ID   OTRA_STRRM              Reviewed;         663 AA.
AC   Q55002;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Oxytetracycline resistance protein;
GN   Name=otrA;
OS   Streptomyces rimosus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1927;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=15883R;
RX   PubMed=1809836; DOI=10.1111/j.1365-2958.1991.tb01852.x;
RA   Doyle D., McDowall K.J., Butler M.J., Hunter I.S.;
RT   "Characterization of an oxytetracycline-resistance gene, otrA, of
RT   Streptomyces rimosus.";
RL   Mol. Microbiol. 5:2923-2933(1991).
CC   -!- FUNCTION: Abolishes the inhibitory effect of oxytetracycline on protein
CC       synthesis by a non-covalent modification of the ribosomes.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. TetM/TetO
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU01059}.
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DR   EMBL; X53401; CAA37477.1; -; Genomic_DNA.
DR   PIR; S18572; S18572.
DR   RefSeq; WP_063854497.1; NG_048026.1.
DR   AlphaFoldDB; Q55002; -.
DR   SMR; Q55002; -.
DR   KEGG; ag:CAA37477; -.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   CDD; cd03711; Tet_C; 1.
DR   Gene3D; 3.30.230.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR035647; EFG_III/V.
DR   InterPro; IPR000640; EFG_V-like.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR   InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR035650; Tet_C.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR005517; Transl_elong_EFG/EF2_IV.
DR   Pfam; PF00679; EFG_C; 1.
DR   Pfam; PF03764; EFG_IV; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54211; SSF54211; 1.
DR   SUPFAM; SSF54980; SSF54980; 2.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; GTP-binding; Nucleotide-binding;
KW   Protein biosynthesis.
FT   CHAIN           1..663
FT                   /note="Oxytetracycline resistance protein"
FT                   /id="PRO_0000091517"
FT   DOMAIN          1..252
FT                   /note="tr-type G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT   BINDING         10..17
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         74..78
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         128..131
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   663 AA;  71786 MW;  FEB03DE38856194D CRC64;
     MNKLNLGILA HVDAGKTSLT ERLLHRTGVI DEVGSVDAGT TTTDSMELER QRGITIRSAV
     ATFVLDDLKV NLIDTPGHSD FISEVERALG VLDGAVLVVS AVEGVQPQTR ILMRTLRRLG
     IPTLVFVNKI DRGGARPDGV LREIRDRLTP AAVALSAVAD AGTPRARAIA LGPDTDPDFA
     VRVGELLADH DDAFLTAYLD EEHVLTEKEY AEELAAQTAR GLVHPVYFGS ALTGEGLDHL
     VHGIRELLPS VHASQDAPLR ATVFKVDRGA RGEAVAYLRL VSGTLGTRDS VTLHRVDHTG
     RVTEHAGRIT ALRVFEHGSA TSETRATAGD IAQAWGLKDV RVGDRAGHLD GPPPRNFFAP
     PSLETVIRPE RPEEAGRLHA ALRMLDEQDP SIDLRQDEEN AAGAVVRLYG EVQKEILGST
     LAESFGVRVR FDPTRTVCIE KPVGTGEALI ELDTRTHNYF WGAPWVCASD RPSPARAITF
     RLAVELGSLP LAFHKAIEET VHTTLRHGLY GWQVTDCAVT LTRTGVRSPV SAADDFRKAN
     ARLVLMDALG RAGTEVHEPV SSFELEVPAA RLSPVLAKLA ELGATPGVPT AEGDVFRLEG
     TMPTSLVHDF NQRVPGLTQG EGVFLAEHRG YRPAVGQPPV RPRPEGPNPL NRDEYILHVL
     KRV