OX2R_PIG
ID OX2R_PIG Reviewed; 444 AA.
AC O62809; Q2MK76;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 07-SEP-2016, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Orexin receptor type 2;
DE Short=Ox-2-R;
DE Short=Ox2-R;
DE Short=Ox2R;
DE AltName: Full=Hypocretin receptor type 2;
GN Name=HCRTR2;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1] {ECO:0000312|EMBL:ABC59144.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Hypothalamus {ECO:0000312|EMBL:ABC59144.1};
RA Bin Y.F.;
RT "Molecular cloning of the porcine type 2 orexin receptor.";
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Duroc;
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 106-182.
RC TISSUE=Hypothalamus;
RA Matteri R.L., Dyer C.J.;
RT "Partial cDNA sequence of the porcine type 2 orexin receptor.";
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nonselective, high-affinity receptor for both orexin-A and
CC orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+)
CC levels in response to orexin-A binding. {ECO:0000250|UniProtKB:O43614}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O43614};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:O43614}.
CC -!- DOMAIN: The N-terminal region is required for orexin signaling.
CC {ECO:0000250|UniProtKB:O43614}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ321702; ABC59144.1; -; mRNA.
DR EMBL; CU694214; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF059740; AAC16044.1; -; mRNA.
DR RefSeq; NP_001123423.1; NM_001129951.1.
DR RefSeq; XP_013833190.1; XM_013977736.1.
DR AlphaFoldDB; O62809; -.
DR SMR; O62809; -.
DR STRING; 9823.ENSSSCP00000001611; -.
DR PaxDb; O62809; -.
DR Ensembl; ENSSSCT00000001653; ENSSSCP00000001611; ENSSSCG00000001481.
DR Ensembl; ENSSSCT00005071957; ENSSSCP00005044929; ENSSSCG00005044676.
DR Ensembl; ENSSSCT00015013430; ENSSSCP00015005195; ENSSSCG00015010253.
DR Ensembl; ENSSSCT00025088090; ENSSSCP00025038439; ENSSSCG00025064242.
DR Ensembl; ENSSSCT00030010512; ENSSSCP00030004549; ENSSSCG00030007812.
DR Ensembl; ENSSSCT00040027657; ENSSSCP00040011658; ENSSSCG00040020515.
DR Ensembl; ENSSSCT00045010613; ENSSSCP00045007231; ENSSSCG00045006393.
DR Ensembl; ENSSSCT00050012854; ENSSSCP00050005326; ENSSSCG00050009534.
DR Ensembl; ENSSSCT00055023881; ENSSSCP00055018895; ENSSSCG00055012193.
DR Ensembl; ENSSSCT00060019648; ENSSSCP00060007979; ENSSSCG00060014807.
DR Ensembl; ENSSSCT00065088292; ENSSSCP00065038617; ENSSSCG00065064319.
DR Ensembl; ENSSSCT00070037505; ENSSSCP00070031355; ENSSSCG00070019003.
DR GeneID; 397428; -.
DR KEGG; ssc:397428; -.
DR CTD; 3062; -.
DR VGNC; VGNC:88808; HCRTR2.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01020000230390; -.
DR InParanoid; O62809; -.
DR OMA; AEVYPKM; -.
DR OrthoDB; 981089at2759; -.
DR TreeFam; TF315303; -.
DR Proteomes; UP000008227; Chromosome 7.
DR Proteomes; UP000314985; Chromosome 7.
DR Bgee; ENSSSCG00000001481; Expressed in medulla oblongata and 9 other tissues.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0016499; F:orexin receptor activity; ISS:UniProtKB.
DR GO; GO:0022410; P:circadian sleep/wake cycle process; IEA:InterPro.
DR GO; GO:0007631; P:feeding behavior; IEA:InterPro.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0040011; P:locomotion; IEA:Ensembl.
DR GO; GO:0007218; P:neuropeptide signaling pathway; ISS:UniProtKB.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0010840; P:regulation of circadian sleep/wake cycle, wakefulness; ISS:UniProtKB.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; ISS:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000204; Orexin_rcpt.
DR InterPro; IPR004060; Orexin_rcpt_2.
DR Pfam; PF00001; 7tm_1; 1.
DR Pfam; PF03827; Orexin_rec2; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01522; OREXIN2R.
DR PRINTS; PR01064; OREXINR.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..444
FT /note="Orexin receptor type 2"
FT /id="PRO_0000069991"
FT TOPO_DOM 1..54
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TRANSMEM 55..75
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TOPO_DOM 76..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TRANSMEM 89..110
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TOPO_DOM 111..127
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TRANSMEM 128..150
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TOPO_DOM 151..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TRANSMEM 171..191
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TOPO_DOM 192..222
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TRANSMEM 223..243
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TOPO_DOM 244..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TRANSMEM 305..326
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TOPO_DOM 327..342
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TRANSMEM 343..366
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT TOPO_DOM 367..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..49
FT /note="Required for response to orexin-A"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT COMPBIAS 1..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 44
FT /note="Important for responses to orexin"
FT /evidence="ECO:0000250|UniProtKB:O43614"
FT CARBOHYD 14
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 22
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 202
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 127..210
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 181
FT /note="C -> Y (in Ref. 3; AAC16044)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 444 AA; 50765 MW; 29C05AC3EBA9201A CRC64;
MSGTKLEDSP PCRNWSSAPE LNETQEPFLN PTDYDDEEFL RYLWREYLHP KEYEWVLIAG
YIIVFVVALI GNVLVCVAVW KNHHMRTVTN YFIVNLSLAD VLVTITCLPA TLVVDITETW
FFGQSLCKVI PYLQTVSVSV SVLTLSCIAL DRWYAICHPL MFKSTAKRAR NSIVIIWIVS
CIIMIPQAIV MECSTMLPGL ANKTTLFTVC DERWGGEIYP KMYHICFFLV TYMAPLCLMV
LAYLQIFRKL WCRQIPGTSS VVQRKWKPLQ PVSQPRGPGQ QTKSRISAVA AEIKQIRARR
KTARMLMVVL LVFAICYLPI SILNVLKRVF GMFTHTEDRE TVYAWFTFSH WLVYANSAAN
PIIYNFLSGK FREEFKAAFS CCCLGVHHRQ EDRLARGRTS TESRKSLTTQ ISNFDNISKL
SEQVLLTSMS TLPAANGAGQ LQNW
