ID   P0_TYYVF                Reviewed;         249 AA.
AC   P09504;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Suppressor of silencing P0;
DE   AltName: Full=29 kDa protein;
DE   AltName: Full=Protein ORF0;
GN   ORFNames=ORF0;
OS   Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Sobelivirales; Solemoviridae; Polerovirus.
OX   NCBI_TaxID=12043;
OH   NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH   NCBI_TaxID=3709; Brassica napus subsp. rapifera.
OH   NCBI_TaxID=138011; Brassica napus var. napus.
OH   NCBI_TaxID=3710; Brassica nigra (Black mustard) (Sinapis nigra).
OH   NCBI_TaxID=3715; Brassica oleracea var. botrytis (Cauliflower).
OH   NCBI_TaxID=3716; Brassica oleracea var. capitata (Cabbage).
OH   NCBI_TaxID=51350; Brassica rapa subsp. rapa (Turnip).
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH   NCBI_TaxID=3654; Citrullus lanatus (Watermelon) (Citrullus vulgaris).
OH   NCBI_TaxID=3721; Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=4232; Helianthus annuus (Common sunflower).
OH   NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH   NCBI_TaxID=103529; Phlox drummondii (Annual phlox).
OH   NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH   NCBI_TaxID=3726; Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH   NCBI_TaxID=3900; Trifolium subterraneum (Subterranean clover).
OH   NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3194229; DOI=10.1093/nar/16.21.9917;
RA   Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E.,
RA   Jonard G.;
RT   "Nucleotide sequence of beet western yellows virus RNA.";
RL   Nucleic Acids Res. 16:9917-9932(1988).
RN   [2]
RP   RETRACTED PAPER.
RX   PubMed=12050394; DOI=10.1128/jvi.76.13.6815-6824.2002;
RA   Pfeffer S., Dunoyer P., Heim F., Richards K.E., Jonard G.,
RA   Ziegler-Graff V.;
RT   "P0 of beet Western yellows virus is a suppressor of posttranscriptional
RT   gene silencing.";
RL   J. Virol. 76:6815-6824(2002).
RN   [3]
RP   RETRACTION NOTICE OF PUBMED:12050394.
RX   PubMed=29611664; DOI=10.1128/jvi.00022-17;
RA   Pfeffer S., Dunoyer P., Heim F., Richards K.E., Jonard G.,
RA   Ziegler-Graff V.;
RL   J. Virol. 91:0-0(2017).
RN   [4]
RP   FUNCTION, INTERACTION WITH ARABIDOPSIS THALIANA ASK1 AND ASK2, AND
RP   IDENTIFICATION IN A SCF P0 COMPLEX.
RX   PubMed=16446454; DOI=10.1073/pnas.0510784103;
RA   Pazhouhandeh M., Dieterle M., Marrocco K., Lechner E., Berry B., Brault V.,
RA   Hemmer O., Kretsch T., Richards K.E., Genschik P., Ziegler-Graff V.;
RT   "F-box-like domain in the polerovirus protein P0 is required for silencing
RT   suppressor function.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:1994-1999(2006).
RN   [5]
RP   FUNCTION, AND INTERACTION WITH ARABIDOPSIS THALIANA AGO1.
RX   PubMed=17869110; DOI=10.1016/j.cub.2007.08.039;
RA   Baumberger N., Tsai C.-H., Lie M., Havecker E., Baulcombe D.C.;
RT   "The Polerovirus silencing suppressor P0 targets ARGONAUTE proteins for
RT   degradation.";
RL   Curr. Biol. 17:1609-1614(2007).
CC   -!- FUNCTION: Suppressor of RNA-mediated gene silencing, also known as
CC       post-transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs. The P0 protein
CC       suppresses local PTGS using its F-box-like domain to mediate
CC       destabilization and degradation of the AGO1 protein.
CC       {ECO:0000269|PubMed:16446454, ECO:0000269|PubMed:17869110}.
CC   -!- SUBUNIT: Interacts (via F-box-like domain) with host AGO1; this
CC       interaction targets AGO1 for degradation, and thereby suppresses the
CC       silencing function of the latter. Interacts (via F-box-like domain)
CC       with host ASK1 and ASK2 (SKP proteins); these interactions are
CC       essential for viral pathogenicity. Part of a SCF P0 complex composed of
CC       P0 and the host proteins SKP and CUL1. {ECO:0000269|PubMed:16446454,
CC       ECO:0000269|PubMed:17869110}.
CC   -!- SIMILARITY: Belongs to the polerovirus P0 protein family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The publication has been retracted because some figures
CC       presented signs of inappropriate manipulation.
CC       {ECO:0000305|PubMed:29611664}.
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DR   EMBL; X13063; CAA31462.1; -; Genomic_RNA.
DR   PIR; S01938; S01938.
DR   RefSeq; NP_620484.1; NC_003743.1.
DR   GeneID; 940485; -.
DR   KEGG; vg:940485; -.
DR   Proteomes; UP000007545; Genome.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR006755; Virus_P0.
DR   Pfam; PF04662; Luteo_PO; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Reference proteome; Suppressor of RNA silencing.
FT   CHAIN           1..249
FT                   /note="Suppressor of silencing P0"
FT                   /id="PRO_0000222392"
FT   DOMAIN          63..67
FT                   /note="F-box-like"
SQ   SEQUENCE   249 AA;  29077 MW;  81A342BE56DE26FD CRC64;
     MQFLAHDNFH TLQVKKVRFL HPQQEVFLLA GLLLNIKQFV RAIKERNNVF KIDVFLRSLL
     YQLPFHLGSC FHDAPRELIP ATEPELCAWF SLQTGYAPAS TSGRVNLHVP GTKTSRRRII
     QRSFASDFSE KLKRFPECLF VSLELFQRLL STWTKDVERR IFFSCREIPL GSDTLMELAN
     LGEFLRVMVV GEQFHNSRLL SRLAVHCYKI YGEDGFISFW RIANLDHFDC FLTPEEILFS
     SSVYTEMFV