OTSB_SALTS
ID OTSB_SALTS Reviewed; 267 AA.
AC E1WGG9; Q9L894;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Trehalose-phosphate phosphatase;
DE Short=TPP;
DE EC=3.1.3.12;
DE AltName: Full=Trehalose 6-phosphate phosphatase;
DE AltName: Full=Trehalose-phosphatase;
GN Name=otsB; OrderedLocusNames=SL1344_1863;
OS Salmonella typhimurium (strain SL1344).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=216597;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SL1344;
RA Howells A.M., Bullifent H.L., Dhaliwal K., Griffin K., Frith G.,
RA Tunnacliffe A., Titball R.W.;
RT "Trehalose biosynthesis operon in S. typhimurium.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SL1344;
RX PubMed=22538806; DOI=10.1073/pnas.1201061109;
RA Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K.,
RA Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A.,
RA Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S.,
RA Ussery D.W., Dorman C.J., Thomson N.R., Vogel J., Hinton J.C.;
RT "The transcriptional landscape and small RNAs of Salmonella enterica
RT serovar Typhimurium.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012).
CC -!- FUNCTION: Removes the phosphate from trehalose 6-phosphate to produce
CC free trehalose. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-
CC trehalose + phosphate; Xref=Rhea:RHEA:23420, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:16551, ChEBI:CHEBI:43474, ChEBI:CHEBI:58429; EC=3.1.3.12;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC -!- SIMILARITY: Belongs to the trehalose phosphatase family. {ECO:0000305}.
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DR EMBL; AF213176; AAF34581.1; -; Genomic_DNA.
DR EMBL; FQ312003; CBW17957.1; -; Genomic_DNA.
DR RefSeq; WP_000830110.1; NZ_QASL01000015.1.
DR PDB; 6UPB; X-ray; 1.89 A; A/B=1-267.
DR PDB; 6UPC; X-ray; 2.50 A; A/B=1-267.
DR PDB; 6UPD; X-ray; 2.05 A; A/B=1-267.
DR PDB; 6UPE; X-ray; 2.24 A; A/B=1-267.
DR PDBsum; 6UPB; -.
DR PDBsum; 6UPC; -.
DR PDBsum; 6UPD; -.
DR PDBsum; 6UPE; -.
DR AlphaFoldDB; E1WGG9; -.
DR SMR; E1WGG9; -.
DR EnsemblBacteria; CBW17957; CBW17957; SL1344_1863.
DR KEGG; sey:SL1344_1863; -.
DR PATRIC; fig|216597.6.peg.2068; -.
DR HOGENOM; CLU_037265_2_0_6; -.
DR OMA; YGAERWD; -.
DR BioCyc; SENT216597:SL1344_RS09650-MON; -.
DR UniPathway; UPA00299; -.
DR Proteomes; UP000008962; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProt.
DR GO; GO:0004805; F:trehalose-phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR044651; OTSB-like.
DR InterPro; IPR003337; Trehalose_PPase.
DR PANTHER; PTHR43768; PTHR43768; 1.
DR Pfam; PF02358; Trehalose_PPase; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR00685; T6PP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Metal-binding.
FT CHAIN 1..267
FT /note="Trehalose-phosphate phosphatase"
FT /id="PRO_0000406086"
FT ACT_SITE 20
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 20..22
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 20
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 22
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 198
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT STRAND 13..19
FT /evidence="ECO:0007829|PDB:6UPB"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 32..34
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 39..50
FT /evidence="ECO:0007829|PDB:6UPB"
FT TURN 51..54
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 56..59
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 64..71
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 100..115
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 121..124
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 137..139
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 140..153
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 157..162
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 165..170
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 175..183
FT /evidence="ECO:0007829|PDB:6UPB"
FT TURN 186..190
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 191..197
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 200..211
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 215..222
FT /evidence="ECO:0007829|PDB:6UPB"
FT STRAND 227..232
FT /evidence="ECO:0007829|PDB:6UPB"
FT HELIX 233..244
FT /evidence="ECO:0007829|PDB:6UPB"
SQ SEQUENCE 267 AA; 29261 MW; 8AD6FB1F3799F797 CRC64;
MAEPLTVSPE LTANYAYFFD LDGTLAEIKP HPDQVVVPHK ILQLLDRLAA HNAGALALIS
GRSMTELDAL AKPFRFPLAG VHGAERRDIN GKTHIVRLPE AVVREVEALL RSTLVALPGT
ELESKGMAFA LHYRQAPEHE AALLALAQHV TQHWPQLALQ LGKCVVEIKP KGTNKGEAIA
AFMQEAPFAG RIPVFVGDDL TDEAGFGVVN HAGGISVKVG VGATQAAWRL ESVPDVWRWL
EQINYPQQEQ QVMNNRRDGY ESFSRSI
