OTX2_MOUSE
ID OTX2_MOUSE Reviewed; 289 AA.
AC P80206;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Homeobox protein OTX2;
DE AltName: Full=Orthodenticle homolog 2;
GN Name=Otx2; Synonyms=Otx-2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=8101484; DOI=10.1002/j.1460-2075.1993.tb05935.x;
RA Simeone A., Acampora D., Mallamaci A., Stornaiuolo A., D'Apice M.R.,
RA Nigro V., Boncinelli E.;
RT "A vertebrate gene related to orthodenticle contains a homeodomain of the
RT bicoid class and demarcates anterior neuroectoderm in the gastrulating
RT mouse embryo.";
RL EMBO J. 12:2735-2747(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 31-98.
RC TISSUE=Embryo;
RX PubMed=1353865; DOI=10.1038/358687a0;
RA Simeone A., Acampora D., Gulisano M., Stornaiuolo A., Boncinelli E.;
RT "Nested expression domains of four homeobox genes in developing rostral
RT brain.";
RL Nature 358:687-690(1992).
RN [3]
RP MUTAGENESIS OF ASN-225.
RX PubMed=18728160; DOI=10.1210/jc.2008-1189;
RA Diaczok D., Romero C., Zunich J., Marshall I., Radovick S.;
RT "A novel dominant negative mutation of OTX2 associated with combined
RT pituitary hormone deficiency.";
RL J. Clin. Endocrinol. Metab. 93:4351-4359(2008).
RN [4]
RP STRUCTURE BY NMR OF 39-105.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the homeobox domain of homeobox protein OTX2.";
RL Submitted (OCT-2006) to the PDB data bank.
CC -!- FUNCTION: Transcription factor probably involved in the development of
CC the brain and the sense organs. Can bind to the bicoid/BCD target
CC sequence (BTS): 5'-TCTAATCCC-3'. {ECO:0000250|UniProtKB:P32243}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P32243}.
CC -!- TISSUE SPECIFICITY: Brain: restricted regions of the developing rostral
CC brain including the presumptive cerebral cortex and olfactory bulbs;
CC expressed in the developing olfactory, auricolar and ocular systems,
CC including the covering of the optic nerve.
CC -!- DEVELOPMENTAL STAGE: Embryo.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; X68884; CAA48755.1; -; mRNA.
DR CCDS; CCDS49473.1; -.
DR PIR; S35346; S35346.
DR PDB; 2DMS; NMR; -; A=39-105.
DR PDBsum; 2DMS; -.
DR AlphaFoldDB; P80206; -.
DR BMRB; P80206; -.
DR SMR; P80206; -.
DR CORUM; P80206; -.
DR MINT; P80206; -.
DR STRING; 10090.ENSMUSP00000112532; -.
DR iPTMnet; P80206; -.
DR PhosphoSitePlus; P80206; -.
DR PaxDb; P80206; -.
DR PRIDE; P80206; -.
DR ProteomicsDB; 294137; -.
DR MGI; MGI:97451; Otx2.
DR eggNOG; KOG2251; Eukaryota.
DR InParanoid; P80206; -.
DR PhylomeDB; P80206; -.
DR ChiTaRS; Otx2; mouse.
DR EvolutionaryTrace; P80206; -.
DR PRO; PR:P80206; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P80206; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0030426; C:growth cone; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; TAS:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0048856; P:anatomical structure development; IGI:MGI.
DR GO; GO:0009887; P:animal organ morphogenesis; IMP:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR GO; GO:0007411; P:axon guidance; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; TAS:MGI.
DR GO; GO:0045165; P:cell fate commitment; IMP:MGI.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEP:MGI.
DR GO; GO:0007417; P:central nervous system development; IMP:MGI.
DR GO; GO:0048852; P:diencephalon morphogenesis; IGI:MGI.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:MGI.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI.
DR GO; GO:0007492; P:endoderm development; IGI:MGI.
DR GO; GO:0042706; P:eye photoreceptor cell fate commitment; IMP:MGI.
DR GO; GO:0030900; P:forebrain development; IMP:MGI.
DR GO; GO:0042472; P:inner ear morphogenesis; IGI:MGI.
DR GO; GO:0048382; P:mesendoderm development; IMP:MGI.
DR GO; GO:0022037; P:metencephalon development; IGI:MGI.
DR GO; GO:0030901; P:midbrain development; IMP:MGI.
DR GO; GO:0060563; P:neuroepithelial cell differentiation; IMP:MGI.
DR GO; GO:0048663; P:neuron fate commitment; IMP:MGI.
DR GO; GO:0048664; P:neuron fate determination; IMP:MGI.
DR GO; GO:0048665; P:neuron fate specification; IMP:MGI.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IMP:MGI.
DR GO; GO:0040019; P:positive regulation of embryonic development; IMP:UniProtKB.
DR GO; GO:2000543; P:positive regulation of gastrulation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0090009; P:primitive streak formation; IMP:UniProtKB.
DR GO; GO:0065003; P:protein-containing complex assembly; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0032525; P:somite rostral/caudal axis specification; IMP:MGI.
DR GO; GO:0021978; P:telencephalon regionalization; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR003022; Otx2_TF.
DR InterPro; IPR003025; Otx_TF.
DR InterPro; IPR013851; Otx_TF_C.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03529; TF_Otx; 1.
DR PRINTS; PR01257; OTX2HOMEOBOX.
DR PRINTS; PR01255; OTXHOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome.
FT CHAIN 1..289
FT /note="Homeobox protein OTX2"
FT /id="PRO_0000049211"
FT DNA_BIND 38..97
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 93..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..109
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 225
FT /note="N->S: Has a dominant negative effect on the proximal
FT promoter region of HESX1 gene; represses reporter
FT expression."
FT /evidence="ECO:0000269|PubMed:18728160"
FT HELIX 47..59
FT /evidence="ECO:0007829|PDB:2DMS"
FT HELIX 65..74
FT /evidence="ECO:0007829|PDB:2DMS"
FT HELIX 79..90
FT /evidence="ECO:0007829|PDB:2DMS"
FT HELIX 92..95
FT /evidence="ECO:0007829|PDB:2DMS"
SQ SEQUENCE 289 AA; 31622 MW; B4EA3A5BF83E2D99 CRC64;
MMSYLKQPPY AVNGLSLTTS GMDLLHPSVG YPATPRKQRR ERTTFTRAQL DVLEALFAKT
RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQQQ QNGGQNKVRP AKKKSSPARE
VSSESGTSGQ FTPPSSTSVP TIASSSAPVS IWSPASISPL SDPLSTSSSC MQRSYPMTYT
QASGYSQGYA GSTSYFGGMD CGSYLTPMHH QLPGPGATLS PMGTNAVTSH LNQSPASLST
QGYGASSLGF NSTTDCLDYK DQTASWKLNF NADCLDYKDQ TSSWKFQVL
