位置:首页 > 蛋白库 > OTUB1_RAT
OTUB1_RAT
ID   OTUB1_RAT               Reviewed;         271 AA.
AC   B2RYG6;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Ubiquitin thioesterase OTUB1 {ECO:0000250|UniProtKB:Q7TQI3};
DE            EC=3.4.19.12 {ECO:0000250|UniProtKB:Q96FW1};
DE   AltName: Full=Deubiquitinating enzyme OTUB1 {ECO:0000250|UniProtKB:Q7TQI3};
DE   AltName: Full=OTU domain-containing ubiquitin aldehyde-binding protein 1 {ECO:0000250|UniProtKB:Q7TQI3};
DE   AltName: Full=Otubain-1 {ECO:0000250|UniProtKB:Q7TQI3};
DE   AltName: Full=Ubiquitin-specific-processing protease OTUB1 {ECO:0000250|UniProtKB:Q7TQI3};
GN   Name=Otub1 {ECO:0000312|EMBL:AAI66771.1};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:EDM12666.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EMBL:AAI66771.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate {ECO:0000312|EMBL:AAI66771.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=19343716; DOI=10.1002/pmic.200800664;
RA   Maurya D.K., Sundaram C.S., Bhargava P.;
RT   "Proteome profile of the mature rat olfactory bulb.";
RL   Proteomics 9:2593-2599(2009).
CC   -!- FUNCTION: Hydrolase that can specifically remove compared to 'Lys-48'-
CC       linked conjugated ubiquitin from proteins and plays an important
CC       regulatory role at the level of protein turnover by preventing
CC       degradation. Regulator of T-cell anergy, a phenomenon that occurs when
CC       T-cells are rendered unresponsive to antigen rechallenge and no longer
CC       respond to their cognate antigen. Acts via its interaction with
CC       RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated
CC       ubiquitination, but does not deubiquitinate polyubiquitinated RNF128.
CC       Deubiquitinates estrogen receptor alpha (ESR1). Mediates
CC       deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-
CC       63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also
CC       capable of removing NEDD8 from NEDD8 conjugates, but with a much lower
CC       preference compared to 'Lys-48'-linked ubiquitin (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: Plays a key non-catalytic role in DNA repair regulation by
CC       inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that
CC       promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA
CC       damage sites. Inhibits RNF168 independently of ubiquitin thioesterase
CC       activity by binding and inhibiting UBE2N/UBC13, the E2 partner of
CC       RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and
CC       H2AX marks. Inhibition occurs by binding to free ubiquitin: free
CC       ubiquitin acts as an allosteric regulator that increases affinity for
CC       UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-
CC       UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a
CC       cleaved 'Lys48'-linked di-ubiquitin chain (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q96FW1};
CC   -!- ACTIVITY REGULATION: By free ubiquitin: binding of free ubiquitin
CC       triggers conformational changes in the OTU domain and formation of a
CC       ubiquitin-binding helix in the N-terminus, promoting binding of the
CC       conjugated donor ubiquitin in UBE2N/UBC13 to OTUB1.
CC   -!- SUBUNIT: Interacts with RNF128. Forms a ternary complex with RNF128 and
CC       USP8. Interacts with FUS, ESR1 and RACK1. Interacts with UBE2N/UBC13
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19343716}.
CC   -!- SIMILARITY: Belongs to the peptidase C65 family. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CH473953; EDM12666.1; -; Genomic_DNA.
DR   EMBL; BC166771; AAI66771.1; -; mRNA.
DR   RefSeq; NP_001099802.1; NM_001106332.1.
DR   AlphaFoldDB; B2RYG6; -.
DR   SMR; B2RYG6; -.
DR   BioGRID; 254414; 3.
DR   IntAct; B2RYG6; 2.
DR   MINT; B2RYG6; -.
DR   STRING; 10116.ENSRNOP00000028752; -.
DR   iPTMnet; B2RYG6; -.
DR   PhosphoSitePlus; B2RYG6; -.
DR   SwissPalm; B2RYG6; -.
DR   World-2DPAGE; 0004:B2RYG6; -.
DR   jPOST; B2RYG6; -.
DR   PaxDb; B2RYG6; -.
DR   PeptideAtlas; B2RYG6; -.
DR   PRIDE; B2RYG6; -.
DR   Ensembl; ENSRNOT00000101186; ENSRNOP00000088887; ENSRNOG00000021175.
DR   GeneID; 293705; -.
DR   KEGG; rno:293705; -.
DR   UCSC; RGD:1311329; rat.
DR   CTD; 55611; -.
DR   RGD; 1311329; Otub1.
DR   eggNOG; KOG3991; Eukaryota.
DR   GeneTree; ENSGT00390000006979; -.
DR   HOGENOM; CLU_014832_3_0_1; -.
DR   InParanoid; B2RYG6; -.
DR   OMA; VRVRYMD; -.
DR   OrthoDB; 1257066at2759; -.
DR   PhylomeDB; B2RYG6; -.
DR   TreeFam; TF314145; -.
DR   Reactome; R-RNO-5689880; Ub-specific processing proteases.
DR   Reactome; R-RNO-5689896; Ovarian tumor domain proteases.
DR   PRO; PR:B2RYG6; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Proteomes; UP000234681; Chromosome 1.
DR   Bgee; ENSRNOG00000021175; Expressed in frontal cortex and 19 other tissues.
DR   Genevisible; B2RYG6; RN.
DR   GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR   GO; GO:0005634; C:nucleus; IDA:RGD.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; ISS:UniProtKB.
DR   GO; GO:0019784; F:deNEDDylase activity; ISS:UniProtKB.
DR   GO; GO:0043130; F:ubiquitin binding; ISS:UniProtKB.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:RGD.
DR   GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR   GO; GO:0071347; P:cellular response to interleukin-1; IEP:RGD.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:2000780; P:negative regulation of double-strand break repair; ISS:UniProtKB.
DR   GO; GO:1901315; P:negative regulation of histone H2A K63-linked ubiquitination; ISS:UniProtKB.
DR   GO; GO:0071108; P:protein K48-linked deubiquitination; ISS:UniProtKB.
DR   Gene3D; 1.20.1300.20; -; 1.
DR   Gene3D; 3.30.200.60; -; 1.
DR   InterPro; IPR003323; OTU_dom.
DR   InterPro; IPR030298; OTUB1.
DR   InterPro; IPR016615; Otubain.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR019400; Peptidase_C65_otubain.
DR   InterPro; IPR042468; Peptidase_C65_otubain_sub1.
DR   InterPro; IPR042467; Peptidase_C65_otubain_sub2.
DR   PANTHER; PTHR12931; PTHR12931; 1.
DR   PANTHER; PTHR12931:SF19; PTHR12931:SF19; 1.
DR   Pfam; PF10275; Peptidase_C65; 1.
DR   PIRSF; PIRSF013503; Ubiquitin_thioesterase_Otubain; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS50802; OTU; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Adaptive immunity; Cytoplasm; DNA damage; DNA repair;
KW   Hydrolase; Immunity; Phosphoprotein; Protease; Reference proteome;
KW   Thiol protease; Ubl conjugation pathway.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   CHAIN           2..271
FT                   /note="Ubiquitin thioesterase OTUB1"
FT                   /id="PRO_0000349125"
FT   DOMAIN          80..271
FT                   /note="OTU"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00139"
FT   REGION          130..138
FT                   /note="Ubiquitin-conjugating enzyme E2 binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   REGION          169..177
FT                   /note="Ubiquitin-conjugating enzyme E2 binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   REGION          189..195
FT                   /note="Free ubiquitin binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   REGION          206..213
FT                   /note="Ubiquitin-conjugating enzyme E2 binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   REGION          214..221
FT                   /note="Free ubiquitin binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   REGION          245..251
FT                   /note="Free ubiquitin binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   ACT_SITE        88
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   ACT_SITE        91
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   ACT_SITE        265
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   SITE            23
FT                   /note="Required for proximal ubiquitin-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            221
FT                   /note="Interacts with free ubiquitin"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   SITE            235
FT                   /note="Interacts with free ubiquitin"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   SITE            237
FT                   /note="Interacts with free ubiquitin"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   SITE            261
FT                   /note="Interacts with free ubiquitin"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   SITE            266
FT                   /note="Interacts with free ubiquitin"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
FT   MOD_RES         16
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96FW1"
SQ   SEQUENCE   271 AA;  31270 MW;  32F78EE1DC5FD679 CRC64;
     MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE
     YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK
     SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS
     GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE
     GGTTNPHVFP EGSEPKVYLL YRPGHYDILY K
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024