OTP_DROME
ID OTP_DROME Reviewed; 409 AA.
AC P56672; A4IJ64; Q3KN52; Q7KVQ3; Q9W2Q4;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Homeobox protein orthopedia;
GN Name=otp; ORFNames=CG10036;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM C), FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=7913821; DOI=10.1016/0896-6273(94)90461-8;
RA Simeone A., D'Apice M.R., Nigro V., Casanova J., Graziani F., Acampora D.,
RA Avantaggiato V.;
RT "Orthopedia, a novel homeobox-containing gene expressed in the developing
RT CNS of both mouse and Drosophila.";
RL Neuron 13:83-101(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS C AND E).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B.,
RA Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in the development of the central nervous
CC system. {ECO:0000269|PubMed:7913821}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00138}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=E;
CC IsoId=P56672-5; Sequence=Displayed;
CC Name=C;
CC IsoId=P56672-3; Sequence=VSP_026420, VSP_036997, VSP_036998;
CC -!- TISSUE SPECIFICITY: First appears at gastrulation in the ectodermal
CC proctodeum and later in the hindgut, anal plate, and along the CNS.
CC {ECO:0000269|PubMed:7913821}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 is the initiator or if the
CC sequence starts further upstream. {ECO:0000305}.
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DR EMBL; AE013599; AAS64751.3; -; Genomic_DNA.
DR EMBL; AE013599; ABI31108.1; -; Genomic_DNA.
DR EMBL; BT023887; ABA81821.1; -; mRNA.
DR EMBL; BT030422; ABO52842.1; -; mRNA.
DR RefSeq; NP_001036563.1; NM_001043098.2. [P56672-3]
DR RefSeq; NP_001097388.2; NM_001103918.3. [P56672-5]
DR AlphaFoldDB; P56672; -.
DR SMR; P56672; -.
DR BioGRID; 63008; 18.
DR DIP; DIP-17198N; -.
DR IntAct; P56672; 40.
DR STRING; 7227.FBpp0288728; -.
DR PaxDb; P56672; -.
DR DNASU; 37364; -.
DR EnsemblMetazoa; FBtr0110772; FBpp0110069; FBgn0015524. [P56672-3]
DR EnsemblMetazoa; FBtr0290289; FBpp0288728; FBgn0015524. [P56672-5]
DR GeneID; 37364; -.
DR KEGG; dme:Dmel_CG10036; -.
DR UCSC; CG10036-RC; d. melanogaster. [P56672-5]
DR CTD; 23440; -.
DR FlyBase; FBgn0015524; otp.
DR VEuPathDB; VectorBase:FBgn0015524; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000170167; -.
DR InParanoid; P56672; -.
DR PhylomeDB; P56672; -.
DR BioGRID-ORCS; 37364; 0 hits in 1 CRISPR screen.
DR ChiTaRS; otp; fly.
DR GenomeRNAi; 37364; -.
DR PRO; PR:P56672; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0015524; Expressed in embryonic large intestine (Drosophila) and 18 other tissues.
DR ExpressionAtlas; P56672; baseline and differential.
DR Genevisible; P56672; DM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome.
FT CHAIN 1..409
FT /note="Homeobox protein orthopedia"
FT /id="PRO_0000049206"
FT DNA_BIND 109..168
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 81..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 277..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 372..385
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT COMPBIAS 81..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..96
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_026420"
FT VAR_SEQ 97..108
FT /note="NGNNNNSMQQQD -> MQQQDQHLDKNK (in isoform C)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_036997"
FT VAR_SEQ 223..264
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_036998"
SQ SEQUENCE 409 AA; 43173 MW; E186EFAC8C125BE9 CRC64;
MLNNLGANVL GPKDCDLPKA ALTALHAGVN SFGQLPVGPN LADLGGGAGG GGSSGGVPVG
GGVARLHISG GLCDNSNALN GGNGSSGNGN GNNNNGNGNN NNSMQQQDQK RHRTRFTPAQ
LNELERCFSK THYPDIFMRE EIAMRIGLTE SRVQVWFQNR RAKWKKRKKT TNVFRTPGAL
LPSHGLPPFG ANITNIAMGD GLCGTGMFGG DRWSVGVNPM TAGFGQLNQS SPLSSSLNSG
LNSGINMGSA LGAGSYQHYG LNALGDSMMY QHSVGGVSCG PSGSPSATTP PNMNSCSSVT
PPPLSAQPNS SQNELNGEPM PLHQQQQQQT HQHQQQQTHQ HHPMAPPTPT QQQQQLPQSM
QSPSDGANDT LHSIAALRRR ASELNAIPSY LQMAPHNYEH YNSNSNSVY
