位置:首页 > 蛋白库 > ASD2_CAEEL
ASD2_CAEEL
ID   ASD2_CAEEL              Reviewed;         445 AA.
AC   G5EFF1; G5EFG9; O02065;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=RNA-binding protein asd-2 {ECO:0000305};
DE   AltName: Full=Alternative splicing defective protein 2 {ECO:0000303|PubMed:18230701};
GN   Name=asd-2 {ECO:0000303|PubMed:18230701, ECO:0000312|WormBase:T21G5.5c};
GN   Synonyms=let-529 {ECO:0000312|WormBase:T21G5.5c},
GN   star-2 {ECO:0000303|PubMed:20573244, ECO:0000312|WormBase:T21G5.5c},
GN   tag-44 {ECO:0000312|WormBase:T21G5.5c};
GN   ORFNames=T21G5.5 {ECO:0000312|WormBase:T21G5.5c};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|EMBL:ABU96120.1}
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C), FUNCTION, TISSUE
RP   SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP   GLU-244.
RC   STRAIN=Bristol N2 {ECO:0000312|EMBL:ABU96120.1};
RX   PubMed=18230701; DOI=10.1101/gad.1620608;
RA   Ohno G., Hagiwara M., Kuroyanagi H.;
RT   "STAR family RNA-binding protein ASD-2 regulates developmental switching of
RT   mutually exclusive alternative splicing in vivo.";
RL   Genes Dev. 22:360-374(2008).
RN   [2] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [3] {ECO:0000305}
RP   FUNCTION, AND DOMAIN.
RX   PubMed=20573244; DOI=10.1186/1471-2199-11-48;
RA   Carmel A.B., Wu J., Lehmann-Blount K.A., Williamson J.R.;
RT   "High-affinity consensus binding of target RNAs by the STAR/GSG proteins
RT   GLD-1, STAR-2 and Quaking.";
RL   BMC Mol. Biol. 11:48-48(2010).
RN   [4] {ECO:0000305}
RP   FUNCTION, INTERACTION WITH SUP-12, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=23071450; DOI=10.1371/journal.pgen.1002991;
RA   Ohno G., Ono K., Togo M., Watanabe Y., Ono S., Hagiwara M., Kuroyanagi H.;
RT   "Muscle-specific splicing factors ASD-2 and SUP-12 cooperatively switch
RT   alternative pre-mRNA processing patterns of the ADF/cofilin gene in
RT   Caenorhabditis elegans.";
RL   PLoS Genet. 8:E1002991-E1002991(2012).
CC   -!- FUNCTION: RNA-binding protein that binds to the 5'-NACUAAY-N(1,20)-
CC       UAAY-3' consensus sequence in pre-mRNA introns to promote alternative
CC       splicing (PubMed:18230701, PubMed:20573244, PubMed:23071450). Required
CC       for mutually exclusive alternative splicing where it modulates the
CC       switch between mutually exclusive exons during pre-mRNA maturation
CC       (PubMed:18230701, PubMed:23071450). Involved in muscle-specific gene
CC       expression regulating the alternative splicing of genes such as let-2
CC       and unc-60 to ensure that their respective isoforms are expressed in
CC       muscle (PubMed:18230701, PubMed:23071450). Promotes the removal of
CC       intron 10 from let-2 pre-mRNA to allow for the exclusive expression of
CC       the muscle-specific let-2 isoform (as opposed to the non-muscle-
CC       specific isoform expressed in embryos) in body wall muscles during late
CC       larval and adult stages of development (PubMed:18230701). Binds
CC       cooperatively with RNA-binding protein sup-12 to intron 1A of the unc-
CC       60 pre-mRNA to promote alternative splicing and expression of the
CC       muscle specific isoform of unc-60 (PubMed:23071450).
CC       {ECO:0000269|PubMed:18230701, ECO:0000269|PubMed:20573244,
CC       ECO:0000269|PubMed:23071450}.
CC   -!- SUBUNIT: Interacts with sup-12; in the presence of RNA, but with weak
CC       affinity in the absence of RNA. {ECO:0000269|PubMed:23071450}.
CC   -!- INTERACTION:
CC       G5EFF1; Q20084: exc-7; NbExp=3; IntAct=EBI-316859, EBI-321225;
CC       G5EFF1; G5ECJ4: mec-8; NbExp=4; IntAct=EBI-316859, EBI-317668;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23071450}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=c {ECO:0000312|WormBase:T21G5.5c}; Synonyms=b
CC       {ECO:0000303|PubMed:18230701, ECO:0000303|PubMed:23071450};
CC         IsoId=G5EFF1-1; Sequence=Displayed;
CC       Name=a {ECO:0000312|WormBase:T21G5.5a};
CC         IsoId=G5EFF1-2; Sequence=VSP_058956, VSP_058957, VSP_058958;
CC       Name=b {ECO:0000312|WormBase:T21G5.5b}; Synonyms=a
CC       {ECO:0000303|PubMed:18230701};
CC         IsoId=G5EFF1-3; Sequence=VSP_058956;
CC   -!- TISSUE SPECIFICITY: Isoform b: Expressed in the hypodermis and
CC       pharyngeal muscles (PubMed:18230701). Isoform c: Expressed in body wall
CC       muscles and phayngeal muscles (PubMed:18230701, PubMed:23071450).
CC       {ECO:0000269|PubMed:18230701, ECO:0000269|PubMed:23071450}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during embryonic development.
CC       {ECO:0000269|PubMed:18230701}.
CC   -!- DOMAIN: The KH domain and the Qua2 region are involved in RNA binding.
CC       {ECO:0000305|PubMed:20573244}.
CC   -!- DISRUPTION PHENOTYPE: Defective alternative splicing of the unc-60 gene
CC       which results in decreased expression of the muscle-specific isoform of
CC       unc-60 (PubMed:23071450). Double knockout with isoform c of unc-60
CC       rescues the motility and actin filament disorganization defects in the
CC       single unc-60 isoform c mutant (PubMed:23071450). RNAi-mediated
CC       knockdown results animals that are viable throughout embryonic and
CC       larval development, and furthermore, results in defective alternative
CC       splicing of the let-2 gene, whereby transcripts containing exon 10 are
CC       not formed in larvae and so the muscle-specific isoform of let-2 is not
CC       expressed in body wall muscles (PubMed:18230701). RNAi-mediated
CC       knockdown results in abnormal ectopic expression of the non-muscle-
CC       specific isoform b of unc-60 in body wall muscles (PubMed:23071450).
CC       {ECO:0000269|PubMed:18230701, ECO:0000269|PubMed:23071450}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EF630625; ABU96119.1; -; mRNA.
DR   EMBL; EF630626; ABU96120.1; -; mRNA.
DR   EMBL; BX284601; CCD67593.1; -; Genomic_DNA.
DR   EMBL; BX284601; CCD67594.1; -; Genomic_DNA.
DR   EMBL; BX284601; CCD67595.1; -; Genomic_DNA.
DR   PIR; T15136; T15136.
DR   RefSeq; NP_001021625.1; NM_001026454.3. [G5EFF1-2]
DR   RefSeq; NP_001021626.1; NM_001026455.3. [G5EFF1-3]
DR   RefSeq; NP_001021627.1; NM_001026456.2. [G5EFF1-1]
DR   AlphaFoldDB; G5EFF1; -.
DR   SMR; G5EFF1; -.
DR   DIP; DIP-26760N; -.
DR   IntAct; G5EFF1; 19.
DR   STRING; 6239.T21G5.5d; -.
DR   EnsemblMetazoa; T21G5.5a.1; T21G5.5a.1; WBGene00006423. [G5EFF1-2]
DR   EnsemblMetazoa; T21G5.5b.1; T21G5.5b.1; WBGene00006423. [G5EFF1-3]
DR   EnsemblMetazoa; T21G5.5b.2; T21G5.5b.2; WBGene00006423. [G5EFF1-3]
DR   EnsemblMetazoa; T21G5.5c.1; T21G5.5c.1; WBGene00006423. [G5EFF1-1]
DR   GeneID; 188703; -.
DR   UCSC; T21G5.5c; c. elegans.
DR   CTD; 188703; -.
DR   WormBase; T21G5.5a; CE32731; WBGene00006423; asd-2. [G5EFF1-2]
DR   WormBase; T21G5.5b; CE13896; WBGene00006423; asd-2. [G5EFF1-3]
DR   WormBase; T21G5.5c; CE37411; WBGene00006423; asd-2. [G5EFF1-1]
DR   eggNOG; KOG1588; Eukaryota.
DR   GeneTree; ENSGT00940000167937; -.
DR   OMA; ISHAGAM; -.
DR   PhylomeDB; G5EFF1; -.
DR   PRO; PR:G5EFF1; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00006423; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR   ExpressionAtlas; G5EFF1; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IDA:WormBase.
DR   GO; GO:0043186; C:P granule; IDA:WormBase.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IPI:WormBase.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0097157; F:pre-mRNA intronic binding; IPI:WormBase.
DR   GO; GO:0003727; F:single-stranded RNA binding; IDA:WormBase.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:WormBase.
DR   GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; IBA:GO_Central.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1370.10; -; 1.
DR   InterPro; IPR045071; BBP-like.
DR   InterPro; IPR004087; KH_dom.
DR   InterPro; IPR004088; KH_dom_type_1.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR032377; STAR_dimer.
DR   PANTHER; PTHR11208; PTHR11208; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF16544; STAR_dimer; 1.
DR   SMART; SM00322; KH; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..445
FT                   /note="RNA-binding protein asd-2"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000440162"
FT   DOMAIN          145..171
FT                   /note="KH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT   REGION          22..63
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          71..128
FT                   /note="Qua1 domain"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   REGION          230..253
FT                   /note="Qua2 domain; involved in RNA binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   COMPBIAS        41..63
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            145
FT                   /note="Involved in RNA binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   SITE            153
FT                   /note="Important for the interaction between KH and Qua2
FT                   domains"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   SITE            168
FT                   /note="Involved in RNA binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   SITE            172
FT                   /note="Involved in RNA binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   SITE            178
FT                   /note="Important for RNA binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   SITE            238
FT                   /note="Involved in RNA binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   SITE            241
FT                   /note="Involved in RNA binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   SITE            245
FT                   /note="Important for the interaction between KH and Qua2
FT                   domains"
FT                   /evidence="ECO:0000250|UniProtKB:Q17339"
FT   VAR_SEQ         1..54
FT                   /note="MDCDNGVVSEISDDKELLNLETVIPPPPNDSGHEFIGPSSGPPQVTITPSGV
FT                   QS -> MTHASTDLLTPN (in isoform a and isoform b)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_058956"
FT   VAR_SEQ         370
FT                   /note="S -> R (in isoform a)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_058957"
FT   VAR_SEQ         371..445
FT                   /note="Missing (in isoform a)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_058958"
FT   MUTAGEN         244
FT                   /note="E->K: In yb1423; defective alternative splicing of
FT                   the let-2 gene whereby intron 10 within the let-2 pre-mRNA
FT                   is not removed."
FT                   /evidence="ECO:0000269|PubMed:18230701"
SQ   SEQUENCE   445 AA;  47789 MW;  BBF0A250ED49EC52 CRC64;
     MDCDNGVVSE ISDDKELLNL ETVIPPPPND SGHEFIGPSS GPPQVTITPS GVQSGSANGV
     STSQQQQYSA EYLSQLLKDK KQLAAFPNVF HHLERLADEE INKVRVVLFQ CEFSKESAPL
     PDAEGDSTVH TEKVFVPAKE HPDYNFVGRI LGPRGMTAKQ LEQETGCKIM VRGRGSMRDK
     KKEELNRGKP NWEHLSEELH VLIQCEDTEN RAKVKLMRAV EEVRKLLVPA PEGEDDLKRK
     QLMELAIING TYRSGTDQSA LAAAQLAAVK HQQQPFAAAL QAAALQRGVL PMMANGLSRS
     PTMAVCGAPI VMSPSGRASS AGATATSQAA LIMQQQSQLH AANAGNAALQ QQAALLQQQQ
     AAEYQQLLLS QAGLYDFSAM QQQYAAVGQN AAVAAAQAQA QAQYGALAAA AAANSAGNQQ
     YADYAGVDLT SQQSAHGGYY VRRWA
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024