ID   OTSB_SINFN              Reviewed;         265 AA.
AC   P55611;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Probable trehalose-phosphate phosphatase;
DE            Short=TPP;
DE            EC=3.1.3.12;
DE   AltName: Full=Trehalose 6-phosphate phosphatase;
DE   AltName: Full=Trehalose-phosphatase;
GN   Name=otsB; OrderedLocusNames=NGR_a02100; ORFNames=y4pB;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Removes the phosphate from trehalose 6-phosphate to produce
CC       free trehalose. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-
CC         trehalose + phosphate; Xref=Rhea:RHEA:23420, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:16551, ChEBI:CHEBI:43474, ChEBI:CHEBI:58429; EC=3.1.3.12;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC   -!- SIMILARITY: Belongs to the trehalose phosphatase family. {ECO:0000305}.
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DR   EMBL; U00090; AAB91812.1; -; Genomic_DNA.
DR   RefSeq; NP_444015.1; NC_000914.2.
DR   RefSeq; WP_010875237.1; NC_000914.2.
DR   AlphaFoldDB; P55611; -.
DR   SMR; P55611; -.
DR   STRING; 394.NGR_a02100; -.
DR   EnsemblBacteria; AAB91812; AAB91812; NGR_a02100.
DR   KEGG; rhi:NGR_a02100; -.
DR   PATRIC; fig|394.7.peg.221; -.
DR   eggNOG; COG1877; Bacteria.
DR   HOGENOM; CLU_037265_2_0_5; -.
DR   OMA; YGAERWD; -.
DR   OrthoDB; 1374424at2; -.
DR   UniPathway; UPA00299; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004805; F:trehalose-phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006379; HAD-SF_hydro_IIB.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR044651; OTSB-like.
DR   InterPro; IPR003337; Trehalose_PPase.
DR   PANTHER; PTHR43768; PTHR43768; 1.
DR   Pfam; PF02358; Trehalose_PPase; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR   TIGRFAMs; TIGR00685; T6PP; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Plasmid; Reference proteome.
FT   CHAIN           1..265
FT                   /note="Probable trehalose-phosphate phosphatase"
FT                   /id="PRO_0000058101"
FT   ACT_SITE        35
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         35..37
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         35
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         37
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         213
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   265 AA;  27958 MW;  AD21CF43EF7D794A CRC64;
     MSCQTQLLSA SEAALGEDFL SALSTDLDNW ALFLDIDGTL LDLAETPDAV AVPPSLPASL
     DHLSKKLGGA LALVTGRGLD YADQLFSPAN FPIAGLHGAE RRDPDGRVHK AAETADFERL
     KAELVAATAS WAGVLIEDKG AAVAAHYRLA PDRQLELEQL MEWALYRAGP DWAIQHGKMV
     VEIRPARANK GDAVAAFLGQ PPFAGRRAIA IGDDVTDEAM FRTVNRLGGL SIRIGPPVPA
     SEALGSIPSA EALRGIIAAL ALLNI