ID   A2_BPT5                 Reviewed;         135 AA.
AC   P23541; Q6QGT1;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 2.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Protein A2;
GN   Name=A2; Synonyms=A2-3;
GN   ORFNames=T5.006 {ECO:0000312|EMBL:AAS77053.1},
GN   T5p006 {ECO:0000312|EMBL:AAU05303.1};
GN   and
GN   Name=A2.2; ORFNames=T5p164;
OS   Escherichia phage T5 (Enterobacteria phage T5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX   NCBI_TaxID=2695836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT   "Bacteriophage T5 complete genome.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11303-B5;
RX   PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA   Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA   Hu S.;
RT   "Complete genome sequence of bacteriophage T5.";
RL   Virology 332:45-65(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=St0 deletion mutant;
RX   PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA   Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA   Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA   Boulanger P.;
RT   "Insights into bacteriophage T5 structure from analysis of its
RT   morphogenesis genes and protein components.";
RL   J. Virol. 88:1162-1174(2014).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-29.
RX   PubMed=7049170; DOI=10.1016/0006-291x(82)91104-4;
RA   Fox J.W., Barish A., Snyder C.E. Jr., Benzinger R.;
RT   "Amino terminal sequence of the bacteriophage T5-coded gene A2 protein.";
RL   Biochem. Biophys. Res. Commun. 106:265-269(1982).
RN   [5]
RP   PROTEIN SEQUENCE OF 2-134, AND DNA-BINDING.
RX   PubMed=2059212; DOI=10.1016/0006-291x(91)90674-v;
RA   Snyder C.E. Jr.;
RT   "Amino acid sequence of the bacteriophage T5 gene A2 protein.";
RL   Biochem. Biophys. Res. Commun. 177:1240-1246(1991).
RN   [6]
RP   SUBUNIT.
RX   PubMed=5136582; DOI=10.1016/0022-2836(71)90155-0;
RA   Beckman L.D., Hoffman M.S., McCorquodale D.J.;
RT   "Pre-early proteins of bacteriophage T5: structure and function.";
RL   J. Mol. Biol. 62:551-564(1971).
RN   [7]
RP   INDUCTION.
RX   PubMed=325230; DOI=10.1128/jvi.22.2.480-488.1977;
RA   McCorquodale D.J., Shaw A.R., Shaw P.K., Chinnadurai G.;
RT   "Pre-early polypeptides of bacteriophages T5 and BF23.";
RL   J. Virol. 22:480-488(1977).
RN   [8]
RP   FUNCTION.
RX   PubMed=7033565; DOI=10.1128/jvi.40.3.958-962.1981;
RA   McCorquodale D.J., Chen C.W., Joseph M.K., Woychik R.;
RT   "Modification of RNA polymerase from Escherichia coli by pre-early gene
RT   products of bacteriophage T5.";
RL   J. Virol. 40:958-962(1981).
CC   -!- FUNCTION: Involved, together with A1 protein, in the second step
CC       transfer (SST) which allows the completion of viral DNA into the host
CC       cell. {ECO:0000269|PubMed:7033565}.
CC   -!- SUBUNIT: Interacts with A1 protein; the two proteins form
CC       heterooligomers. {ECO:0000269|PubMed:5136582}.
CC   -!- INDUCTION: Expressed pre-early in the viral replicative cycle.
CC       Expressed with a few other proteins from the first step transfer (FST)
CC       DNA. FST DNA corresponds to the first small portion of the genome that
CC       enters into the host cell at the beginning of infection before pausing.
CC       {ECO:0000269|PubMed:325230}.
CC   -!- MISCELLANEOUS: This gene is part of the long terminal repeats present
CC       at both ends of the viral genome and is thus duplicated.
CC       {ECO:0000269|PubMed:15661140}.
CC   -!- SIMILARITY: Belongs to the T5likevirus A2 protein family.
CC       {ECO:0000305}.
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DR   EMBL; AY543070; AAS77053.1; -; Genomic_DNA.
DR   EMBL; AY692264; AAU05159.1; -; Genomic_DNA.
DR   EMBL; AY692264; AAU05303.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX11944.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX12095.1; -; Genomic_DNA.
DR   PIR; JQ1034; WXBPT5.
DR   RefSeq; YP_006834.1; NC_005859.1.
DR   GeneID; 2777660; -.
DR   KEGG; vg:2777660; -.
DR   Proteomes; UP000002107; Genome.
DR   Proteomes; UP000002141; Genome.
DR   Proteomes; UP000002503; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Early protein; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2059212,
FT                   ECO:0000269|PubMed:7049170"
FT   CHAIN           2..135
FT                   /note="Protein A2"
FT                   /id="PRO_0000165289"
SQ   SEQUENCE   135 AA;  14331 MW;  F212B0EC5A367C3A CRC64;
     MTNAKTAKFA WNEENTQKAV SMYQQLINEN GLDFANSDGL KEIAKAVGAA SPVSVRSKLT
     SAKAYQKSDK PRKVGGGSSI RKAHYVRVIA KHAIDSGIIK DADDLASLES AKLETLDAVA
     QLLGVADEVK QAAGE