ID   ASCL2_RAT               Reviewed;         260 AA.
AC   P19360;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 144.
DE   RecName: Full=Achaete-scute homolog 2;
DE   AltName: Full=Mash2;
GN   Name=Ascl2; Synonyms=Ash2, Mash-2, Mash2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RX   PubMed=2392153; DOI=10.1038/346858a0;
RA   Johnson J.E., Birren S.J., Anderson D.J.;
RT   "Two rat homologues of Drosophila achaete-scute specifically expressed in
RT   neuronal precursors.";
RL   Nature 346:858-861(1990).
CC   -!- FUNCTION: AS-C proteins are involved in the determination of the
CC       neuronal precursors in the peripheral nervous system and the central
CC       nervous system.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein. Part of a complex composed at least of ASCL2, EMSY, HCFC1,
CC       HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this
CC       complex may have a histone H3-specific methyltransferase activity (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Neuronal precursor cells.
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DR   EMBL; X53724; CAA37759.1; -; mRNA.
DR   PIR; S11562; S11562.
DR   RefSeq; NP_113691.1; NM_031503.2.
DR   AlphaFoldDB; P19360; -.
DR   SMR; P19360; -.
DR   STRING; 10116.ENSRNOP00000027688; -.
DR   PaxDb; P19360; -.
DR   PRIDE; P19360; -.
DR   Ensembl; ENSRNOT00000027688; ENSRNOP00000027688; ENSRNOG00000020434.
DR   GeneID; 24209; -.
DR   KEGG; rno:24209; -.
DR   UCSC; RGD:2159; rat.
DR   CTD; 430; -.
DR   RGD; 2159; Ascl2.
DR   eggNOG; KOG4029; Eukaryota.
DR   GeneTree; ENSGT00940000163041; -.
DR   HOGENOM; CLU_063523_3_0_1; -.
DR   InParanoid; P19360; -.
DR   OMA; ACPRESC; -.
DR   OrthoDB; 1131543at2759; -.
DR   PhylomeDB; P19360; -.
DR   TreeFam; TF322889; -.
DR   PRO; PR:P19360; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000020434; Expressed in jejunum and 10 other tissues.
DR   Genevisible; P19360; RN.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; IDA:RGD.
DR   GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:RGD.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:RGD.
DR   GO; GO:0070888; F:E-box binding; ISO:RGD.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR   GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR   GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR   GO; GO:0010626; P:negative regulation of Schwann cell proliferation; IDA:RGD.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0001890; P:placenta development; ISO:RGD.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR   GO; GO:0001666; P:response to hypoxia; ISO:RGD.
DR   GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR   GO; GO:0035019; P:somatic stem cell population maintenance; ISO:RGD.
DR   GO; GO:0060708; P:spongiotrophoblast differentiation; ISO:RGD.
DR   GO; GO:0060712; P:spongiotrophoblast layer development; ISO:RGD.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR015660; MASH1/Ascl1a-like.
DR   PANTHER; PTHR13935; PTHR13935; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..260
FT                   /note="Achaete-scute homolog 2"
FT                   /id="PRO_0000127132"
FT   DOMAIN          118..170
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          85..126
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          191..239
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        194..239
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   260 AA;  27695 MW;  E10402DD1F7434C5 CRC64;
     MESHFNWYGV PRLQKASDAC PRESCSSALP EAREGANVHF PPHPVPREHF SCGAPKPVAG
     APALNASLMD GGALPRLVPT SSGVAGACTA RRRPPSPELL RCSRRRRSGA TEASSSSAAV
     ARRNERERNR VKLVNLGFQA LRQHVPHGGA NKKLSKVETL RSAVEYIRAL QRLLAEHDAV
     RAALSGGLLT PATRPSDVCT QPSASPASAS LSCTSTSPDR LGCSEPASPR SAYSSEDSSC
     EGETYPMGQM FDFSNWLGGY