OTBP_DANRE
ID OTBP_DANRE Reviewed; 315 AA.
AC Q6DGH9; Q9W7M5;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Homeobox protein orthopedia B;
GN Name=otpb; Synonyms=otp, otp1, otpa;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=17074092; DOI=10.1186/1471-213x-6-50;
RA Del Giacco L., Sordino P., Pistocchi A., Andreakis N., Tarallo R.,
RA Di Benedetto B., Cotelli F.;
RT "Differential regulation of the zebrafish orthopedia 1 gene during fate
RT determination of diencephalic neurons.";
RL BMC Dev. Biol. 6:50-50(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=17481897; DOI=10.1016/j.cub.2007.04.003;
RA Ryu S., Mahler J., Acampora D., Holzschuh J., Erhardt S., Omodei D.,
RA Simeone A., Driever W.;
RT "Orthopedia homeodomain protein is essential for diencephalic dopaminergic
RT neuron development.";
RL Curr. Biol. 17:873-880(2007).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=17180684; DOI=10.1007/s00427-006-0123-2;
RA Eaton J.L., Glasgow E.;
RT "Zebrafish orthopedia (otp) is required for isotocin cell development.";
RL Dev. Genes Evol. 217:149-158(2007).
CC -!- FUNCTION: Involved in the specification of isotocin cells. Required for
CC the specification of dopaminergic neurons in the developing
CC hypothalamus and posterior tuberculum. {ECO:0000269|PubMed:17180684,
CC ECO:0000269|PubMed:17481897}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00138, ECO:0000269|PubMed:17180684}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6DGH9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6DGH9-2; Sequence=VSP_026418;
CC -!- TISSUE SPECIFICITY: Isoform 1 and isoform 2 are expressed in brain and
CC testis. {ECO:0000269|PubMed:17074092}.
CC -!- DEVELOPMENTAL STAGE: Isoform 1 and isoform 2 are expressed during
CC embryonic development. At 16 hpf, expressed in a few cells in the
CC ventral dieencephalon. At 48 hpf, present in the preoptic area and
CC ventral hypothalamus (at protein level). {ECO:0000269|PubMed:17074092,
CC ECO:0000269|PubMed:17180684}.
CC -!- MISCELLANEOUS: [Isoform 2]: Characteristic of the fish lineage.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; AF071496; AAD42021.1; -; mRNA.
DR EMBL; BC076366; AAH76366.1; -; mRNA.
DR RefSeq; NP_571175.1; NM_131100.1. [Q6DGH9-2]
DR RefSeq; XP_005165562.1; XM_005165505.2. [Q6DGH9-1]
DR AlphaFoldDB; Q6DGH9; -.
DR SMR; Q6DGH9; -.
DR STRING; 7955.ENSDARP00000122985; -.
DR PaxDb; Q6DGH9; -.
DR Ensembl; ENSDART00000081249; ENSDARP00000075692; ENSDARG00000058379. [Q6DGH9-1]
DR Ensembl; ENSDART00000134606; ENSDARP00000122985; ENSDARG00000058379. [Q6DGH9-2]
DR GeneID; 30316; -.
DR KEGG; dre:30316; -.
DR CTD; 30316; -.
DR ZFIN; ZDB-GENE-990708-7; otpb.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000159952; -.
DR HOGENOM; CLU_056068_0_0_1; -.
DR InParanoid; Q6DGH9; -.
DR OMA; ERCFNRT; -.
DR OrthoDB; 1243020at2759; -.
DR PhylomeDB; Q6DGH9; -.
DR TreeFam; TF351614; -.
DR PRO; PR:Q6DGH9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 5.
DR Bgee; ENSDARG00000058379; Expressed in forebrain and 55 other tissues.
DR ExpressionAtlas; Q6DGH9; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0021884; P:forebrain neuron development; IGI:ZFIN.
DR GO; GO:0021854; P:hypothalamus development; IMP:ZFIN.
DR GO; GO:0021767; P:mammillary body development; IGI:ZFIN.
DR GO; GO:0030182; P:neuron differentiation; IMP:ZFIN.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; DNA-binding;
KW Homeobox; Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..315
FT /note="Homeobox protein orthopedia B"
FT /id="PRO_0000292433"
FT DNA_BIND 99..158
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 33..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 296..309
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT COMPBIAS 59..97
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 13
FT /note="G -> GELSPPNSGLQLHW (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17074092"
FT /id="VSP_026418"
SQ SEQUENCE 315 AA; 34028 MW; A25FFD874CC4AA00 CRC64;
MLSHADLLDT RLGMKDAAAE LLVHREALKC RLGGTDSGHP GDLTSATETV EGTTLLPGEE
ISNGGSNPNG MQQVNAKDQE KQQQQNSNQT GGQQNQQKQK RHRTRFTPAQ LNELERSFAK
THYPDIFMRE ELALRIGLTE SRVQVWFQNR RAKWKKRKKT TNVFRAPGTL LPTHGLPQFP
SAAAAAMGDS LCSFHANDTR WAAAGMPGVS QLQIPPSLGR QQAMAQSLSQ CSLGAGPPPN
NMGLSGSLSS NGSGLQSHLY QPTFPGMVPA SLSGPTNVTG SPQLCSSPDS DMWRGTSIAS
LRRKALEHTV SMSFT
