.;
RT "Comparative genomics reveals high biological diversity and specific
RT adaptations in the industrially and medically important fungal genus
RT Aspergillus.";
RL Genome Biol. 18:RESEARCH28.1-RESEARCH28.45(2017).
RN [2]
RP IDENTIFICATION, FUNCTION, AND PATHWAY.
RX PubMed=33391201; DOI=10.3389/fmicb.2020.581309;
RA Ferrara M., Gallo A., Perrone G., Magista D., Baker S.E.;
RT "Comparative genomic analysis of ochratoxin A biosynthetic cluster in
RT producing fungi: new evidence of a cyclase gene involvement.";
RL Front. Microbiol. 11:581309-581309(2020).
CC -!- FUNCTION: Probable cyclase; part of the gene cluster that mediates the
CC biosynthesis of ochratoxin A (OTA), a mycotoxin composed of a
CC chlorinated type I polyketide dihydroisocoumarin moiety linked to L-
CC phenylalanine, and demonstrated to have nephrotoxic, immunotoxic,
CC genotoxic, neurotoxic, and teratogenic properties (PubMed:33391201).
CC OtaY is probably involved in the polyketide cyclization
CC (PubMed:33391201) (Probable). The pathway begins with the highly
CC reducing polyketide synthase otaA that catalyzes the formation of the
CC isocoumarin group during the initial stages of biosynthesis, starting
CC from one acetate and 4 malonate units, to originate the characteristic
CC pentaketide skeleton 7-methylmellein (7-MM) of the OTA molecule. The
CC newly identified cyclase otaY might be involved in the polyketide
CC cyclization reaction during the initial steps of the OTA biosynthesis.
CC 7-MM is then oxidized into 7-carboxymellein (also called ochratoxin
CC beta) by the cytochrome P450 monooxygenase otaC. The NRPS encoded by
CC the otaB gene is involved in the linking of phenylalanine to the
CC dihydroisocoumarin ring. The reaction catalyzed by NRPS results in the
CC production of ochratoxin B (OTB), which is the non-chlorinated analog
CC of OTA and which subsequently serves as the substrate of the halogenase
CC otaD for chlorination activity to form the final molecular structure of
CC OTA, containing a chlorine atom in the C-5 position of the molecule
CC (PubMed:33391201) (Probable). {ECO:0000269|PubMed:33391201,
CC ECO:0000305|PubMed:33391201}.
CC -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:33391201}.
CC -!- SIMILARITY: Belongs to the aurE cyclase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=OOF93600.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; KV907504; OOF93600.1; ALT_TERM; Genomic_DNA.
DR SMR; A0A1R3RGJ9; -.
DR EnsemblFungi; OOF93600; OOF93600; ASPCADRAFT_209537.
DR VEuPathDB; FungiDB:ASPCADRAFT_209537; -.
DR OrthoDB; 1630654at2759; -.
DR Proteomes; UP000188318; Unassembled WGS sequence.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:1900818; P:ochratoxin A biosynthetic process; IEP:GO_Central.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR037401; SnoaL-like.
DR Pfam; PF12680; SnoaL_2; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome.
FT CHAIN 1..123
FT /note="Probable cyclase otaY"
FT /id="PRO_0000453469"
SQ SEQUENCE 123 AA; 13873 MW; E9D5F035E2599191 CRC64;
MTTPTPTTLK HHATTILTTL VNDRQTAAIE HLLHPTITLK HNDLPAMSKS ELIAFWPEVL
AQSPHFRVQI RDVIAEGNKV WVYSRVEGRL GEGVMDDFHM MVFDEEGLVV RSTGVQRVVE
GEM
