P230P_PLAF7
ID P230P_PLAF7 Reviewed; 2508 AA.
AC O96175; A0A144A0J8;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Male gametocyte surface protein P230p;
DE Flags: Precursor;
GN Name=PFS230P; Synonyms=PF230P, PfsMR5, S230P;
GN ORFNames=PF3D7_0208900, PFB0400w;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=9804551; DOI=10.1126/science.282.5391.1126;
RA Gardner M.J., Tettelin H., Carucci D.J., Cummings L.M., Aravind L.,
RA Koonin E.V., Shallom S.J., Mason T., Yu K., Fujii C., Pederson J., Shen K.,
RA Jing J., Aston C., Lai Z., Schwartz D.C., Pertea M., Salzberg S.L.,
RA Zhou L., Sutton G.G., Clayton R., White O., Smith H.O., Fraser C.M.,
RA Adams M.D., Venter J.C., Hoffman S.L.;
RT "Chromosome 2 sequence of the human malaria parasite Plasmodium
RT falciparum.";
RL Science 282:1126-1132(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [3]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=12106866; DOI=10.1016/s0166-6851(02)00091-9;
RA Eksi S., Williamson K.C.;
RT "Male-specific expression of the paralog of malaria transmission-blocking
RT target antigen Pfs230, PfB0400w.";
RL Mol. Biochem. Parasitol. 122:127-130(2002).
RN [4]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=30297725; DOI=10.1038/s41598-018-33236-x;
RA Marin-Mogollon C., van de Vegte-Bolmer M., van Gemert G.J., van Pul F.J.A.,
RA Ramesar J., Othman A.S., Kroeze H., Miao J., Cui L., Williamson K.C.,
RA Sauerwein R.W., Janse C.J., Khan S.M.;
RT "The Plasmodium falciparum male gametocyte protein P230p, a paralog of
RT P230, is vital for ookinete formation and mosquito transmission.";
RL Sci. Rep. 8:14902-14902(2018).
CC -!- FUNCTION: Plays an essential role in male gamete fertility (By
CC similarity). Required for the binding to erythrocytes and thus, for the
CC formation of exflagellation centers (By similarity).
CC {ECO:0000250|UniProtKB:W7K265}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000269|PubMed:12106866}. Cell
CC membrane {ECO:0000269|PubMed:12106866, ECO:0000269|PubMed:30297725};
CC Peripheral membrane protein {ECO:0000305}. Note=Present on the surface
CC of male gametocytes. {ECO:0000269|PubMed:12106866,
CC ECO:0000269|PubMed:30297725}.
CC -!- DEVELOPMENTAL STAGE: Specifically presents on the surface of stage V
CC male gametocytes (at protein level) (PubMed:12106866, PubMed:30297725).
CC Not expressed in gametes or asexual parasites (PubMed:12106866).
CC Expression peaks in stage III/IV gametocytes, then sharply declines in
CC gametes (PubMed:12106866). {ECO:0000269|PubMed:12106866,
CC ECO:0000269|PubMed:30297725}.
CC -!- MISCELLANEOUS: In P.berghei, dispensable for male gamete attachment to
CC erythrocytes and the formation of exflagellation centers.
CC {ECO:0000269|PubMed:30297725}.
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DR EMBL; LN999943; CZT98103.1; -; Genomic_DNA.
DR PIR; A71616; A71616.
DR RefSeq; XP_001349599.1; XM_001349563.1.
DR AlphaFoldDB; O96175; -.
DR SMR; O96175; -.
DR STRING; 5833.PFB0400w; -.
DR PRIDE; O96175; -.
DR EnsemblProtists; CZT98103; CZT98103; PF3D7_0208900.
DR GeneID; 812681; -.
DR KEGG; pfa:PF3D7_0208900; -.
DR VEuPathDB; PlasmoDB:PF3D7_0208900; -.
DR HOGENOM; CLU_230593_0_0_1; -.
DR InParanoid; O96175; -.
DR OMA; FYCTCED; -.
DR PhylomeDB; O96175; -.
DR Proteomes; UP000001450; Chromosome 2.
DR GO; GO:0009986; C:cell surface; IDA:GeneDB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.2860; -; 6.
DR InterPro; IPR010884; 6_CYS_dom.
DR InterPro; IPR038160; 6_CYS_dom_sf.
DR Pfam; PF07422; s48_45; 6.
DR SMART; SM00970; s48_45; 6.
DR PROSITE; PS51701; 6_CYS; 10.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Malaria; Membrane;
KW Reference proteome; Repeat; Signal.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..2508
FT /note="Male gametocyte surface protein P230p"
FT /id="PRO_0000423564"
FT DOMAIN 394..516
FT /note="6-Cys 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 676..800
FT /note="6-Cys 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 831..1096
FT /note="6-Cys 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 1099..1231
FT /note="6-Cys 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 1268..1428
FT /note="6-Cys 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 1433..1565
FT /note="6-Cys 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 1656..1804
FT /note="6-Cys 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 1807..1984
FT /note="6-Cys 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 2197..2354
FT /note="6-Cys 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DOMAIN 2357..2481
FT /note="6-Cys 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT REGION 2081..2113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2085..2110
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 230
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 254
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 362
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 414
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 601
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 674
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 703
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 779
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 849
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 986
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 995
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1065
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1074
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1231
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1385
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1525
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1550
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1567
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1750
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1755
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1788
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2016
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2047
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2143
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2239
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2255
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 443..493
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 680..700
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 714..775
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 725..773
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 835..856
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 871..1072
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1103..1129
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1144..1206
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1157..1204
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1272..1293
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1308..1404
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1437..1464
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1478..1547
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1488..1545
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1704..1782
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1811..1883
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1897..1966
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 1908..1964
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 2361..2386
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 2400..2461
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT DISULFID 2411..2459
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
SQ SEQUENCE 2508 AA; 293647 MW; 628E126648E40F1C CRC64;
MFIYLFIYLF FKMDKKRTFY YLFFFFTFLV YVLYFDNIKS VLRSSTKKKK KKKICKSTFY
VHEESEEIKS WLRNSNERDK GKKFFIFERL IKERKYICVN KYKRNNKLKW IYKNTYEKTK
NICDDYNILF KCIKGIIYDK NKETFFETFF ENFWDNIFYM NKYIFNIYYY MFDYTKKVKK
KIEGIKENMN IRHNNNYNNI FYVHKFFLFN DDEEKKKRND DIKINIKLHN NTRKLSVSEE
NVELKPYIKQ GERNETVVNL YEYFTGGVKR SNNNNEIVVT STEQFHRIVI ICFKPTVKHS
HIITSPHDAL NHIVEENDKI KLSEEIYSIP FYPIYGNLGL KNVITTGIVE FMIPYFSRTQ
MNFTVTCANG EMNDLYKFED LIKIRIRIPR NTKKILGLST NEKDKTVFER IVDNTSNEYR
FKSYNNKIVG IKLENSILDP PGCFKTVYED DKILQLEVFL QYVKCINLDR DNYKIRFFFL
PEDFGDEEIE FSCKFTYKKK TSKIIFGLGE TSVDKDIFYL EDEHVKLNIN QDISGDEPYY
SHLNYNGIPY NICNFQYKSE YDSQVCERTI HEFSLFIYNC DTLVGTQIQT TEPITSVKYL
NSTYPINKFS DITLLSKDID IEGLEEAFRN SKFFLTSYIN HGPFPLIIEC VISNSNKDYQ
NVYILLHLRT SIKNRSVSFC DFEKVQGYNY LNNYIDGKIC NINITSNSVF GFRCPSNSIK
EPKDCFSQVY IDKKVYKLND KLSNKLILYS MKQENLAIAG FNNYISNSFS FECYCIDKNQ
TYSSYERTSG EDIFNHIVKR IHVHYKNYDE LYDYNIHDKI TYEPIMKNPP ITYLCDFLNK
KQILQPLNNK TKNYICTIWY PKPLNYIALN CPTNRRDEQN DQTISEVYNS LQKDLLKPTG
IEQQIDQKKK ELNLLFNKRN IYSNLYHLPK NAPKRTINKN GLNIVNIDEI IPGILIKDVI
NMKLEDVIKP DLLTPTSFLH KTYNTNKSYL FSTRNKSTSV FNTPSIYTPL THTSFSISPK
SVPLTKSRIE ETHSSSNTYE QYIGKRNSIE NGFFIFQLPP YLKKNQTIEF ACINDSTIKN
KNVGNNGIMT IHLKSFGNPI EGCYFYKNSA KYNYLKKSIK IDDLKKEECT IRSDGEIEFV
GIMCPYENNL YLTPSSCFLK TYDNTDNLVE LLDINENFEY YSNDKGISYL KIPQEFLNHV
HLFCYCNVDK DSVSDTNVLV KKENKISLEL NYSNKGFNII KTIDYQYEAD ILIGYSYYFK
RVTPIYRKKH ICDFTTEDNS LEPESEDKMI YSCYLSLENN LNFIEVKCPK NKKSSNSEWL
FKYGTFDKSS EIMEDDENIK KYEHMKYMPE DKDEIIYLFK KQKLEDILPG VIIFDKNRYF
FEKGNFSFVT PLIVKEDVTI KLLCDNSETK IDDKIGKKGI ILIKIPQHIT DKKFYGCDFS
GDSNKKSSFY YTSVYDLKTQ NQYCEVKLKE NIIISLNCPN GNINPNNCFN NVFLKTNMNE
QIHEKIQNIF DQVKVINTKS HVLLNSSSTF LIISKITKKE LNFFCTCHHN ETKNVGTIYI
KNEDIINFSK AYNKESSILQ YIDVTPYYLK DTYICDFTQN HYSISFDTSV NVQNVLERYL
KILSDLYNTH EEFTYFSIHL KLKKEIMKKK YIDYLKKKIN EYKEKETSDK IKRVTLSTND
NINTILVYRC NIDLGSFDKF KIKCPSKLNE EEVENNKLYP NLIYSSNLGL DETDMLNGLT
KLLYGSVLIN KTEKNVSFFE KGELELIISP YTDSSKNIIF SCENVPRNLS KGIIGSASIF
IKKNDNKILG CDFIDTPSTL SSASTLESSY GSHASSPLSS SHHVLHNDNQ GHDVHMINHI
DISNKKNSFE FEIELIEGKN TYCNIEAIEN DIVGFSCPYN FLTTPSDCFE SIQIEGVDKE
LETHKLEKLL KGVKILNNDI YKYNFTPSYI ILPKKIKKSL KIFCRCNSVK LIKTGIIQIN
IVGDDLNNWF KKEITHNIFA YQKMDYFYDF SKGPTNISSE NVLGISTMSL MSSNKKVSRK
KNHKEENRTQ QNVYKEIEND HKNINENVNK YDNLPVTLLS SDEGDGYQAD EDIGGEDDAE
DVDGEGDDED DNILNPLRTK QVYDIIVAAS EFSKIEVVCP LRNSSQFRQS KISPENFFEY
VYVLEDKNDD KRKRSIEENE KLVKAILEGK KNIDGHIINI EDINNKKSSK NASVEYDDMG
NKIFISIISE KPKAVIGDNI SSSRSSVHIS NNIMNSSFQS NIHPDPITSD TTTSEYEQYN
SYFKDILVIK NINEVISFAN IKIDINEQTY SSSLHIPPLI LKDAEFLISC DNSLTLNENT
RGKTATVKIK VKSNFLKIYG CDFVGEFSTH FLFSKKWDDI PKNYICKINI QDDMLIGLAC
PSFTKLHPPD CFENIIVNQN VYKKNIIMET KNMFFYKQND KPILSFVHVK KILVETFLCK
CYQVTKADYK EVTIQILYEP YVMGTPKYTL EKSIIQYRYA NLKPPLHI
