ID   OXSC_CUCPE              Reviewed;         759 AA.
AC   Q6BE23;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Probable oxidosqualene cyclase;
DE            EC=5.4.99.-;
GN   Name=CPR;
OS   Cucurbita pepo (Vegetable marrow) (Summer squash).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX   NCBI_TaxID=3663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   DOI=10.1016/j.tet.2004.04.088;
RA   Shibuya M., Adachi S., Ebizuka Y.;
RT   "Cucurbitadienol synthase, the first committed enzyme for cucurbitacin
RT   biosynthesis, is a distinct enzyme from cycloartenol synthase for
RT   phytosterol biosynthesis.";
RL   Tetrahedron 60:6995-7003(2004).
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB116239; BAD34646.1; -; mRNA.
DR   AlphaFoldDB; Q6BE23; -.
DR   SMR; Q6BE23; -.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0031559; F:oxidosqualene cyclase activity; IEA:UniProt.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Repeat.
FT   CHAIN           1..759
FT                   /note="Probable oxidosqualene cyclase"
FT                   /id="PRO_0000412986"
FT   REPEAT          146..187
FT                   /note="PFTB 1"
FT   REPEAT          437..483
FT                   /note="PFTB 2"
FT   REPEAT          513..554
FT                   /note="PFTB 3"
FT   REPEAT          590..630
FT                   /note="PFTB 4"
FT   REPEAT          639..680
FT                   /note="PFTB 5"
FT   ACT_SITE        484
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   759 AA;  86954 MW;  3E8E16362A1F82EA CRC64;
     MWTLKFSKGW ETSDNAHLGR QFWEFDPNLQ PSLEEQARVH NVCNDFYTHR FQAKHSSDLL
     MRLQLKKANG SEVKLPTQVK LRSEEEMSEE AVETTLRRAI RFYSTMQTQD GFWPGDYAGP
     LFLLPGLVIG LSVTKALDTV LSRHHQQEMR RYLYNHQNED GGWGLHIEGN STMLCTALSY
     VSLRLLGEEM DGCDGALRQA RRWILDRGGA TSIPSWGKLW LSVLGVYEWE GNNPLPPEIW
     LLPYFLPLHP GRMWCHSRMV YLPMSYLYGK RFVGPISPII TSLRQELYTS PYHMIDWNLS
     RSLCAKEDLY TPHSKIQDML WDSIHKLGEP LLKKWPLSKL RQKALDFVIK HIHYEDENTH
     YLCLGPVSKV VNMVCCWDED PNSEAFTRHI SRIKDYLWLA EDGMKMQGYH GSQLWDVAFA
     IQAIVATDLV EEYGSVLKKA HDFVKNSQVR RNGFGDDDPS DWYRHNSKGG WPFSTPDNAW
     PVSDCTSEAL KVAIMMSQMP PTMVGEPMDI RKLYDAVDLI LSLQNSNGGF ASYELTRSHP
     WLETLNPAEI FGDVMIDYQY VECSSAAIER LKAFMKLHPS YRKKEIQACM AKAADFIETI
     QQPDGSWYGS WGICYTYGTW FGIKGLVACG RTYENSKTLR KATHFLLSKQ LKSGGWGESY
     LSAHNKVYTD LKNGKSHIVN TSWALLALIK AGQAQRDPSP LHQAATVLIN SQLDNGDFPQ
     QEIIGVFNKS CTISYSAYRN IFPIWALGEY QLKVLKRQE