OTP_MOUSE
ID OTP_MOUSE Reviewed; 325 AA.
AC O09113;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Homeobox protein orthopedia;
GN Name=Otp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND
RP FUNCTION.
RC STRAIN=BALB/cJ; TISSUE=Embryo;
RX PubMed=7913821; DOI=10.1016/0896-6273(94)90461-8;
RA Simeone A., D'Apice M.R., Nigro V., Casanova J., Graziani F., Acampora D.,
RA Avantaggiato V.;
RT "Orthopedia, a novel homeobox-containing gene expressed in the developing
RT CNS of both mouse and Drosophila.";
RL Neuron 13:83-101(1994).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10557207; DOI=10.1101/gad.13.21.2787;
RA Acampora D., Postiglione M.P., Avantaggiato V., Di Bonito M.,
RA Vaccarino F.M., Michaud J., Simeone A.;
RT "Progressive impairment of developing neuroendocrine cell lineages in the
RT hypothalamus of mice lacking the Orthopedia gene.";
RL Genes Dev. 13:2787-2800(1999).
RN [3]
RP FUNCTION.
RX PubMed=11071765; DOI=10.1006/dbio.2000.9902;
RA Wang W., Lufkin T.;
RT "The murine Otp homeobox gene plays an essential role in the specification
RT of neuronal cell lineages in the developing hypothalamus.";
RL Dev. Biol. 227:432-449(2000).
RN [4]
RP FUNCTION.
RX PubMed=17481897; DOI=10.1016/j.cub.2007.04.003;
RA Ryu S., Mahler J., Acampora D., Holzschuh J., Erhardt S., Omodei D.,
RA Simeone A., Driever W.;
RT "Orthopedia homeodomain protein is essential for diencephalic dopaminergic
RT neuron development.";
RL Curr. Biol. 17:873-880(2007).
CC -!- FUNCTION: Involved in the specification of hypothalamic neuroendocrine
CC cells. Specifically required for the specification of diencephalic
CC dopaminergic neurons of the A11 group. {ECO:0000269|PubMed:10557207,
CC ECO:0000269|PubMed:11071765, ECO:0000269|PubMed:17481897,
CC ECO:0000269|PubMed:7913821}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00138}.
CC -!- TISSUE SPECIFICITY: Restricted regions of the developing forebrain,
CC hindbrain, and spinal cord. {ECO:0000269|PubMed:7913821}.
CC -!- DEVELOPMENTAL STAGE: First detected at 9.5 dpc in restricted domains of
CC the developing diencephalon and along all the hindbrain and the spinal
CC cord. At 10 dpc, found in the medioventral region of the developing
CC spinal cord. At 12.5 dpc, expressed in restricted zones in the
CC preoptic, postoptic and dorsoposterior regions. At later stages, in the
CC intermedial region of the lateral horn, in the optic tract, the
CC presumptive stria terminalis, the amygdaloid complex of the lateral
CC horn in the spinal cord. {ECO:0000269|PubMed:7913821}.
CC -!- DISRUPTION PHENOTYPE: Death at birth or within the first 2 postnatal
CC days due to defects in neuroendocrine hypothalamus differentiation.
CC {ECO:0000269|PubMed:10557207}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; Y10413; CAA71439.1; -; mRNA.
DR CCDS; CCDS26695.1; -.
DR RefSeq; NP_035151.1; NM_011021.3.
DR AlphaFoldDB; O09113; -.
DR SMR; O09113; -.
DR STRING; 10090.ENSMUSP00000022195; -.
DR PhosphoSitePlus; O09113; -.
DR MaxQB; O09113; -.
DR PaxDb; O09113; -.
DR PRIDE; O09113; -.
DR ProteomicsDB; 294132; -.
DR Antibodypedia; 12584; 140 antibodies from 23 providers.
DR DNASU; 18420; -.
DR Ensembl; ENSMUST00000022195; ENSMUSP00000022195; ENSMUSG00000021685.
DR GeneID; 18420; -.
DR KEGG; mmu:18420; -.
DR UCSC; uc007rly.1; mouse.
DR CTD; 23440; -.
DR MGI; MGI:99835; Otp.
DR VEuPathDB; HostDB:ENSMUSG00000021685; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000159952; -.
DR HOGENOM; CLU_056068_0_0_1; -.
DR InParanoid; O09113; -.
DR OMA; MLPGEDI; -.
DR OrthoDB; 1243020at2759; -.
DR PhylomeDB; O09113; -.
DR TreeFam; TF351614; -.
DR BioGRID-ORCS; 18420; 1 hit in 72 CRISPR screens.
DR PRO; PR:O09113; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; O09113; protein.
DR Bgee; ENSMUSG00000021685; Expressed in median eminence of neurohypophysis and 89 other tissues.
DR Genevisible; O09113; MM.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:MGI.
DR GO; GO:0021879; P:forebrain neuron differentiation; IMP:MGI.
DR GO; GO:0021979; P:hypothalamus cell differentiation; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0007405; P:neuroblast proliferation; IMP:MGI.
DR GO; GO:0061101; P:neuroendocrine cell differentiation; IMP:MGI.
DR GO; GO:0021985; P:neurohypophysis development; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..325
FT /note="Homeobox protein orthopedia"
FT /id="PRO_0000049204"
FT DNA_BIND 104..163
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 32..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 306..319
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT COMPBIAS 88..102
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 325 AA; 34159 MW; 429E3A91E0A9BA5F CRC64;
MLSHADLLDA RLGMKDAAEL LGHREAVKCR LGVGGSDPGG HPGDLAPNSD PVEGATLLPG
EDITTVGSTP ASLAVSAKDP DKQPGPQGGP NPSQAGQQQG QQKQKRHRTR FTPAQLNELE
RSFAKTHYPD IFMREELALR IGLTESRVQV WFQNRRAKWK KRKKTTNVFR APGTLLPTPG
LPQFPSAAAA AAAAMGDSLC SFHANDTRWA AAAMPGVSQL PLPPALGRQQ AMAQSLSQCS
LAAGPPPNSM GLSNSLAGSN GAGLQSHLYQ PAFPGMVPAS LPGPSNVSGS PQLCSSPDSS
DVWRGTSIAS LRRKALEHTV SMSFT
