OXLA_NEUCR
ID OXLA_NEUCR Reviewed; 696 AA.
AC P23623; Q7RVE5; Q8X0D4;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 30-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=L-amino-acid oxidase;
DE Short=LAAO;
DE Short=LAO;
DE EC=1.4.3.2;
DE Flags: Precursor;
GN Name=lox; ORFNames=B14A6.230, NCU01066;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=2145270; DOI=10.1016/s0021-9258(17)44895-2;
RA Niedermann D.M., Lerch K.;
RT "Molecular cloning of the L-amino-acid oxidase gene from Neurospora
RT crassa.";
RL J. Biol. Chem. 265:17246-17251(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an L-alpha-amino acid + H2O + O2 = a 2-oxocarboxylate + H2O2 +
CC NH4(+); Xref=Rhea:RHEA:13781, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:28938, ChEBI:CHEBI:35179,
CC ChEBI:CHEBI:59869; EC=1.4.3.2;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC -!- INDUCTION: By addition of L-amino acids after nitrogen starvation, by
CC starvation in phosphate buffer and by the addition of protein synthesis
CC inhibitors, D-amino acids, or ATP.
CC -!- SIMILARITY: Belongs to the flavin monoamine oxidase family.
CC {ECO:0000305}.
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DR EMBL; AL670007; CAD21325.1; -; Genomic_DNA.
DR EMBL; CM002240; EAA32442.1; -; Genomic_DNA.
DR PIR; A38314; A38314.
DR RefSeq; XP_961678.1; XM_956585.2.
DR AlphaFoldDB; P23623; -.
DR SMR; P23623; -.
DR STRING; 5141.EFNCRP00000004415; -.
DR PRIDE; P23623; -.
DR EnsemblFungi; EAA32442; EAA32442; NCU01066.
DR GeneID; 3877869; -.
DR KEGG; ncr:NCU01066; -.
DR VEuPathDB; FungiDB:NCU01066; -.
DR HOGENOM; CLU_004498_8_2_1; -.
DR InParanoid; P23623; -.
DR OMA; YTHAWIA; -.
DR Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR GO; GO:0001716; F:L-amino-acid oxidase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0046592; F:polyamine oxidase activity; IBA:GO_Central.
DR GO; GO:0009063; P:cellular amino acid catabolic process; IBA:GO_Central.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; FAD; Flavoprotein; Oxidoreductase;
KW Reference proteome; Zymogen.
FT PROPEP 1..130
FT /id="PRO_0000001708"
FT CHAIN 131..696
FT /note="L-amino-acid oxidase"
FT /id="PRO_0000001709"
FT BINDING 207
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 215
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 236..237
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 237
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 440
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 564
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 649
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT BINDING 658..661
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT CONFLICT 6..13
FT /note="AAGAALLA -> RGCGTAR (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 53
FT /note="I -> L (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 343
FT /note="F -> L (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 391..414
FT /note="SASAHSYWDTLYEGMYFSASTWKT -> APALTRTGTRCTKGCTFPRRRGA
FT (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 440
FT /note="V -> L (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 684..685
FT /note="AT -> GRP (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 696 AA; 77013 MW; 3909C59198E96008 CRC64;
MKWSAAAGAA LLALPANSAV TASLPLKLET RSSLNSRLSN IHVERSASVE GAISYTYGSC
QAKREEEAHH SISQPTDAHH DRLVWVIPEN VQSGGCISAW SRANGRLVGR SRPQSFDFKS
IKMRRDLKAR ATKPSDSVAI HMTTDNGINP WGPWFDGVKL LEDKEISTVD VEKAKSKNIA
IVGAGMSGLM TYLCLTQAGM TNVSIIEGGN RLGGRVHTEY LSGGPFDYSY QEMGPMRFPN
TITLGNETYN VSDHQLVFQL AEEMNSLNGH SKNLSVDFIP WYQSNSNGLY YYDGIKNPET
GLPPTLAELA ANSSLALTRV SNNSTKSLSQ KVDAFLPDTD KFFAEMAQNM FKAHADWLSG
GLAGLPGDQW SEFGFMVNYL RGSLNDTAFL SASAHSYWDT LYEGMYFSAS TWKTIDGGLN
RLPLSFHPLV DNATTLNRRV ERVAFDAETQ KVTLHSRNSY KDSFESSEHD YAVIAAPFSI
VKKWRFSPAL DLTAPTLANA IQNLEYTSAC KVALEFRTRF WEHLPQPIYG SCSTTSDIPG
IGSICYPSYN INGTDGPASI LASYISGADW GDRWVSTPEE EHVQYVLNAM AEIHGEELVK
EQYTGQFNRR CWALDPLESA SWASPTVGQH ELYLPEYFQT RNNLVFVGEH TSYTHAWIAS
ALESGIRGSV QLLLELGLVD EAKATVDKWM ARWIDV
