P1_TYYVF
ID P1_TYYVF Reviewed; 607 AA.
AC P09506;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Protein P1;
DE AltName: Full=66.2 kDa protein;
DE AltName: Full=Genome-linked protein precursor;
DE AltName: Full=Protein ORF1;
DE Contains:
DE RecName: Full=Serine protease;
DE EC=3.4.21.-;
DE Contains:
DE RecName: Full=VPg/P1-C25;
DE Flags: Precursor;
GN ORFNames=ORF1;
OS Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Sobelivirales; Solemoviridae; Polerovirus.
OX NCBI_TaxID=12043;
OH NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH NCBI_TaxID=3709; Brassica napus subsp. rapifera.
OH NCBI_TaxID=138011; Brassica napus var. napus.
OH NCBI_TaxID=3710; Brassica nigra (Black mustard) (Sinapis nigra).
OH NCBI_TaxID=3715; Brassica oleracea var. botrytis (Cauliflower).
OH NCBI_TaxID=3716; Brassica oleracea var. capitata (Cabbage).
OH NCBI_TaxID=51350; Brassica rapa subsp. rapa (Turnip).
OH NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH NCBI_TaxID=3654; Citrullus lanatus (Watermelon) (Citrullus vulgaris).
OH NCBI_TaxID=3721; Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica).
OH NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH NCBI_TaxID=4232; Helianthus annuus (Common sunflower).
OH NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH NCBI_TaxID=103529; Phlox drummondii (Annual phlox).
OH NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH NCBI_TaxID=3726; Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH NCBI_TaxID=3900; Trifolium subterraneum (Subterranean clover).
OH NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3194229; DOI=10.1093/nar/16.21.9917;
RA Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E.,
RA Jonard G.;
RT "Nucleotide sequence of beet western yellows virus RNA.";
RL Nucleic Acids Res. 16:9917-9932(1988).
CC -!- FUNCTION: Precursor from which the VPg molecule is probably released at
CC the onset of the RNA synthesis. Essential for virus replication (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Protein P1]: Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Ribosomal frameshifting; Named isoforms=2;
CC Name=Protein P1;
CC IsoId=P09506-1; Sequence=Displayed;
CC Name=RNA-directed RNA polymerase;
CC IsoId=P09507-1; Sequence=External;
CC -!- DOMAIN: The C-terminus part of protein P1 and VPg/P1-C25 displays RNA-
CC binding properties. {ECO:0000250}.
CC -!- PTM: Specific enzymatic cleavages in vivo yield mature proteins. The
CC protease probably cleaves itself and releases the VPg protein (By
CC similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform Protein P1]: Produced by conventional
CC translation.
CC -!- SIMILARITY: Belongs to the peptidase S39B family. {ECO:0000305}.
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DR EMBL; X13063; CAA31463.1; -; Genomic_RNA.
DR PIR; S01939; S01939.
DR RefSeq; NP_620486.1; NC_003743.1.
DR SMR; P09506; -.
DR MEROPS; S39.002; -.
DR GeneID; 940483; -.
DR KEGG; vg:940483; -.
DR Proteomes; UP000007545; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0070008; F:serine-type exopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0016032; P:viral process; IEA:InterPro.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR018019; Luteovirus_Orf2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR000382; Peptidase_S39B_luteovirus.
DR Pfam; PF02122; Peptidase_S39; 1.
DR PRINTS; PR00913; LVIRUSORF2.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS51868; PEPTIDASE_S39; 1.
PE 3: Inferred from homology;
KW Hydrolase; Membrane; Protease; Reference proteome; Ribosomal frameshifting;
KW RNA-binding; Serine protease; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..607
FT /note="Protein P1"
FT /id="PRO_0000222395"
FT CHAIN 204..400
FT /note="Serine protease"
FT /evidence="ECO:0000255"
FT /id="PRO_0000390897"
FT CHAIN 401..607
FT /note="VPg/P1-C25"
FT /evidence="ECO:0000255"
FT /id="PRO_0000390898"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 206..400
FT /note="Peptidase S39"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT REGION 457..485
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 517..607
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 465..484
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 560..579
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 254
FT /note="For protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT ACT_SITE 289
FT /note="For protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT ACT_SITE 357
FT /note="For protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT SITE 203..204
FT /note="Cleavage; by viral serine protease"
FT /evidence="ECO:0000255"
FT SITE 400..401
FT /note="Cleavage; by viral serine protease"
FT /evidence="ECO:0000250"
SQ SEQUENCE 607 AA; 66211 MW; 2A41F82911DEC84F CRC64;
MYSKLMFFFA LCSISFLFTS EAASTMLLES SYLPLNQSYA PGFLYKRDML PPPLQAVLTY
TCPEPRPLAE ESYNDLLRAI SQKSSSDFQN AYSLALSFSS DFYQHGLKTL KDVSFLAVEK
FLWGLTRLWS SLILASFSAL WWLVSNFTTP VFCLALLYTV TKYMVKTVSF LFGGLPIWII
SIAFSLLKKS FSALRSTPKC LYEKAIDGFK SFTIPQSPPK SCVIPITHAS GNHAGYASCI
KLYNGENALM TATHVLRDCP NAVAVSAKGL KTRIPLAEFK TIAKSDKGDV TLLRGPPNWE
GLLGCKAANV ITAANLAKCK ASIYSFDRDG WVSSYAEIVG SEGTDVMVLS HTEGGHSGSP
YFNGKTILGV HSGASATGNY NLMAPIPSLP GLTSPTYVFE TTAPQGRVFA QEDIAEIEGL
YAQVMKRVQQ AEDFKPKTGK YWGDMEDDED IFFESKEDLS GNGVRGTVRG TNGEGSSTPK
TSNVDGKEMM EKIISSLVGK INLENIERKV IEEISAKAMK TPKSRRRRAP KKQPESSKDT
SPRSTTGKYQ PPHVRSPASV TAANCPNTTT PSKKKNLAGG RPSSGTIPRW VRKQAASAGP
SSAPKQN
