ASCG_ECOLI
ID ASCG_ECOLI Reviewed; 336 AA.
AC P24242; Q2MAB5; Q46879;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 4.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=HTH-type transcriptional regulator AscG;
DE AltName: Full=Cryptic asc operon repressor;
GN Name=ascG; OrderedLocusNames=b2714, JW5434;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1630307; DOI=10.1093/oxfordjournals.molbev.a040753;
RA Hall B.G., Xu L.;
RT "Nucleotide sequence, function, activation, and evolution of the cryptic
RT asc operon of Escherichia coli K12.";
RL Mol. Biol. Evol. 9:688-706(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Repressor of the asc operon. The cryptic operon is activated
CC by the insertion of IS186 into the ascG gene.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA16428.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAA69224.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE76791.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M73326; AAA16428.1; ALT_INIT; Unassigned_DNA.
DR EMBL; U29579; AAA69224.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC75756.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76791.1; ALT_INIT; Genomic_DNA.
DR PIR; F65051; F65051.
DR RefSeq; NP_417194.2; NC_000913.3.
DR RefSeq; WP_001394680.1; NZ_LN832404.1.
DR PDB; 3BRQ; X-ray; 2.00 A; A/B=43-336.
DR PDB; 3DBI; X-ray; 2.45 A; A/B/C=2-336.
DR PDBsum; 3BRQ; -.
DR PDBsum; 3DBI; -.
DR AlphaFoldDB; P24242; -.
DR SMR; P24242; -.
DR BioGRID; 4259426; 8.
DR BioGRID; 851633; 15.
DR IntAct; P24242; 14.
DR STRING; 511145.b2714; -.
DR PaxDb; P24242; -.
DR PRIDE; P24242; -.
DR EnsemblBacteria; AAC75756; AAC75756; b2714.
DR EnsemblBacteria; BAE76791; BAE76791; BAE76791.
DR GeneID; 947305; -.
DR KEGG; ecj:JW5434; -.
DR KEGG; eco:b2714; -.
DR PATRIC; fig|511145.12.peg.2806; -.
DR EchoBASE; EB0085; -.
DR eggNOG; COG1609; Bacteria.
DR HOGENOM; CLU_037628_6_0_6; -.
DR InParanoid; P24242; -.
DR OMA; SHCVWSD; -.
DR PhylomeDB; P24242; -.
DR BioCyc; EcoCyc:EG10087-MON; -.
DR EvolutionaryTrace; P24242; -.
DR PRO; PR:P24242; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:EcoCyc.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEP:EcoCyc.
DR CDD; cd01392; HTH_LacI; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR000843; HTH_LacI.
DR InterPro; IPR046335; LacI/GalR-like_sensor.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR028082; Peripla_BP_I.
DR Pfam; PF00356; LacI; 1.
DR Pfam; PF13377; Peripla_BP_3; 1.
DR SMART; SM00354; HTH_LACI; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00356; HTH_LACI_1; 1.
DR PROSITE; PS50932; HTH_LACI_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..336
FT /note="HTH-type transcriptional regulator AscG"
FT /id="PRO_0000107923"
FT DOMAIN 2..56
FT /note="HTH lacI-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT DNA_BIND 4..23
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT CONFLICT 207..230
FT /note="QHGIALNEKLIANGKWTPASGAEG -> SMVLRSMKNLSLTVNGRLPAGRR
FT (in Ref. 1; AAA16428)"
FT /evidence="ECO:0000305"
FT STRAND 61..66
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 76..89
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 93..97
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 103..115
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 119..124
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 126..128
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 130..138
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 144..148
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 152..154
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 155..157
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 163..176
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 181..185
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 192..206
FT /evidence="ECO:0007829|PDB:3BRQ"
FT TURN 207..209
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 214..216
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 224..235
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 242..248
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 249..262
FT /evidence="ECO:0007829|PDB:3BRQ"
FT TURN 266..269
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 271..276
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 281..283
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 284..286
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 289..292
FT /evidence="ECO:0007829|PDB:3BRQ"
FT HELIX 295..310
FT /evidence="ECO:0007829|PDB:3BRQ"
FT STRAND 324..326
FT /evidence="ECO:0007829|PDB:3BRQ"
SQ SEQUENCE 336 AA; 36813 MW; A2825E9E4DF6A261 CRC64;
MTTMLEVAKR AGVSKATVSR VLSGNGYVSQ ETKDRVFQAV EESGYRPNLL ARNLSAKSTQ
TLGLVVTNTL YHGIYFSELL FHAARMAEEK GRQLLLADGK HSAEEERQAI QYLLDLRCDA
IMIYPRFLSV DEIDDIIDAH SQPIMVLNRR LRKNSSHSVW CDHKQTSFNA VAELINAGHQ
EIAFLTGSMD SPTSIERLAG YKDALAQHGI ALNEKLIANG KWTPASGAEG VEMLLERGAK
FSALVASNDD MAIGAMKALH ERGVAVPEQV SVIGFDDIAI APYTVPALSS VKIPVTEMIQ
EIIGRLIFML DGGDFSPPKT FSGKLIRRDS LIAPSR
