OTC_NEIPE
ID OTC_NEIPE Reviewed; 232 AA.
AC O86404; O86405;
DT 11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Ornithine carbamoyltransferase;
DE Short=OTCase;
DE EC=2.1.3.3;
DE Flags: Fragment;
GN Name=argF;
OS Neisseria perflava.
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=33053;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10555 / DSM 18009 / CIP 73.11 / CCUG 17915 / LMG 5284 / NRL
RC 30015 / NRRL B-1790, and LCDC 85402;
RX PubMed=10368955; DOI=10.1093/oxfordjournals.molbev.a026162;
RA Smith N.H., Holmes E.C., Donovan G.M., Carpenter G.A., Spratt B.G.;
RT "Networks and groups within the genus Neisseria: analysis of argF, recA,
RT rho, and 16S rRNA sequences from human Neisseria species.";
RL Mol. Biol. Evol. 16:773-783(1999).
CC -!- FUNCTION: Reversibly catalyzes the transfer of the carbamoyl group from
CC carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to
CC produce L-citrulline. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=carbamoyl phosphate + L-ornithine = H(+) + L-citrulline +
CC phosphate; Xref=Rhea:RHEA:19513, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:46911, ChEBI:CHEBI:57743,
CC ChEBI:CHEBI:58228; EC=2.1.3.3;
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC from L-ornithine and carbamoyl phosphate: step 1/3.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the aspartate/ornithine carbamoyltransferase
CC superfamily. OTCase family. {ECO:0000305}.
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DR EMBL; AJ223883; CAA11614.1; -; Genomic_DNA.
DR EMBL; AJ223897; CAA11628.1; -; Genomic_DNA.
DR AlphaFoldDB; O86404; -.
DR SMR; O86404; -.
DR UniPathway; UPA00068; UER00112.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR GO; GO:0004585; F:ornithine carbamoyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1370; -; 2.
DR InterPro; IPR006132; Asp/Orn_carbamoyltranf_P-bd.
DR InterPro; IPR006130; Asp/Orn_carbamoylTrfase.
DR InterPro; IPR036901; Asp/Orn_carbamoylTrfase_sf.
DR InterPro; IPR006131; Asp_carbamoyltransf_Asp/Orn-bd.
DR InterPro; IPR002292; Orn/put_carbamltrans.
DR Pfam; PF00185; OTCace; 1.
DR Pfam; PF02729; OTCace_N; 1.
DR PRINTS; PR00100; AOTCASE.
DR PRINTS; PR00102; OTCASE.
DR SUPFAM; SSF53671; SSF53671; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Arginine biosynthesis; Cytoplasm; Transferase.
FT CHAIN <1..>232
FT /note="Ornithine carbamoyltransferase"
FT /id="PRO_0000112968"
FT BINDING 15
FT /ligand="carbamoyl phosphate"
FT /ligand_id="ChEBI:CHEBI:58228"
FT /evidence="ECO:0000250"
FT BINDING 39
FT /ligand="carbamoyl phosphate"
FT /ligand_id="ChEBI:CHEBI:58228"
FT /evidence="ECO:0000250"
FT BINDING 66..69
FT /ligand="carbamoyl phosphate"
FT /ligand_id="ChEBI:CHEBI:58228"
FT /evidence="ECO:0000250"
FT BINDING 99
FT /ligand="L-ornithine"
FT /ligand_id="ChEBI:CHEBI:46911"
FT /evidence="ECO:0000250"
FT BINDING 163
FT /ligand="L-ornithine"
FT /ligand_id="ChEBI:CHEBI:46911"
FT /evidence="ECO:0000250"
FT BINDING 167..168
FT /ligand="L-ornithine"
FT /ligand_id="ChEBI:CHEBI:46911"
FT /evidence="ECO:0000250"
FT BINDING 204..207
FT /ligand="carbamoyl phosphate"
FT /ligand_id="ChEBI:CHEBI:58228"
FT /evidence="ECO:0000250"
FT BINDING 232
FT /ligand="carbamoyl phosphate"
FT /ligand_id="ChEBI:CHEBI:58228"
FT /evidence="ECO:0000250"
FT SITE 79
FT /note="Important for structural integrity"
FT /evidence="ECO:0000250"
FT VARIANT 6
FT /note="A -> V (in strain: CCUG 17915L)"
FT VARIANT 33..35
FT /note="YDA -> FDG (in strain: CCUG 17915L)"
FT VARIANT 44..46
FT /note="EII -> DVV (in strain: CCUG 17915L)"
FT VARIANT 82
FT /note="S -> N (in strain: CCUG 17915L)"
FT VARIANT 88
FT /note="T -> I (in strain: CCUG 17915L)"
FT VARIANT 101
FT /note="G -> A (in strain: CCUG 17915L)"
FT VARIANT 106..108
FT /note="ILG -> VLA (in strain: CCUG 17915L)"
FT VARIANT 121
FT /note="E -> K (in strain: CCUG 17915L)"
FT VARIANT 128..134
FT /note="GIIAAAH -> NIIETVQ (in strain: CCUG 17915L)"
FT VARIANT 142..145
FT /note="AKIT -> GRIL (in strain: CCUG 17915L)"
FT VARIANT 150..151
FT /note="AH -> VK (in strain: CCUG 17915L)"
FT VARIANT 155
FT /note="N -> K (in strain: CCUG 17915L)"
FT VARIANT 158
FT /note="G -> D (in strain: CCUG 17915L)"
FT VARIANT 174
FT /note="V -> A (in strain: CCUG 17915L)"
FT VARIANT 195..196
FT /note="SG -> AE (in strain: CCUG 17915L)"
FT NON_TER 1
FT NON_TER 232
SQ SEQUENCE 232 AA; 25480 MW; E750AF7DD8FA3816 CRC64;
DQGAGATYLE PSASQIGHKE SIKDTARVLG RMYDAIEYRG FGQEIIEELA KYAGVPVFNG
LTNEFHPTQM LADALTMREH SSKPLNQTAF AYVGDARYNM GNSLLILGAK LGMDVRIGAP
ESLWPSEGII AAAHAVAKET GAKITLTENA HEAVNGVGFI HTDVWVSMGE PKEVWQERID
LLKDYRVTPE LMAASGNPQV KFMHCLPAFH NRETKVGEWI YETFGLNGVE VT
