ID   P0_PLRV1                Reviewed;         247 AA.
AC   P17518;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Suppressor of silencing P0;
DE   AltName: Full=28 kDa protein;
DE   AltName: Full=Protein ORF0;
GN   ORFNames=ORF0;
OS   Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Sobelivirales; Solemoviridae; Polerovirus.
OX   NCBI_TaxID=12046;
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2732710; DOI=10.1099/0022-1317-70-5-1037;
RA   Mayo M.A., Robinson D.J., Jolly C.A., Hyman L.;
RT   "Nucleotide sequence of potato leafroll luteovirus RNA.";
RL   J. Gen. Virol. 70:1037-1051(1989).
RN   [2]
RP   FUNCTION, MUTAGENESIS OF 59-LEU-PRO-60; 76-LEU-PRO-77; 87-TRP-GLY-88 AND
RP   TRP-140, AND DOMAIN.
RC   STRAIN=PLRV-IM;
RX   PubMed=24450775; DOI=10.1094/mpmi-08-13-0231-r;
RA   Zhuo T., Li Y.Y., Xiang H.Y., Wu Z.Y., Wang X.B., Wang Y., Zhang Y.L.,
RA   Li D.W., Yu J.L., Han C.G.;
RT   "Amino acid sequence motifs essential for P0-mediated suppression of RNA
RT   silencing in an isolate of potato leafroll virus from Inner Mongolia.";
RL   Mol. Plant Microbe Interact. 27:515-527(2014).
RN   [3]
RP   MUTAGENESIS OF LEU-76; TRP-87 AND PHE-220, AND FUNCTION.
RX   PubMed=30791535; DOI=10.3390/v11020170;
RA   Rashid M.O., Zhang X.Y., Wang Y., Li D.W., Yu J.L., Han C.G.;
RT   "The Three Essential Motifs in P0 for Suppression of RNA Silencing Activity
RT   of Potato leafroll virus Are Required for Virus Systemic Infection.";
RL   Viruses 11:0-0(2019).
RN   [4]
RP   FUNCTION.
RX   PubMed=31758809; DOI=10.1111/pce.13684;
RA   Patton M.F., Bak A., Sayre J.M., Heck M.L., Casteel C.L.;
RT   "A polerovirus, Potato leafroll virus, alters plant-vector interactions
RT   using three viral proteins.";
RL   Plant Cell Environ. 43:387-399(2020).
CC   -!- FUNCTION: Suppressor of RNA-mediated gene silencing, also known as
CC       post-transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (PubMed:24450775,
CC       PubMed:30791535). The P0 protein suppresses local PTGS using its F-box-
CC       like domain to mediate destabilization and degradation of the AGO1
CC       protein, although not via an interaction with host SKP1A
CC       (PubMed:24450775). Participates, together with the proteins P1 and P7,
CC       in the inhibition of the induction of aphid-induced host phytohormones
CC       (PubMed:31758809). This could play a role in the attraction to the
CC       infected plants by aphids (PubMed:31758809).
CC       {ECO:0000269|PubMed:24450775, ECO:0000269|PubMed:30791535,
CC       ECO:0000269|PubMed:31758809}.
CC   -!- DOMAIN: The F-box-like domain is required for suppression of host RNA
CC       silencing. {ECO:0000269|PubMed:24450775}.
CC   -!- SIMILARITY: Belongs to the polerovirus P0 protein family.
CC       {ECO:0000305}.
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DR   EMBL; D00530; BAA00416.1; -; Genomic_RNA.
DR   PIR; JA0117; WMVQ28.
DR   RefSeq; NP_056746.1; NC_001747.1.
DR   GeneID; 1493890; -.
DR   KEGG; vg:1493890; -.
DR   Proteomes; UP000006723; Genome.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR006755; Virus_P0.
DR   Pfam; PF04662; Luteo_PO; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Reference proteome; Suppressor of RNA silencing.
FT   CHAIN           1..247
FT                   /note="Suppressor of silencing P0"
FT                   /id="PRO_0000222393"
FT   DOMAIN          76..95
FT                   /note="F-box-like"
FT                   /evidence="ECO:0000269|PubMed:24450775"
FT   MUTAGEN         59..60
FT                   /note="LP->AA: No effect on the systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:24450775"
FT   MUTAGEN         76..77
FT                   /note="LP->AA: Complete loss of systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:24450775"
FT   MUTAGEN         76
FT                   /note="L->F: Complete loss of systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:30791535"
FT   MUTAGEN         87..88
FT                   /note="WG->AA: Complete loss of systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:24450775"
FT   MUTAGEN         87..88
FT                   /note="WG->GW: Complete loss of systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:24450775"
FT   MUTAGEN         87
FT                   /note="W->R: Complete loss of systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:30791535"
FT   MUTAGEN         140
FT                   /note="W->A: Almost complete loss of systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:24450775"
FT   MUTAGEN         220
FT                   /note="F->R: Complete loss of systemic RNA silencing
FT                   suppressor activity."
FT                   /evidence="ECO:0000269|PubMed:30791535"
SQ   SEQUENCE   247 AA;  28131 MW;  02E900959E8F0CEE CRC64;
     MIVLTQSGTL LFDQRFKLSK FLFVVIATGF PLLLQQASLI YGYNHEQIYR ICRSFLHVLP
     LLNCKRGRIS TSGLQLPRHL HYECLEWGLL CGTHPAIQIV GLTIVIKLDD PTTAAAYRSE
     LLRVSSSSYI QNAAGLSNGW GHDMEAFVRN AICLLELRER SIPQSGLRDL MGNYQHLVRS
     LLDACKVDHF VPLDFQHRSL MLNFARLYNQ LDLQGRAKSF RALTGFPVYV PSEDYLEGSF
     LQKELQE