OUSV_DICD3
ID OUSV_DICD3 Reviewed; 400 AA.
AC E0SCY1; Q71JD0;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Glycine betaine/choline transport system ATP-binding protein OusV {ECO:0000305};
GN Name=ousV {ECO:0000303|PubMed:16000740};
GN Synonyms=ousBV {ECO:0000312|EMBL:AAQ06630.1};
GN OrderedLocusNames=Dda3937_00901 {ECO:0000312|EMBL:ADM99772.1};
OS Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Dickeya.
OX NCBI_TaxID=198628;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=3937;
RX PubMed=16000740; DOI=10.1128/aem.71.7.3389-3398.2005;
RA Choquet G., Jehan N., Pissavin C., Blanco C., Jebbar M.;
RT "OusB, a broad-specificity ABC-type transporter from Erwinia chrysanthemi,
RT mediates uptake of glycine betaine and choline with a high affinity.";
RL Appl. Environ. Microbiol. 71:3389-3398(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3937;
RX PubMed=21217001; DOI=10.1128/jb.01513-10;
RA Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA Blattner F.R., Keen N.T., Perna N.T.;
RT "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL J. Bacteriol. 193:2076-2077(2011).
CC -!- FUNCTION: Part of the OusB ABC transporter complex involved in glycine
CC betaine and choline uptake. May also transport several other
CC osmoprotectants such as ectoine, DMSA, DMSP, proline and carnitine.
CC Probably responsible for energy coupling to the transport system.
CC {ECO:0000269|PubMed:16000740}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (OusV),
CC two transmembrane proteins (OusW) and a solute-binding protein (OusX).
CC {ECO:0000305|PubMed:16000740}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P14175}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P14175}.
CC -!- INDUCTION: Induced by increased medium osmolality. Repressed by glycine
CC betaine. {ECO:0000269|PubMed:16000740}.
CC -!- DISRUPTION PHENOTYPE: Uptake of glycine betaine and choline is
CC completely abolished in the ousA-ousB double mutant.
CC {ECO:0000269|PubMed:16000740}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR EMBL; AF494101; AAQ06630.1; -; Genomic_DNA.
DR EMBL; CP002038; ADM99772.1; -; Genomic_DNA.
DR RefSeq; WP_013319197.1; NC_014500.1.
DR AlphaFoldDB; E0SCY1; -.
DR SMR; E0SCY1; -.
DR STRING; 198628.Dda3937_00901; -.
DR EnsemblBacteria; ADM99772; ADM99772; Dda3937_00901.
DR GeneID; 9735043; -.
DR KEGG; ddd:Dda3937_00901; -.
DR PATRIC; fig|198628.6.peg.3519; -.
DR eggNOG; COG4175; Bacteria.
DR HOGENOM; CLU_000604_2_2_6; -.
DR OMA; GQIFVVM; -.
DR OrthoDB; 1713578at2; -.
DR BioCyc; DDAD198628:DDA3937_RS16720-MON; -.
DR Proteomes; UP000006859; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0031460; P:glycine betaine transport; IEA:InterPro.
DR Gene3D; 3.10.580.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR005892; Gly-betaine_transp_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF00571; CBS; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR TIGRFAMs; TIGR01186; proV; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51371; CBS; 2.
PE 1: Evidence at protein level;
KW Amino-acid transport; ATP-binding; CBS domain; Cell inner membrane;
KW Cell membrane; Membrane; Nucleotide-binding; Reference proteome; Repeat;
KW Transport.
FT CHAIN 1..400
FT /note="Glycine betaine/choline transport system ATP-binding
FT protein OusV"
FT /id="PRO_0000441735"
FT DOMAIN 29..265
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 280..339
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 343..400
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT BINDING 61..68
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 400 AA; 44338 MW; 39AAD164408DC872 CRC64;
MAIKLEVTHL YKIFGEHPER AFRLLEQGLS KDQIFEKTGL TVGVKDASLA IEEGEIFVIM
GLSGSGKSTL VRLLNRLIEP TRGQVLIDGE DISRLPDGAL RAVRRKQISM VFQSFALMPH
LNILDNTAFG MDLAGVPRAE REQKALNALQ QVGLETYAHA YPDELSGGMR QRVGLARALA
NDPDILLMDE AFSALDPLIR TEMQDELIKL QARRQRTIVF ISHDLDEAMR IGDRIAIMHS
GEVIQVGTPD EILNNPANDY VRTFFRGVDI SHVFTAKDIA RRRPVAVIRK TPGVGPRSAL
RILQEEDREY GYVLERGRKF IGVVSIDSLK QALRGQQPLE QALLPAPAPV PASMSLNELI
SQVAQAPCAV PVVDENHEYL GIISKGMLLQ ALDKESTLND
