OTX2A_XENLA
ID OTX2A_XENLA Reviewed; 288 AA.
AC Q91813; Q6DDZ3; Q91811; Q91812;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Homeobox protein OTX2-A;
DE Short=xOTX2-A;
DE AltName: Full=Orthodenticle 2-A;
DE AltName: Full=Orthodenticle-A-like protein A;
GN Name=otx2-a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND MUTAGENESIS OF LYS-87.
RC TISSUE=Embryo;
RX PubMed=7720578; DOI=10.1242/dev.121.3.707;
RA Pannese M., Polo C., Andreazzoli M., Vignali R., Kablar B., Barsacchi G.,
RA Boncinelli E.;
RT "The Xenopus homologue of Otx2 is a maternal homeobox gene that demarcates
RT and specifies anterior body regions.";
RL Development 121:707-720(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-102.
RC TISSUE=Head;
RX PubMed=8235591; DOI=10.1126/science.8235591;
RA Lamb T.M., Knecht A.K., Smith W.C., Stachel S.E., Economides A.N.,
RA Stahl N., Yancopolous G.D., Harland R.M.;
RT "Neural induction by the secreted polypeptide noggin.";
RL Science 262:713-718(1993).
RN [4]
RP FUNCTION.
RX PubMed=10926781; DOI=10.1006/dbio.2000.9778;
RA Mochizuki T., Karavanov A.A., Curtiss P.E., Ault K.T., Sugimoto N.,
RA Watabe T., Shiokawa K., Jamrich M., Cho K.W.Y., Dawid I.B., Taira M.;
RT "Xlim-1 and LIM domain binding protein 1 cooperate with various
RT transcription factors in the regulation of the goosecoid promoter.";
RL Dev. Biol. 224:470-485(2000).
RN [5]
RP FUNCTION.
RX PubMed=12710967; DOI=10.1016/s0012-1606(03)00034-4;
RA Yamamoto S., Hikasa H., Ono H., Taira M.;
RT "Molecular link in the sequential induction of the Spemann organizer:
RT direct activation of the cerberus gene by Xlim-1, Xotx2, Mix.1, and
RT Siamois, immediately downstream from Nodal and Wnt signaling.";
RL Dev. Biol. 257:190-204(2003).
CC -!- FUNCTION: May play a central role in the initial events of axis
CC formation and in particular in specifying anterior head regions and
CC their spatial relationship with trunk structures. Activates the head
CC organizer gene cer1 by acting synergistically with siamois and mix-
CC A/mix.1 through the 5'-TAATCT-3' element of the cer1 promoter. Also
CC binds as a complex with lhx1/lim1 and ldb1 to the gsc promoter to
CC stimulate expression. {ECO:0000269|PubMed:10926781,
CC ECO:0000269|PubMed:12710967, ECO:0000269|PubMed:7720578}.
CC -!- INTERACTION:
CC Q91813; O42569: sox2; NbExp=5; IntAct=EBI-15695802, EBI-15695825;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Shows a localized expression in the dorsal region
CC of the marginal zone at stage 9.5. At stage 10.5, expressed in dorsal
CC bottle cells of the dorsal deep zone fated to give rise to prechordal
CC mesendoderm. Also begins to be expressed in presumptive anterior
CC neuroectoderm where expression persists in subsequent stages. At stage
CC 14, expression is confined to mesendoderm and ectoderm cells of
CC anterior dorsal regions. {ECO:0000269|PubMed:7720578}.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels throughout early
CC development from unfertilized egg to late blastula when its expression
CC level significantly increases. {ECO:0000269|PubMed:7720578}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; Z46972; CAA87093.1; -; mRNA.
DR EMBL; BC077357; AAH77357.1; -; mRNA.
DR EMBL; L26509; AAA85388.1; -; mRNA.
DR PIR; I51620; I51620.
DR RefSeq; NP_001084160.1; NM_001090691.1.
DR RefSeq; XP_018087427.1; XM_018231938.1.
DR AlphaFoldDB; Q91813; -.
DR SMR; Q91813; -.
DR IntAct; Q91813; 1.
DR DNASU; 399342; -.
DR GeneID; 399342; -.
DR KEGG; xla:399342; -.
DR CTD; 399342; -.
DR Xenbase; XB-GENE-6254039; otx2.S.
DR OMA; PHPKGPW; -.
DR OrthoDB; 1481439at2759; -.
DR Proteomes; UP000186698; Chromosome 8S.
DR Bgee; 399342; Expressed in gastrula and 9 other tissues.
DR GO; GO:0005634; C:nucleus; IC:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR003022; Otx2_TF.
DR InterPro; IPR003025; Otx_TF.
DR InterPro; IPR013851; Otx_TF_C.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03529; TF_Otx; 1.
DR PRINTS; PR01257; OTX2HOMEOBOX.
DR PRINTS; PR01255; OTXHOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..288
FT /note="Homeobox protein OTX2-A"
FT /id="PRO_0000049217"
FT DNA_BIND 38..97
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 93..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..140
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 87
FT /note="Determines DNA-binding specificity"
FT MUTAGEN 87
FT /note="K->E: Fails to produce a phenotype when injected."
FT /evidence="ECO:0000269|PubMed:7720578"
FT MUTAGEN 87
FT /note="K->Q: Produces a proportion of abnormal phenotypes
FT when injected, but these are substantially different from
FT those for intact otx2-A injections."
FT /evidence="ECO:0000269|PubMed:7720578"
FT CONFLICT 102
FT /note="N -> Q (in Ref. 3; AAA85388)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 288 AA; 31582 MW; AEB2F2948D51F6D4 CRC64;
MMSYLKQPPY AVNGLSLTAS GMDLLHQSVG YPATPRKQRR ERTTFTRAQL DILEALFAKT
RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQQQ QNGGQNKVRP SKKKTSPVRE
VSSESGTSGQ FSPPSSTSVP VISSSTAPVS IWSPASVSPL SDPLSTSSSC MQRSYPMTYT
QASGYSQGYA GSTSYFGGMD CGSYLSPMHH QLSGPGATLS PMGTNAVTSH LNQSPVALSS
QAYGASSLGF NSTDCLDYKD QTASWKLNFN ADCLDYKDQT SSWKFQVL
