OTOP_DROME
ID OTOP_DROME Reviewed; 1576 AA.
AC B7Z0W9; A0A0S0X6R3; B7Z0X0;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 2.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Proton channel OtopLc {ECO:0000305};
DE AltName: Full=Otopetrin-Lc {ECO:0000303|PubMed:29371428};
DE Short=DmOtopLc {ECO:0000303|PubMed:29371428};
GN Name=OtopLc {ECO:0000303|PubMed:29371428, ECO:0000312|FlyBase:FBgn0259150};
GN ORFNames=CG42265 {ECO:0000312|FlyBase:FBgn0259150};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP FUNCTION.
RX PubMed=29371428; DOI=10.1126/science.aao3264;
RA Tu Y.H., Cooper A.J., Teng B., Chang R.B., Artiga D.J., Turner H.N.,
RA Mulhall E.M., Ye W., Smith A.D., Liman E.R.;
RT "An evolutionarily conserved gene family encodes proton-selective ion
RT channels.";
RL Science 359:1047-1050(2018).
CC -!- FUNCTION: Proton-selective channel that specifically transports protons
CC into cells. Proton-selective channel activity is probably required in
CC cell types that use changes in intracellular pH for cell signaling or
CC to regulate biochemical or developmental processes.
CC {ECO:0000269|PubMed:29371428}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q80VM9};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=D;
CC IsoId=B7Z0W9-1; Sequence=Displayed;
CC Name=E;
CC IsoId=B7Z0W9-2; Sequence=VSP_059520;
CC Name=F;
CC IsoId=B7Z0W9-3; Sequence=VSP_059521;
CC -!- SIMILARITY: Belongs to the otopetrin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014298; ACL82892.2; -; Genomic_DNA.
DR EMBL; AE014298; ACL82893.2; -; Genomic_DNA.
DR EMBL; AE014298; ALI51143.1; -; Genomic_DNA.
DR RefSeq; NP_001138160.2; NM_001144688.3. [B7Z0W9-1]
DR RefSeq; NP_001138161.2; NM_001144689.3.
DR RefSeq; NP_001303575.1; NM_001316646.1.
DR STRING; 7227.FBpp0303595; -.
DR GlyGen; B7Z0W9; 4 sites.
DR PRIDE; B7Z0W9; -.
DR EnsemblMetazoa; FBtr0331167; FBpp0303594; FBgn0259150. [B7Z0W9-1]
DR EnsemblMetazoa; FBtr0331168; FBpp0303595; FBgn0259150.
DR EnsemblMetazoa; FBtr0346834; FBpp0312397; FBgn0259150.
DR GeneID; 7354434; -.
DR KEGG; dme:Dmel_CG42265; -.
DR CTD; 7354434; -.
DR FlyBase; FBgn0259150; OtopLc.
DR VEuPathDB; VectorBase:FBgn0259150; -.
DR eggNOG; KOG4740; Eukaryota.
DR HOGENOM; CLU_244473_0_0_1; -.
DR InParanoid; B7Z0W9; -.
DR BioGRID-ORCS; 7354434; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 7354434; -.
DR PRO; PR:B7Z0W9; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0259150; Expressed in brain and 5 other tissues.
DR ExpressionAtlas; B7Z0W9; baseline and differential.
DR Genevisible; B7Z0W9; DM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015252; F:proton channel activity; IDA:UniProtKB.
DR GO; GO:1902600; P:proton transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR004878; Otopetrin.
DR PANTHER; PTHR21522; PTHR21522; 1.
DR Pfam; PF03189; Otopetrin; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Cell membrane; Glycoprotein; Hydrogen ion transport;
KW Ion channel; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..1576
FT /note="Proton channel OtopLc"
FT /id="PRO_0000443805"
FT TRANSMEM 741..761
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 891..911
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 931..951
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1019..1039
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1051..1071
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1084..1104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1179..1199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1239..1259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1272..1292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1310..1330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1340..1360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1381..1401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1412..1432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..740
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 771..845
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 966..1001
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1497..1551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 157..171
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..217
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 249..268
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..298
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..339
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..357
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 358..376
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 402..422
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 433..460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 471..555
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 565..579
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 666..684
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 685..708
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 717..731
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 773..799
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 830..845
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 230
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 267
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1121
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1479
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1457..1576
FT /note="Missing (in isoform E)"
FT /id="VSP_059520"
FT VAR_SEQ 1576
FT /note="E -> EVYAHFEFIDAISGHELSFV (in isoform F)"
FT /id="VSP_059521"
SQ SEQUENCE 1576 AA; 169751 MW; 3325AE36884ABBB7 CRC64;
MDSSPDLSLK LRRGSSDSRD NFYMDFAQGI DSDIEEVDNT ANNQEAGEVP PPPLPTVSLA
EEVLLLVAPP PPPPSLLGQP LPTLTETDDI PPTPTPPPQQ KDDEGDDEDE REEPVPEQDQ
GAPAAPSPPG SPINSVLELE LIPPPPLSPM DDAGLRTDDD GEGEETDDAE EVAAIPPPHE
MLDIESNPDE EEEEEEQEQA SQEDTPKEED EEEDDDKSTP PPPLPPLPSN FSYVQGHNLG
QVTPPLTKSP SNSPSPPVTP PPCPELNISR MVSPPAQHIS QIPPLTPSDE SEGEAESQPN
SPPLRLDAEQ PPPDMDQPEP EDQPPEPENE PEPEPEPEPE PEPVSGARED YSRSLDNEDE
STTITTPPSN GYSASSIIAP PPEHFAELDE DRGFIPPPPL EQEPEEEVEE EEEEEEEELT
KETDEISVDR ESLQDQGGDS ISSPRPASIL TGSISTSVGG GAGGSPKPES RGPSRSGSQR
SQLRSGSQQG SIAESRGGSR IGSRTGSVAS AQAAGVLSPQ ASLKSQTSIR SQGQAGVRSP
AGSIKSGSQR MQSPQAGEGA PAMPSPPLMR SPPPELARQM HSPPRITTPP RVCSPPLVSS
PPKLAESAAA AVGVAATVKE QIGSSSSTAE PLEPSKPEPL KPPIATVSYQ DEQKPSPPPT
AAAAPAVTTT AATTAVTSQP RSHFTSSHHH YHLPHQFQHP HHQNHHTHSV RVPTPTVPSS
YAPPPPPDSG SSSSPVDRRR LFMAGVAPPI AAGAGSLMAM PAEPAVAISP GRVSARSGSQ
HHVTIDESSL PSHKGNIQET PGPSGLIIGG GDGDGDRDIG GGGGPDSSDP PSSPGGSSSQ
PALSGSQADG QLALMYHSHQ LTNYPVLPAI KRTHRPSFVY PPMPRVKAGD ALATLFSALY
GKLLVVMGIA FPMAEVISTY IPPSFYEVYY LYLYIGSMIF LLFMYATLIW GRPKLPVPIA
SPSKSATKAS GTDSMDESDT DSNSVHHRLP PPIPVRRPSL LSPLGRRDAH YGSFYLRMGA
VAFGIGSMIY SGLEFGQYFE LNPDTKCHNV LLALTPATRM AFIFIQMYFI FLNNEQIKVY
RYKIIARFGL MHMIGTNLAV WLNVLIQETK HEILTFYNPE NRTLRISHRI PGHSRGHAII
QHDPTAHLRV PRGLKGPYQI FECRRTNIIG TLVQDASPFL FPCTIEYSLI CAAILYVMWR
SISRPQTPTP QRPDMISSPM KRSPHHYSVD CARAHKGLFV GILILVLTII SLIIFFVLIS
RPEFVAMAVT EVTICELLIY GTATIATLVG MIQIRHLQYD AYRSFSLDDI LLVGAQTGSF
LYNIFTVIAG HFTLRSDDML VPINALASIV QTACQTMFIL DASRRQAVSP EHLRKKPGRE
IVTFMLVVNL AMWAISTLEK SRAESHPIQL NFYGLWAWTI ITHVSMPLAI FYRFHSTVCL
CEIWKRAYKL KPTYMXEFAR SRIQSIAQQQ QFCEDLKTNL SYCYCSTTLA GGELETVEEV
DSGESNSAED AGAGAGSGGS RGSGGGAGAA EAGEAGEEGQ QGGDSSCGLK APIRALSPQS
LNTEKAFCPV YVINGE
