ID   OTAY_ASPNC              Reviewed;         119 AA.
AC   P0DUR9;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   03-AUG-2022, entry version 4.
DE   RecName: Full=Probable cyclase otaY {ECO:0000303|PubMed:33391201};
DE            EC=5.-.-.- {ECO:0000305|PubMed:33391201};
DE   AltName: Full=Ochratoxin A biosynthesis cluster protein Y {ECO:0000303|PubMed:33391201};
OS   Aspergillus niger (strain CBS 513.88 / FGSC A1513).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=425011;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 513.88 / FGSC A1513 / ATCC MYA-4892;
RX   PubMed=17259976; DOI=10.1038/nbt1282;
RA   Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J.,
RA   Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R.,
RA   Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X.,
RA   Contreras R., Cornell M., Coutinho P.M., Danchin E.G.J., Debets A.J.M.,
RA   Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M.,
RA   d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J.,
RA   Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W.,
RA   van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M.,
RA   Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X.,
RA   van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A.,
RA   Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E.,
RA   Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J.,
RA   Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H.,
RA   Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.;
RT   "Genome sequencing and analysis of the versatile cell factory Aspergillus
RT   niger CBS 513.88.";
RL   Nat. Biotechnol. 25:221-231(2007).
RN   [2]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=27667988; DOI=10.3389/fmicb.2016.01412;
RA   Susca A., Proctor R.H., Morelli M., Haidukowski M., Gallo A.,
RA   Logrieco A.F., Moretti A.;
RT   "Variation in fumonisin and ochratoxin production associated with
RT   differences in biosynthetic gene content in Aspergillus niger and A.
RT   welwitschiae isolates from multiple crop and geographic origins.";
RL   Front. Microbiol. 7:1412-1412(2016).
RN   [3]
RP   IDENTIFICATION, FUNCTION, AND PATHWAY.
RX   PubMed=33391201; DOI=10.3389/fmicb.2020.581309;
RA   Ferrara M., Gallo A., Perrone G., Magista D., Baker S.E.;
RT   "Comparative genomic analysis of ochratoxin A biosynthetic cluster in
RT   producing fungi: new evidence of a cyclase gene involvement.";
RL   Front. Microbiol. 11:581309-581309(2020).
CC   -!- FUNCTION: Probable cyclase; part of the gene cluster that mediates the
CC       biosynthesis of ochratoxin A (OTA), a mycotoxin composed of a
CC       chlorinated type I polyketide dihydroisocoumarin moiety linked to L-
CC       phenylalanine, and demonstrated to have nephrotoxic, immunotoxic,
CC       genotoxic, neurotoxic, and teratogenic properties (PubMed:33391201).
CC       OtaY is probably involved in the polyketide cyclization
CC       (PubMed:33391201) (Probable). The pathway begins with the highly
CC       reducing polyketide synthase otaA that catalyzes the formation of the
CC       isocoumarin group during the initial stages of biosynthesis, starting
CC       from one acetate and 4 malonate units, to originate the characteristic
CC       pentaketide skeleton 7-methylmellein (7-MM) of the OTA molecule. The
CC       newly identified cyclase otaY might be involved in the polyketide
CC       cyclization reaction during the initial steps of the OTA biosynthesis.
CC       7-MM is then oxidized into 7-carboxymellein (also called ochratoxin
CC       beta) by the cytochrome P450 monooxygenase otaC. The NRPS encoded by
CC       the otaB gene is involved in the linking of phenylalanine to the
CC       dihydroisocoumarin ring. The reaction catalyzed by NRPS results in the
CC       production of ochratoxin B (OTB), which is the non-chlorinated analog
CC       of OTA and which subsequently serves as the substrate of the halogenase
CC       otaD for chlorination activity to form the final molecular structure of
CC       OTA, containing a chlorine atom in the C-5 position of the molecule
CC       (PubMed:27667988, PubMed:33391201) (Probable).
CC       {ECO:0000269|PubMed:33391201, ECO:0000305|PubMed:27667988,
CC       ECO:0000305|PubMed:33391201}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:33391201}.
CC   -!- SIMILARITY: Belongs to the aurE cyclase family. {ECO:0000305}.
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DR   EMBL; AM270352; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0DUR9; -.
DR   Proteomes; UP000006706; Chromosome 3R.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:1900818; P:ochratoxin A biosynthetic process; ISS:GO_Central.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR037401; SnoaL-like.
DR   Pfam; PF12680; SnoaL_2; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome.
FT   CHAIN           1..119
FT                   /note="Probable cyclase otaY"
FT                   /id="PRO_0000453470"
SQ   SEQUENCE   119 AA;  13700 MW;  62BF84223D03D3DF CRC64;
     MDLKSRAETF LTTIVNRRET ASIEHYLHPD AQFKHNDLPS MSTAELLAFW PQVLDESPDF
     RVQILATIAE GLRVWVYSRV EGRLGKGVMD DVHMLEFDGN GLLIRSRGIQ REVVEEMEK