ID   OXDA_TRIVR              Reviewed;         356 AA.
AC   Q99042;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=D-amino-acid oxidase;
DE            Short=DAAO;
DE            Short=DAMOX;
DE            Short=DAO;
DE            EC=1.4.3.3;
GN   Name=DAO1;
OS   Trigonopsis variabilis (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Trigonopsidaceae; Trigonopsis.
OX   NCBI_TaxID=34364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CBS 4095;
RX   PubMed=9434346;
RX   DOI=10.1002/(sici)1097-0061(199712)13:15<1399::aid-yea187>3.0.co;2-7;
RA   Gonzalez F.J., Montes J., Martin F., Lopez M.C., Ferminan E., Catalan J.,
RA   Galan M.A., Dominguez A.;
RT   "Molecular cloning of TvDAO1, a gene encoding a D-amino acid oxidase from
RT   Trigonopsis variabilis and its expression in Saccharomyces cerevisiae and
RT   Kluyveromyces lactis.";
RL   Yeast 13:1399-1408(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a D-alpha-amino acid + H2O + O2 = a 2-oxocarboxylate + H2O2 +
CC         NH4(+); Xref=Rhea:RHEA:21816, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:28938, ChEBI:CHEBI:35179,
CC         ChEBI:CHEBI:59871; EC=1.4.3.3;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SIMILARITY: Belongs to the DAMOX/DASOX family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z50019; CAA90322.1; -; Genomic_DNA.
DR   PIR; S39437; S39437.
DR   AlphaFoldDB; Q99042; -.
DR   SMR; Q99042; -.
DR   PRIDE; Q99042; -.
DR   BRENDA; 1.4.3.3; 6488.
DR   SABIO-RK; Q99042; -.
DR   GO; GO:0003884; F:D-amino-acid oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0046416; P:D-amino acid metabolic process; IEA:InterPro.
DR   InterPro; IPR006181; D-amino_acid_oxidase_CS.
DR   InterPro; IPR023209; DAO.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   PANTHER; PTHR11530; PTHR11530; 1.
DR   Pfam; PF01266; DAO; 1.
DR   PIRSF; PIRSF000189; D-aa_oxidase; 1.
DR   PROSITE; PS00677; DAO; 1.
PE   3: Inferred from homology;
KW   FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN           1..356
FT                   /note="D-amino-acid oxidase"
FT                   /id="PRO_0000162768"
FT   BINDING         4..18
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         32..33
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         43..44
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         48..50
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         171
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         194
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         243
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         302
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         329..333
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         330
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   356 AA;  39301 MW;  A2935E9DB71A49FC CRC64;
     MAKIVVIGAG VAGLTTALQL LRKGHEVTIV SEFTPGDLSI GYTSPWAGAN WLTFYDGGKL
     ADYDAVSYPI LRELARSSPE AGIRLISQRS HVLKRDLPKL EVAMSAICQR NPWFKNTVDS
     FEIIEDRSRI VHDDVAYLVE FRSVCIHTGV YLNWLMSQCL SLGATVVKRR VNHIKDANLL
     HSSGSRPDVI VNCSGLFARF LGGVEDKKMY PIRGQVVLVR NSLPFMASFS STPEKENEDE
     ALYIMTRFDG TSIIGGCFQP NNWSSEPDPS LTHRILSRAL DRFPELTKDG PLDIVRECVG
     HRPGREGGPR VELEKIPGVG FVVHNYGAAG AGYQSSYGMA DEAVSYVERA LTRPNL