ID   ASCL2_ORYSJ             Reviewed;         303 AA.
AC   Q6YWS8;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 2.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=ASC1-like protein 2;
DE   AltName: Full=Alternaria stem canker resistance-like protein 2;
GN   OrderedLocusNames=Os02g0728300, LOC_Os02g49590;
GN   ORFNames=B1121A12.39, OSJNBa0072H09.2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Mediates resistance to sphinganine-analog mycotoxins (SAMs)
CC       by restoring the sphingolipid biosynthesis. Could salvage the transport
CC       of GPI-anchored proteins from the endoplasmic reticulum to the Golgi
CC       apparatus in ceramides-depleted cells after SAM exposure (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD16097.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAD16340.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP005284; BAD16097.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP005751; BAD16340.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; Q6YWS8; -.
DR   SMR; Q6YWS8; -.
DR   STRING; 4530.OS02T0728300-00; -.
DR   iPTMnet; Q6YWS8; -.
DR   PaxDb; Q6YWS8; -.
DR   PRIDE; Q6YWS8; -.
DR   eggNOG; KOG1607; Eukaryota.
DR   HOGENOM; CLU_028277_5_0_1; -.
DR   InParanoid; Q6YWS8; -.
DR   PlantReactome; R-OSA-1119325; Sphingolipid metabolism.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q6YWS8; OS.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016410; F:N-acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0050291; F:sphingosine N-acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0046513; P:ceramide biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR016439; Lag1/Lac1-like.
DR   InterPro; IPR006634; TLC-dom.
DR   PANTHER; PTHR12560; PTHR12560; 1.
DR   Pfam; PF03798; TRAM_LAG1_CLN8; 1.
DR   SMART; SM00724; TLC; 1.
DR   PROSITE; PS50922; TLC; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..303
FT                   /note="ASC1-like protein 2"
FT                   /id="PRO_0000185524"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        208..228
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        253..273
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          68..282
FT                   /note="TLC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00205"
SQ   SEQUENCE   303 AA;  35584 MW;  3D7B5F7E5421E9A1 CRC64;
     MGVPPVDWEA ESYPAYSDFA AIPLFAVFLF AVRYLLDRFV FEWLARRLIF EKDEKLDLAT
     HAGRIKIRKF KESAWKCIYF LSAELLALSV TYKESWFTST KNFWVGPGDQ VWPDQRIKFK
     LKLVYMYAAG FYTYSIFALQ FWEIKRSDFG ISMVHHVVSV ILIALSYIFR FARVGSIVLA
     IHDASDVFLE LGKISKYSGY QLLADVSFLI FVCSWAVLRL IYYPFWILWS TSYEVVPMLD
     KKKHKFDGPL HYYVFNCLLF SLLVLNIYWW VLMYRMLVEQ ILSKGHVGDD VRSGRFSPPF
     IPP