ASCL1_RAT
ID ASCL1_RAT Reviewed; 233 AA.
AC P19359;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Achaete-scute homolog 1;
GN Name=Ascl1; Synonyms=Ash1, Mash-1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Sprague-Dawley;
RX PubMed=2392153; DOI=10.1038/346858a0;
RA Johnson J.E., Birren S.J., Anderson D.J.;
RT "Two rat homologues of Drosophila achaete-scute specifically expressed in
RT neuronal precursors.";
RL Nature 346:858-861(1990).
RN [2]
RP FUNCTION.
RX PubMed=10648228; DOI=10.1242/dev.127.4.693;
RA Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.;
RT "Generation of neurons by transient expression of neural bHLH proteins in
RT mammalian cells.";
RL Development 127:693-702(2000).
CC -!- FUNCTION: Transcription factor that plays a key role in neuronal
CC differentiation: acts as a pioneer transcription factor, accessing
CC closed chromatin to allow other factors to bind and activate neural
CC pathways (PubMed:10648228). Directly binds the E box motif (5'-CANNTG-
CC 3') on promoters and promotes transcription of neuronal genes. The
CC combination of three transcription factors, ASCL1, POU3F2/BRN2 and
CC MYT1L, is sufficient to reprogram fibroblasts and other somatic cells
CC into induced neuronal (iN) cells in vitro. Plays a role at early stages
CC of development of specific neural lineages in most regions of the CNS,
CC and of several lineages in the PNS. Essential for the generation of
CC olfactory and autonomic neurons. Acts synergistically with FOXN4 to
CC specify the identity of V2b neurons rather than V2a from bipotential p2
CC progenitors during spinal cord neurogenesis, probably through DLL4-
CC NOTCH signaling activation. Involved in the regulation of
CC neuroendocrine cell development in the glandular stomach (By
CC similarity). {ECO:0000250|UniProtKB:Q02067,
CC ECO:0000269|PubMed:10648228}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Forms a heterodimer with TCF3. {ECO:0000250|UniProtKB:P50553}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q02067}.
CC -!- TISSUE SPECIFICITY: Developing CNS and PNS at embryonic and postnatal
CC stages. {ECO:0000269|PubMed:2392153}.
CC -!- DEVELOPMENTAL STAGE: It is first observed after neurulation, in 10.5
CC day old (E10.5) rat embryos, and is restricted to subsets of
CC neuroepithelial cells in the spinal cord and the brain, between E10.5
CC and E13.5. In the periphery, its expression is restricted to some
CC lineages of neural crest-derived cells, namely in sympathetic and
CC enteric neural precursors. In the PNS its expression is extinguished at
CC or before differentiation. {ECO:0000269|PubMed:2392153}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X53725; CAA37760.1; -; mRNA.
DR PIR; S11563; S11563.
DR RefSeq; NP_071779.1; NM_022384.1.
DR AlphaFoldDB; P19359; -.
DR SMR; P19359; -.
DR IntAct; P19359; 1.
DR MINT; P19359; -.
DR STRING; 10116.ENSRNOP00000005674; -.
DR PhosphoSitePlus; P19359; -.
DR PaxDb; P19359; -.
DR Ensembl; ENSRNOT00000005674; ENSRNOP00000005674; ENSRNOG00000004294.
DR GeneID; 64186; -.
DR KEGG; rno:64186; -.
DR UCSC; RGD:71010; rat.
DR CTD; 429; -.
DR RGD; 71010; Ascl1.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000162483; -.
DR HOGENOM; CLU_063523_3_0_1; -.
DR InParanoid; P19359; -.
DR OMA; QLIPPAC; -.
DR OrthoDB; 1131543at2759; -.
DR PhylomeDB; P19359; -.
DR TreeFam; TF322889; -.
DR PRO; PR:P19359; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000004294; Expressed in thymus and 6 other tissues.
DR Genevisible; P19359; RN.
DR GO; GO:0043025; C:neuronal cell body; ISO:RGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0043425; F:bHLH transcription factor binding; ISO:RGD.
DR GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:RGD.
DR GO; GO:0070888; F:E-box binding; ISO:RGD.
DR GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0061104; P:adrenal chromaffin cell differentiation; ISO:RGD.
DR GO; GO:0061103; P:carotid body glomus cell differentiation; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:0048469; P:cell maturation; ISO:RGD.
DR GO; GO:0071259; P:cellular response to magnetism; IEP:RGD.
DR GO; GO:0021954; P:central nervous system neuron development; ISO:RGD.
DR GO; GO:0021987; P:cerebral cortex development; ISO:RGD.
DR GO; GO:0021892; P:cerebral cortex GABAergic interneuron differentiation; ISO:RGD.
DR GO; GO:0021902; P:commitment of neuronal cell to specific neuron type in forebrain; ISO:RGD.
DR GO; GO:0021879; P:forebrain neuron differentiation; IDA:RGD.
DR GO; GO:0048699; P:generation of neurons; ISO:RGD.
DR GO; GO:0010001; P:glial cell differentiation; ISO:RGD.
DR GO; GO:0007507; P:heart development; IEP:RGD.
DR GO; GO:0061100; P:lung neuroendocrine cell differentiation; ISO:RGD.
DR GO; GO:0097475; P:motor neuron migration; IEA:Ensembl.
DR GO; GO:0050883; P:musculoskeletal movement, spinal reflex action; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IDA:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0007399; P:nervous system development; IDA:RGD.
DR GO; GO:0007400; P:neuroblast fate determination; ISO:RGD.
DR GO; GO:0007405; P:neuroblast proliferation; ISO:RGD.
DR GO; GO:0022008; P:neurogenesis; ISO:RGD.
DR GO; GO:0048666; P:neuron development; ISS:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; ISO:RGD.
DR GO; GO:0048663; P:neuron fate commitment; ISS:UniProtKB.
DR GO; GO:0048665; P:neuron fate specification; ISS:UniProtKB.
DR GO; GO:0001764; P:neuron migration; ISO:RGD.
DR GO; GO:0003358; P:noradrenergic neuron development; ISS:UniProtKB.
DR GO; GO:0003359; P:noradrenergic neuron fate commitment; ISO:RGD.
DR GO; GO:0007219; P:Notch signaling pathway; ISO:RGD.
DR GO; GO:0060166; P:olfactory pit development; ISO:RGD.
DR GO; GO:0021779; P:oligodendrocyte cell fate commitment; ISO:RGD.
DR GO; GO:0014003; P:oligodendrocyte development; ISO:RGD.
DR GO; GO:0048709; P:oligodendrocyte differentiation; ISO:RGD.
DR GO; GO:0007389; P:pattern specification process; ISO:RGD.
DR GO; GO:0048935; P:peripheral nervous system neuron development; IEA:Ensembl.
DR GO; GO:0045787; P:positive regulation of cell cycle; ISO:RGD.
DR GO; GO:2000179; P:positive regulation of neural precursor cell proliferation; ISO:RGD.
DR GO; GO:0050769; P:positive regulation of neurogenesis; ISO:RGD.
DR GO; GO:0043525; P:positive regulation of neuron apoptotic process; ISO:RGD.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:UniProtKB.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0030856; P:regulation of epithelial cell differentiation; ISO:RGD.
DR GO; GO:0010468; P:regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; ISO:RGD.
DR GO; GO:0050767; P:regulation of neurogenesis; ISO:RGD.
DR GO; GO:0008593; P:regulation of Notch signaling pathway; ISO:RGD.
DR GO; GO:0060165; P:regulation of timing of subpallium neuron differentiation; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0070849; P:response to epidermal growth factor; IEP:RGD.
DR GO; GO:0051593; P:response to folic acid; IEP:RGD.
DR GO; GO:0010226; P:response to lithium ion; IEP:RGD.
DR GO; GO:0032526; P:response to retinoic acid; IEP:RGD.
DR GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR GO; GO:0021527; P:spinal cord association neuron differentiation; ISO:RGD.
DR GO; GO:0021529; P:spinal cord oligodendrocyte cell differentiation; ISO:RGD.
DR GO; GO:0021530; P:spinal cord oligodendrocyte cell fate specification; ISO:RGD.
DR GO; GO:0061102; P:stomach neuroendocrine cell differentiation; ISO:RGD.
DR GO; GO:0060163; P:subpallium neuron fate commitment; ISO:RGD.
DR GO; GO:0061549; P:sympathetic ganglion development; ISS:UniProtKB.
DR GO; GO:0060579; P:ventral spinal cord interneuron fate commitment; ISS:UniProtKB.
DR GO; GO:0021750; P:vestibular nucleus development; ISO:RGD.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Activator; Developmental protein; Differentiation;
KW DNA-binding; Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..233
FT /note="Achaete-scute homolog 1"
FT /id="PRO_0000127128"
FT DOMAIN 115..167
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 39..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 153
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q02067"
SQ SEQUENCE 233 AA; 24972 MW; 036BDAC8E2D23274 CRC64;
MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAAQSAQQQ QQQQAPQQQA
PQLSPVADGQ PSGGGHKSAA KQVKRQRSSS PELMRCKRRL NFSGFGYSLP QQQPAAVARR
NERERNRVKL VNLGFATLRE HVPNGAANKK MSKVETLRSA VEYIRALQQL LDEHDAVSAA
FQAGVLSPTI SPNYSNDLNS MAGSPVSSYS SDEGSYDPLS PEEQELLDFT NWF
