OX1R_RAT
ID OX1R_RAT Reviewed; 416 AA.
AC P56718;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Orexin/Hypocretin receptor type 1 {ECO:0000305|PubMed:11340621};
DE AltName: Full=Hypocretin receptor type 1 {ECO:0000312|RGD:2787};
DE AltName: Full=Orexin receptor type 1 {ECO:0000305|PubMed:9491897};
DE Short=Ox-1-R;
DE Short=Ox1-R;
DE Short=Ox1R;
GN Name=Hcrtr1 {ECO:0000312|RGD:2787};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION BY FASTING.
RC TISSUE=Brain;
RX PubMed=9491897; DOI=10.1016/s0092-8674(00)80949-6;
RA Sakurai T., Amemiya A., Ishii M., Matsuzaki I., Chemelli R.M., Tanaka H.,
RA Williams S.C., Richardson J.A., Kozlowski G.P., Wilson S., Arch J.R.S.,
RA Buckingham R.E., Haynes A.C., Carr S.A., Annan R.S., McNulty D.E.,
RA Liu W.-S., Terrett J.A., Elshourbagy N.A., Bergsma D.J., Yanagisawa M.;
RT "Orexins and orexin receptors: a family of hypothalamic neuropeptides and G
RT protein-coupled receptors that regulate feeding behavior.";
RL Cell 92:573-585(1998).
RN [2]
RP REVIEW.
RX PubMed=11340621; DOI=10.1002/bies.1058;
RA Hungs M., Mignot E.;
RT "Hypocretin/orexin, sleep and narcolepsy.";
RL Bioessays 23:397-408(2001).
RN [3]
RP REVIEW.
RX PubMed=11283317; DOI=10.1146/annurev.neuro.24.1.429;
RA Willie J.T., Chemelli R.M., Sinton C.M., Yanagisawa M.;
RT "To eat or to sleep? Orexin in the regulation of feeding and wakefulness.";
RL Annu. Rev. Neurosci. 24:429-458(2001).
CC -!- FUNCTION: Moderately selective excitatory receptor for orexin-A and,
CC with a lower affinity, for orexin-B neuropeptide. Triggers an increase
CC in cytoplasmic Ca(2+) levels in response to orexin-A binding.
CC {ECO:0000250|UniProtKB:O43613}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O43613};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:O43613}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the brain in the prefrontal
CC cortex, hippocampus, paraventricular thalamus, ventromedial
CC hypothalamus, arcuate nucleus, dorsal raphe nucleus, and locus
CC coeruleus. Not detected in the spleen, lung, liver, skeletal muscle,
CC kidney and testis. Orexin receptor mRNA expression has also been
CC reported in the adrenal gland, enteric nervous system, and pancreas.
CC {ECO:0000269|PubMed:9491897}.
CC -!- INDUCTION: By nutritional state, up-regulated by fasting.
CC {ECO:0000269|PubMed:9491897}.
CC -!- DOMAIN: The N-terminal region is required for orexin signaling.
CC {ECO:0000250|UniProtKB:O43613}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF041244; AAC40041.1; -; mRNA.
DR RefSeq; NP_037196.1; NM_013064.1.
DR AlphaFoldDB; P56718; -.
DR SMR; P56718; -.
DR STRING; 10116.ENSRNOP00000018674; -.
DR BindingDB; P56718; -.
DR ChEMBL; CHEMBL1075232; -.
DR GuidetoPHARMACOLOGY; 321; -.
DR GlyGen; P56718; 1 site.
DR PhosphoSitePlus; P56718; -.
DR PaxDb; P56718; -.
DR DNASU; 25593; -.
DR Ensembl; ENSRNOT00000018674; ENSRNOP00000018674; ENSRNOG00000013838.
DR GeneID; 25593; -.
DR KEGG; rno:25593; -.
DR CTD; 3061; -.
DR RGD; 2787; Hcrtr1.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01020000230390; -.
DR HOGENOM; CLU_009579_6_3_1; -.
DR InParanoid; P56718; -.
DR OMA; VMECRSV; -.
DR OrthoDB; 981089at2759; -.
DR PhylomeDB; P56718; -.
DR TreeFam; TF315303; -.
DR Reactome; R-RNO-389397; Orexin and neuropeptides FF and QRFP bind to their respective receptors.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:P56718; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000013838; Expressed in brain and 3 other tissues.
DR Genevisible; P56718; RN.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0016499; F:orexin receptor activity; IDA:RGD.
DR GO; GO:0017046; F:peptide hormone binding; IDA:RGD.
DR GO; GO:0007631; P:feeding behavior; IEA:InterPro.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:RGD.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IDA:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:RGD.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; ISS:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000204; Orexin_rcpt.
DR InterPro; IPR004059; OX1R.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01521; OREXIN1R.
DR PRINTS; PR01064; OREXINR.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..416
FT /note="Orexin/Hypocretin receptor type 1"
FT /id="PRO_0000069987"
FT TOPO_DOM 1..46
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TRANSMEM 47..67
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TOPO_DOM 68..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TRANSMEM 83..105
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TOPO_DOM 106..119
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TRANSMEM 120..140
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TOPO_DOM 141..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TRANSMEM 161..182
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TOPO_DOM 183..213
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TRANSMEM 214..235
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TOPO_DOM 236..298
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TRANSMEM 299..321
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TOPO_DOM 322..336
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TRANSMEM 337..360
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT TOPO_DOM 361..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 26..41
FT /note="Required for response to orexin-A"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT SITE 36
FT /note="Important for responses to orexin"
FT /evidence="ECO:0000250|UniProtKB:O43613"
FT CARBOHYD 194
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 119..202
FT /evidence="ECO:0000250|UniProtKB:O43613"
SQ SEQUENCE 416 AA; 46800 MW; 774DE7A22EA05D18 CRC64;
MEPSATPGAQ PGVPTSSGEP FHLPPDYEDE FLRYLWRDYL YPKQYEWVLI AAYVAVFLIA
LVGNTLVCLA VWRNHHMRTV TNYFIVNLSL ADVLVTAICL PASLLVDITE SWLFGHALCK
VIPYLQAVSV SVAVLTLSFI ALDRWYAICH PLLFKSTARR ARGSILGIWA VSLAVMVPQA
AVMECSSVLP ELANRTRLFS VCDERWADEL YPKIYHSCFF FVTYLAPLGL MGMAYFQIFR
KLWGPQIPGT TSALVRNWKR PSEQLEAQHQ GLCTEPQPRA RAFLAEVKQM RARRKTAKML
MVVLLVFALC YLPISVLNVL KRVFGMFRQA SDREAVYACF TFSHWLVYAN SAANPIIYNF
LSGKFREQFK AAFSCCLPGL GPSSSARHKS LSLQSRCSVS KVSEHVVLTT VTTVLS
