P1_PEMVW
ID P1_PEMVW Reviewed; 760 AA.
AC Q84710;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Protein P1;
DE AltName: Full=Genome-linked protein precursor;
DE AltName: Full=ORF1 protein;
DE Contains:
DE RecName: Full=Serine protease;
DE EC=3.4.21.-;
DE Contains:
DE RecName: Full=VPg;
DE Contains:
DE RecName: Full=P1-C25;
DE Flags: Precursor;
GN ORFNames=ORF1;
OS Pea enation mosaic virus-1 (strain WSG) (PEMV-1).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Sobelivirales; Solemoviridae; Enamovirus.
OX NCBI_TaxID=693989;
OH NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH NCBI_TaxID=3859; Lathyrus odoratus (Sweet pea).
OH NCBI_TaxID=3864; Lens culinaris (Lentil) (Cicer lens).
OH NCBI_TaxID=70936; Medicago arabica.
OH NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH NCBI_TaxID=60916; Trifolium incarnatum (Crimson clover).
OH NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
OH NCBI_TaxID=3908; Vicia sativa (Spring vetch) (Tare).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1875194; DOI=10.1099/0022-1317-72-8-1819;
RA Demler S.A., de Zoeten G.A.;
RT "The nucleotide sequence and luteovirus-like nature of RNA 1 of an aphid
RT non-transmissible strain of pea enation mosaic virus.";
RL J. Gen. Virol. 72:1819-1834(1991).
RN [2]
RP CHARACTERIZATION OF VPG, AND PROTEIN SEQUENCE.
RX PubMed=9714253; DOI=10.1099/0022-1317-79-8-2023;
RA Wobus C.E., Skaf J.S., Schultz M.H., de Zoeten G.A.;
RT "Sequencing, genomic localization and initial characterization of the VPg
RT of pea enation mosaic enamovirus.";
RL J. Gen. Virol. 79:2023-2025(1998).
CC -!- FUNCTION: Precursor from which the VPg molecule is probably released at
CC the onset of the RNA synthesis. Essential for virus replication (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Ribosomal frameshifting; Named isoforms=2;
CC Name=Protein P1;
CC IsoId=Q84710-1; Sequence=Displayed;
CC Name=RNA-directed RNA polymerase;
CC IsoId=P29154-1; Sequence=External;
CC -!- DOMAIN: The C-terminus part of protein P1 and P1-C25 displays RNA-
CC binding properties. {ECO:0000250}.
CC -!- PTM: Specific enzymatic cleavages in vivo yield mature proteins. The
CC protease probably cleaves itself and releases the VPg protein (By
CC similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform Protein P1]: Produced by conventional
CC translation.
CC -!- SIMILARITY: Belongs to the peptidase S39B family. {ECO:0000305}.
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DR EMBL; L04573; AAA72300.1; -; Genomic_RNA.
DR PIR; JQ1383; JQ1383.
DR RefSeq; NP_619736.1; NC_003629.1.
DR GeneID; 940254; -.
DR KEGG; vg:940254; -.
DR Proteomes; UP000000519; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0070008; F:serine-type exopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0016032; P:viral process; IEA:InterPro.
DR InterPro; IPR018019; Luteovirus_Orf2.
DR InterPro; IPR000382; Peptidase_S39B_luteovirus.
DR Pfam; PF02122; Peptidase_S39; 1.
DR PRINTS; PR00913; LVIRUSORF2.
DR PROSITE; PS51868; PEPTIDASE_S39; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Hydrolase; Membrane; Protease;
KW Reference proteome; Ribosomal frameshifting; RNA-binding; Serine protease;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..760
FT /note="Protein P1"
FT /id="PRO_0000390915"
FT CHAIN 316..515
FT /note="Serine protease"
FT /evidence="ECO:0000255"
FT /id="PRO_0000390916"
FT CHAIN 516..543
FT /note="VPg"
FT /evidence="ECO:0000255"
FT /id="PRO_0000390917"
FT CHAIN 544..760
FT /note="P1-C25"
FT /evidence="ECO:0000255"
FT /id="PRO_0000391318"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 318..515
FT /note="Peptidase S39"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT REGION 572..672
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 684..760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 589..608
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 622..643
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 713..729
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 366
FT /note="For protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT ACT_SITE 396
FT /note="For protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT ACT_SITE 465
FT /note="For protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT SITE 315..316
FT /note="Cleavage; by viral serine protease"
FT /evidence="ECO:0000255"
FT SITE 515..516
FT /note="Cleavage; by viral serine protease"
FT /evidence="ECO:0000255"
FT SITE 543..544
FT /note="Cleavage"
FT /evidence="ECO:0000255"
SQ SEQUENCE 760 AA; 84187 MW; 731B581AF51FFD14 CRC64;
MASFLKPVNS QGLWLSLLLA ITYLFLLPSA GQSLDPSGIG LAAGCSQSQG GISSFAALPR
PCNDSVCTLP DLGWSCQRTA QDTANQQQSP FNHTGHFLTT SGWTWPNWTC SPSQCQLLIH
LPTWQIVKQD FLLLLKEWDL LTMCQRCSDL LTKTPGFILR FAGETLILVA NLIEFVLVSW
SLWLCSVLVY VAQAVPGKFL LYMAAFCTTF WAWPRETASS LIRIVTTPLT LIGFLNKTGI
GLISHCLALT WNMFMTWSLL PWVTLMKMMK ILITSSRVLT RSGRPKRTSS KSLKHKLKIS
RAIQKKQGKK TPVEERTIPG VQIKKLREDP PKGVILRCTD QFGDHVGYAS AVKLEKGQTG
IVLPIHVWTD TVYINGPNGK LKMADFTALY EVTNHDSLIM TSAMAGWGSI LGVRPRPLTT
IDAVKLKNYS LFTERDGKWY VQAAKCIAPA EGMFRVVSDT RPGDSGLPLF DMKMNVVAVH
RGTWPSERFP ENRAFAILPV PDLTSSSSPK FTGCETYSEA ETAYEMADNF SDGEEILIRT
KGQSYRTFIG SNKVALLSIR KLEEELSRGP IGLWADDTED DESAPRRSGN GLFRSTPEKQ
SQAKTPSPKV EESAAPPPAP RAEKVRHVRR SEMTPEQKRA DNLRRRKAKA AKKTPSTPPK
KSKDKAPTLS QVAELVEKAV RAALTVQPRR SRASSKISIG GRNPGRKPQV SIQLDPVPSQ
STSVPPKDSQ AGESAWLGPR RSYRPVQKST VGQKQEPRRN
