OTULL_MOUSE
ID OTULL_MOUSE Reviewed; 353 AA.
AC Q3TVP5; Q3U8Q4; Q80WP7;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Inactive ubiquitin thioesterase OTULINL;
GN Name=Otulinl {ECO:0000312|MGI:MGI:2687281};
GN Synonyms=Fam105a {ECO:0000312|MGI:MGI:2687281};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Lacks deubiquitinase activity.
CC {ECO:0000250|UniProtKB:Q9NUU6}.
CC -!- SUBUNIT: Does not bind ubiquitin or ubiquitin-like proteins.
CC {ECO:0000250|UniProtKB:Q9NUU6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9NUU6}.
CC Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9NUU6};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:Q9NUU6}. Nucleus
CC envelope {ECO:0000250|UniProtKB:Q9NUU6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3TVP5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3TVP5-2; Sequence=VSP_022738;
CC -!- DOMAIN: The N-terminal region that precedes the OTU domain mediates
CC interaction with cellular membranes. {ECO:0000250|UniProtKB:Q9NUU6}.
CC -!- SIMILARITY: Belongs to the peptidase C65 family. Otulin subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: Although highly similar to the deubiquitinase OTULIN, lacks
CC the conserved active site Cys at position 136 which is replaced by an
CC Asp residue, and does not show deubiquitinase activity.
CC {ECO:0000250|UniProtKB:Q9NUU6}.
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DR EMBL; AK150760; BAE29828.1; -; mRNA.
DR EMBL; AK152120; BAE30963.1; -; mRNA.
DR EMBL; AK152590; BAE31339.1; -; mRNA.
DR EMBL; AK160030; BAE35573.1; -; mRNA.
DR EMBL; BC052328; AAH52328.1; -; mRNA.
DR CCDS; CCDS27403.1; -. [Q3TVP5-2]
DR CCDS; CCDS88743.1; -. [Q3TVP5-1]
DR RefSeq; NP_001229352.1; NM_001242423.1. [Q3TVP5-1]
DR RefSeq; NP_938043.1; NM_198301.2. [Q3TVP5-2]
DR AlphaFoldDB; Q3TVP5; -.
DR SMR; Q3TVP5; -.
DR iPTMnet; Q3TVP5; -.
DR PhosphoSitePlus; Q3TVP5; -.
DR SwissPalm; Q3TVP5; -.
DR EPD; Q3TVP5; -.
DR MaxQB; Q3TVP5; -.
DR PeptideAtlas; Q3TVP5; -.
DR PRIDE; Q3TVP5; -.
DR ProteomicsDB; 275489; -. [Q3TVP5-1]
DR ProteomicsDB; 275490; -. [Q3TVP5-2]
DR Antibodypedia; 22546; 131 antibodies from 21 providers.
DR Ensembl; ENSMUST00000100739; ENSMUSP00000098305; ENSMUSG00000056069. [Q3TVP5-2]
DR Ensembl; ENSMUST00000226145; ENSMUSP00000154664; ENSMUSG00000056069. [Q3TVP5-1]
DR GeneID; 223433; -.
DR KEGG; mmu:223433; -.
DR UCSC; uc007vjs.2; mouse. [Q3TVP5-2]
DR UCSC; uc007vjt.2; mouse. [Q3TVP5-1]
DR CTD; 54491; -.
DR MGI; MGI:2687281; Otulinl.
DR VEuPathDB; HostDB:ENSMUSG00000056069; -.
DR GeneTree; ENSGT00390000009802; -.
DR HOGENOM; CLU_051856_0_0_1; -.
DR InParanoid; Q3TVP5; -.
DR OMA; GKLRKCI; -.
DR OrthoDB; 1416236at2759; -.
DR PhylomeDB; Q3TVP5; -.
DR TreeFam; TF328709; -.
DR BioGRID-ORCS; 223433; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Otulinl; mouse.
DR PRO; PR:Q3TVP5; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q3TVP5; protein.
DR Bgee; ENSMUSG00000056069; Expressed in skin of snout and 210 other tissues.
DR ExpressionAtlas; Q3TVP5; baseline and differential.
DR Genevisible; Q3TVP5; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0042406; C:extrinsic component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR InterPro; IPR023235; FAM105.
DR InterPro; IPR023236; OTULINL.
DR PANTHER; PTHR33662; PTHR33662; 1.
DR Pfam; PF16218; Peptidase_C101; 1.
DR PRINTS; PR02055; PROTEINF105.
DR PRINTS; PR02056; PROTEINF105A.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Endoplasmic reticulum; Membrane; Nucleus;
KW Reference proteome.
FT CHAIN 1..353
FT /note="Inactive ubiquitin thioesterase OTULINL"
FT /id="PRO_0000274405"
FT DOMAIN 125..353
FT /note="OTU"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00139"
FT REGION 1..80
FT /note="Required for membrane binding"
FT /evidence="ECO:0000250|UniProtKB:Q9NUU6"
FT VAR_SEQ 114..163
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022738"
FT CONFLICT 173
FT /note="G -> S (in Ref. 1; BAE29828/BAE30963)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 353 AA; 41576 MW; DCCB7047E1CFDF30 CRC64;
MEAPRSAPRE RERARTTSGS DQVHSWILVT SQVLSAAWRI ARAFVMTTLS PLSATFSYFR
SLYLYLGHQL KWWIGYLQRK FKRNLSVEAE VDLLSYCARE WKGEAPRARL MRKAYEELFW
RHHIKCVRAV KRDNYDALRS VLFQIFSQGL SFPSWMKEKD IVKLPEKLLF SQGCNWIQQY
SFGPEKYTGS NVFGKLRKCV ELLKLQWTEF SGMRDHHKRG SMCNSLFSDA ILECKLYEAL
KFLMLYQVTE AYEQMKTNKV IPSLFRLLFS RESSPDPLSF MMNHLNSIGD TCGLDQIDMF
ILGYSLQVKI KVFRLFKFNS RDFAVCYPEE PLREWPEISL LTENGHHYHI PVF
