OTOP2_MOUSE
ID OTOP2_MOUSE Reviewed; 563 AA.
AC Q80SX5; Q8CE77;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Proton channel OTOP2 {ECO:0000305};
DE AltName: Full=Otopetrin-2 {ECO:0000303|PubMed:12651873};
GN Name=Otop2 {ECO:0000303|PubMed:12651873, ECO:0000312|MGI:MGI:2388365};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Kikkawa Y., Shitara H., Kohara Y., Takada T., Okamoto M., Taya C.,
RA Wakana S., Kamiya K., Yoshikawa Y., Tokano H., Kitamura K., Shimizu K.,
RA Shiroishi T., Wakabayashi Y., Kominami R., Yonekawa H.;
RT "Mutations in a new protein containing ankyrin repeats and SAM domain cause
RT deafness in Jackson shaker mice, a model for Usher syndrome type IG.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP IDENTIFICATION.
RC STRAIN=C57BL/6J;
RX PubMed=12651873; DOI=10.1093/hmg/ddg087;
RA Hurle B., Ignatova E., Massironi S.M., Mashimo T., Rios X., Thalmann I.,
RA Thalmann R., Ornitz D.M.;
RT "Non-syndromic vestibular disorder with otoconial agenesis in
RT tilted/mergulhador mice caused by mutations in otopetrin 1.";
RL Hum. Mol. Genet. 12:777-789(2003).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=29371428; DOI=10.1126/science.aao3264;
RA Tu Y.H., Cooper A.J., Teng B., Chang R.B., Artiga D.J., Turner H.N.,
RA Mulhall E.M., Ye W., Smith A.D., Liman E.R.;
RT "An evolutionarily conserved gene family encodes proton-selective ion
RT channels.";
RL Science 359:1047-1050(2018).
CC -!- FUNCTION: Proton-selective channel that specifically transports protons
CC into cells. Proton-selective channel activity is probably required in
CC cell types that use changes in intracellular pH for cell signaling or
CC to regulate biochemical or developmental processes.
CC {ECO:0000269|PubMed:29371428}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q80VM9};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed at higher level in stomach, testis and
CC olfactory bulb. {ECO:0000269|PubMed:29371428}.
CC -!- SIMILARITY: Belongs to the otopetrin family. {ECO:0000305}.
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DR EMBL; AB087501; BAC57427.1; -; Genomic_DNA.
DR EMBL; AB087504; BAC67684.1; -; mRNA.
DR EMBL; AK028866; BAC26159.1; -; mRNA.
DR EMBL; AL603828; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK000629; DAA00891.1; -; Genomic_DNA.
DR EMBL; BK000631; DAA00893.1; -; mRNA.
DR EMBL; BK000632; DAA00894.1; -; mRNA.
DR CCDS; CCDS25628.1; -.
DR RefSeq; NP_766389.2; NM_172801.2.
DR RefSeq; XP_006533319.1; XM_006533256.1.
DR AlphaFoldDB; Q80SX5; -.
DR SMR; Q80SX5; -.
DR STRING; 10090.ENSMUSP00000062109; -.
DR PhosphoSitePlus; Q80SX5; -.
DR PaxDb; Q80SX5; -.
DR PRIDE; Q80SX5; -.
DR ProteomicsDB; 294351; -.
DR Antibodypedia; 32060; 95 antibodies from 16 providers.
DR DNASU; 237987; -.
DR Ensembl; ENSMUST00000055490; ENSMUSP00000062109; ENSMUSG00000050201.
DR Ensembl; ENSMUST00000106544; ENSMUSP00000102154; ENSMUSG00000050201.
DR GeneID; 237987; -.
DR KEGG; mmu:237987; -.
DR UCSC; uc007mhb.1; mouse.
DR CTD; 92736; -.
DR MGI; MGI:2388365; Otop2.
DR VEuPathDB; HostDB:ENSMUSG00000050201; -.
DR eggNOG; KOG4740; Eukaryota.
DR GeneTree; ENSGT00940000156691; -.
DR HOGENOM; CLU_032913_0_0_1; -.
DR InParanoid; Q80SX5; -.
DR OMA; IHRTHYS; -.
DR OrthoDB; 524642at2759; -.
DR PhylomeDB; Q80SX5; -.
DR TreeFam; TF313428; -.
DR BioGRID-ORCS; 237987; 3 hits in 72 CRISPR screens.
DR PRO; PR:Q80SX5; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q80SX5; protein.
DR Bgee; ENSMUSG00000050201; Expressed in esophagus and 12 other tissues.
DR Genevisible; Q80SX5; MM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015252; F:proton channel activity; IDA:UniProtKB.
DR GO; GO:1902600; P:proton transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR004878; Otopetrin.
DR PANTHER; PTHR21522; PTHR21522; 1.
DR Pfam; PF03189; Otopetrin; 3.
PE 2: Evidence at transcript level;
KW Cell membrane; Hydrogen ion transport; Ion channel; Ion transport;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..563
FT /note="Proton channel OTOP2"
FT /id="PRO_0000313821"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 528..548
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 198
FT /note="S -> T (in Ref. 3; BAC26159)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 563 AA; 62582 MW; 7F9A28A18ED5D4DE CRC64;
MSEELVPHPN ESLPGPRASP REVWKKGGRL LSVLLAVNVL LLACTLISGG AFNKVAVYDT
DVFALLTTMM LLAALWIVFY LLRTARCPDA VPYRDAHAGP IWLRGGLVLF GICTLVMDVF
KTGYYSSFFE CQSAIKILHP IIQAVFVIVQ TYFLWISAKD CIHTHLDLTR CGLMFTLATN
LAIWMAAVVD ESVHQAHSYS GSHGNTSHTR LNPDSKRAGG AAEEDPCLCS TAICQIFQQG
YFYLYPFNIE YSLFASTMLY VMWKNVGRLL ASTHGHGHTP SRVSLFRETF FAGPVLGLLL
FVVGLAVFIL YEVQVSGERG HTRQALVIYY SFNIVCLGLM TLVSLSGSVI YRFDRRAMDH
HKNPTRTLDV ALLMGAALGQ YAISYYSIVA VVVGSPRDLQ GALNLSHALL MIAQHTFQNV
FIIESLHRGP PGAEPREMPP KEPCQGITFA NLDAIRTLPS CPPTPRLVIP NLESPQEAVA
IISAPRCHWR RRCLKDISLF LLLCNVILWI MPAFGARPHF SNTVEVDFYG YSLWAAIVNI
CLPFGIFYRM HAVSSLLEVY VLS
