ID   OUSX_DICD3              Reviewed;         332 AA.
AC   E0SCY3; Q71JC8;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Glycine betaine-binding periplasmic protein OusX {ECO:0000305};
DE   Flags: Precursor;
GN   Name=ousX {ECO:0000303|PubMed:16000740};
GN   Synonyms=ousBX {ECO:0000312|EMBL:AAQ06632.1};
GN   OrderedLocusNames=Dda3937_00899 {ECO:0000312|EMBL:ADM99774.1};
OS   Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Dickeya.
OX   NCBI_TaxID=198628;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION,
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=3937;
RX   PubMed=16000740; DOI=10.1128/aem.71.7.3389-3398.2005;
RA   Choquet G., Jehan N., Pissavin C., Blanco C., Jebbar M.;
RT   "OusB, a broad-specificity ABC-type transporter from Erwinia chrysanthemi,
RT   mediates uptake of glycine betaine and choline with a high affinity.";
RL   Appl. Environ. Microbiol. 71:3389-3398(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3937;
RX   PubMed=21217001; DOI=10.1128/jb.01513-10;
RA   Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA   Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA   Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA   Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA   Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA   Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA   Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA   Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA   Blattner F.R., Keen N.T., Perna N.T.;
RT   "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL   J. Bacteriol. 193:2076-2077(2011).
CC   -!- FUNCTION: Part of the OusB ABC transporter complex involved in glycine
CC       betaine and choline uptake. Binds glycine betaine.
CC       {ECO:0000269|PubMed:16000740}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (OusV),
CC       two transmembrane proteins (OusW) and a solute-binding protein (OusX).
CC       {ECO:0000305|PubMed:16000740}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:16000740}.
CC   -!- INDUCTION: Induced by increased medium osmolality. Repressed by glycine
CC       betaine. {ECO:0000269|PubMed:16000740}.
CC   -!- DISRUPTION PHENOTYPE: Uptake of glycine betaine and choline is
CC       completely abolished in the ousA-ousB double mutant.
CC       {ECO:0000269|PubMed:16000740}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF494101; AAQ06632.1; -; Genomic_DNA.
DR   EMBL; CP002038; ADM99774.1; -; Genomic_DNA.
DR   RefSeq; WP_013319199.1; NC_014500.1.
DR   AlphaFoldDB; E0SCY3; -.
DR   SMR; E0SCY3; -.
DR   STRING; 198628.Dda3937_00899; -.
DR   EnsemblBacteria; ADM99774; ADM99774; Dda3937_00899.
DR   GeneID; 9735045; -.
DR   KEGG; ddd:Dda3937_00899; -.
DR   PATRIC; fig|198628.6.peg.3521; -.
DR   eggNOG; COG2113; Bacteria.
DR   HOGENOM; CLU_070055_0_0_6; -.
DR   OMA; EHNQGNY; -.
DR   OrthoDB; 485527at2; -.
DR   BioCyc; DDAD198628:DDA3937_RS16730-MON; -.
DR   Proteomes; UP000006859; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007210; ABC_Gly_betaine_transp_sub-bd.
DR   Pfam; PF04069; OpuAC; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Periplasm; Reference proteome; Signal; Transport.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..332
FT                   /note="Glycine betaine-binding periplasmic protein OusX"
FT                   /id="PRO_5003140232"
SQ   SEQUENCE   332 AA;  36357 MW;  4650B84FA296AF7C CRC64;
     MRNISMATLA LTTVLSTGLF AADDLPGKGI TVKPVQSTIS EETFQTLLVS KALEKLGYTV
     DKPSEVDYNV GYTSIANGDA TFTAVNWQPL HDDMYQAAGG DAKFYRQGVY VSGAAQGYLI
     DKKTAERYHI TRLDQLKDPK LAKLFDTNGD GKADLTGCNP GWGCDSVINH QIQAYGLGDT
     VNHNQGNYAA LIADTIARYK QGKSVIFFTW TPYWVSDVLV PGRDVVWLQV PFSSLPGKQK
     GTDTKLPNGA NYGFPVNNMR IVANKDWAEK NPAAAKLFAI MKLPLADINA QNLRMHQGEA
     SQQDIERHVN GWINAHQAQF DGWLNAARAA AK