ID   P230_PLAFO              Reviewed;        3135 AA.
AC   P68875; Q08372;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Gametocyte surface protein P230;
DE   Flags: Precursor;
GN   Name=PF230; Synonyms=S230;
OS   Plasmodium falciparum (isolate NF54).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX   NCBI_TaxID=5843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, DEVELOPMENTAL STAGE,
RP   AND SUBCELLULAR LOCATION.
RX   PubMed=8479460; DOI=10.1016/0166-6851(93)90058-6;
RA   Williamson K.C., Criscio M.D., Kaslow D.C.;
RT   "Cloning and expression of the gene for Plasmodium falciparum transmission-
RT   blocking target antigen, Pfs230.";
RL   Mol. Biochem. Parasitol. 58:355-358(1993).
RN   [2]
RP   SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND BIOTECHNOLOGY.
RX   PubMed=32709983; DOI=10.1038/s42003-020-01123-9;
RA   Singh K., Burkhardt M., Nakuchima S., Herrera R., Muratova O., Gittis A.G.,
RA   Kelnhofer E., Reiter K., Smelkinson M., Veltri D., Swihart B.J.,
RA   Shimp R. Jr., Nguyen V., Zhang B., MacDonald N.J., Duffy P.E.,
RA   Garboczi D.N., Narum D.L.;
RT   "Structure and function of a malaria transmission blocking vaccine
RT   targeting Pfs230 and Pfs230-Pfs48/45 proteins.";
RL   Commun. Biol. 3:395-395(2020).
RN   [3]
RP   SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND BIOTECHNOLOGY.
RX   PubMed=33741942; DOI=10.1038/s41467-021-21955-1;
RA   Coelho C.H., Tang W.K., Burkhardt M., Galson J.D., Muratova O.,
RA   Salinas N.D., Alves E Silva T.L., Reiter K., MacDonald N.J., Nguyen V.,
RA   Herrera R., Shimp R., Narum D.L., Byrne-Steele M., Pan W., Hou X.,
RA   Brown B., Eisenhower M., Han J., Jenkins B.J., Doritchamou J.Y.A.,
RA   Smelkinson M.G., Vega-Rodriguez J., Trueck J., Taylor J.J., Sagara I.,
RA   Renn J.P., Tolia N.H., Duffy P.E.;
RT   "A human monoclonal antibody blocks malaria transmission and defines a
RT   highly conserved neutralizing epitope on gametes.";
RL   Nat. Commun. 12:1750-1750(2021).
CC   -!- FUNCTION: Gametocyte surface protein required for male/female gamete
CC       fusion. Also required for male gamete exflagellation and interaction
CC       with erythrocytes. {ECO:0000250|UniProtKB:P68874}.
CC   -!- SUBUNIT: Heterodimer; heterodimerizes with PF45/48.
CC       {ECO:0000250|UniProtKB:P68874}.
CC   -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000269|PubMed:32709983,
CC       ECO:0000269|PubMed:33741942, ECO:0000269|PubMed:8479460}. Cell membrane
CC       {ECO:0000269|PubMed:32709983, ECO:0000269|PubMed:33741942,
CC       ECO:0000269|PubMed:8479460}; Peripheral membrane protein
CC       {ECO:0000305|PubMed:33741942}; Extracellular side
CC       {ECO:0000269|PubMed:33741942}.
CC   -!- DEVELOPMENTAL STAGE: Specifically expressed in gametocytes, gametes,
CC       and zygotes (at protein level) (PubMed:8479460, PubMed:33741942,
CC       PubMed:32709983). Not expressed in ookinetes (PubMed:33741942).
CC       {ECO:0000269|PubMed:32709983, ECO:0000269|PubMed:33741942,
CC       ECO:0000269|PubMed:8479460}.
CC   -!- PTM: May be processed into a 310 kDa form as the parasite emerges from
CC       the host erythrocytes. {ECO:0000250|UniProtKB:P68874}.
CC   -!- BIOTECHNOLOGY: Promising transmission-blocking vaccine candidate:
CC       targeting the protein would prevents transmission of the parasite
CC       decreasing the malaria burden (PubMed:33741942, PubMed:32709983).
CC       Antibodies against the 6-Cys domain 1 when given together with parasite
CC       gametocytes prevents transmission in the mosquito (PubMed:33741942,
CC       PubMed:32709983). {ECO:0000269|PubMed:32709983,
CC       ECO:0000269|PubMed:33741942}.
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DR   EMBL; L08135; AAA29734.1; -; mRNA.
DR   PIR; A48584; A48584.
DR   SMR; P68875; -.
DR   PRIDE; P68875; -.
DR   VEuPathDB; PlasmoDB:PfNF54_020013800; -.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.2860; -; 9.
DR   InterPro; IPR010884; 6_CYS_dom.
DR   InterPro; IPR038160; 6_CYS_dom_sf.
DR   Pfam; PF07422; s48_45; 7.
DR   SMART; SM00970; s48_45; 9.
DR   PROSITE; PS51701; 6_CYS; 14.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Malaria; Membrane; Repeat; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..3135
FT                   /note="Gametocyte surface protein P230"
FT                   /id="PRO_0000024616"
FT   DOMAIN          589..730
FT                   /note="6-Cys 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          733..887
FT                   /note="6-Cys 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          918..1133
FT                   /note="6-Cys 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          1136..1275
FT                   /note="6-Cys 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          1285..1432
FT                   /note="6-Cys 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          1435..1560
FT                   /note="6-Cys 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          1694..1907
FT                   /note="6-Cys 7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          1910..2035
FT                   /note="6-Cys 8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          2052..2199
FT                   /note="6-Cys 9"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          2204..2374
FT                   /note="6-Cys 10"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          2448..2663
FT                   /note="6-Cys 11"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          2666..2827
FT                   /note="6-Cys 12"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          2831..2979
FT                   /note="6-Cys 13"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DOMAIN          2982..3113
FT                   /note="6-Cys 14"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   REGION          266..470
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2410..2432
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        272..323
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        333..347
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        348..375
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        376..443
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        76
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        111
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        135
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        239
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        585
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        821
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        829
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        889
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        961
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1079
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1089
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1153
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1267
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1300
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1452
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1492
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1508
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1621
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1624
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1753
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1804
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1882
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1920
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1954
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1972
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2178
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2199
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2312
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2351
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2439
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2457
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2466
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2504
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2586
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2611
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2650
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2677
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2688
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2952
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3011
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3016
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3066
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3093
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3096
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        593..611
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        626..706
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        737..781
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        804..862
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1140..1161
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1175..1251
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1200..1249
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1439..1459
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1473..1534
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1483..1532
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1698..1726
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1740..1881
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1914..1938
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1952..2017
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        1963..2015
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2056..2074
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2208..2229
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2243..2356
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2254..2354
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2452..2476
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2490..2638
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2670..2706
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2720..2804
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2730..2802
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        2986..3010
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        3024..3090
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
FT   DISULFID        3035..3088
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01038"
SQ   SEQUENCE   3135 AA;  363218 MW;  BE5F850C87ED9C77 CRC64;
     MKKIITLKNL FLIILVYIFS EKKDLRCNVI KGNNIKDDED KRFHLFYYSH NLFKTPETKE
     KKNKKECFYK NGGIYNLSKE IRMRKDTSVK IKQRTCPFHK EGSSFEMGSK NITCFYPIVG
     KKERKTLDTI IIKKNVTNDH VVSSDMHSNV QEKNMILIRN IDKENKNDIQ NVEEKIQRDT
     YENKDYESDD TLIEWFDDNT NEENFLLTFL KRCLMKIFSS PKRKKTVVQK KHKSNFFINS
     SLKYIYMYLT PSDSFNLVRR NRNLDEEDMS PRDNFVIDDE EEEEEEEEEE EEEEEEEEEE
     EEEEYDDYVY EESGDETEEQ LQEEHQEEVG AESSEESFND EDEDSVEARD GDMIRVDEYY
     EDQDGDTYDS TIKNEDVDEE VGEEVGEEVG EEVGEEVGEE VGEEVGEEVG EEVGEEEGEE
     VGEGVGEEVG EEEGEEVGEE EGEYVDEKER QGEIYPFGDE EEKDEGGESF TYEKSEVDKT
     DLFKFIEGGE GDDVYKVDGS KVLLDDDTIS RVSKKHTARD GEYGEYGEAV EDGENVIKII
     RSVLQSGALP SVGVDELDKI DLSYETTESG DTAVSEDSYD KYASNNTNKE YVCDFTDQLK
     PTESGPKVKK CEVKVNEPLI KVKIICPLKG SVEKLYDNIE YVPKKSPYVV LTKEETKLKE
     KLLSKLIYGL LISPTVNEKE NNFKEGVIEF TLPPVVHKAT VFYFICDNSK TEDDNKKGNR
     GIVEVYVEPY GNKINGCAFL DEDEEEEKYG NQIEEDEHNE KIKMKTFFTQ NIYKKNNIYP
     CYMKLYSGDI GGILFPKNIK STTCFEEMIP YNKEIKWNKE NKSLGNLVNN SVVYNKEMNA
     KYFNVQYVHI PTSYKDTLNL FCSIILKEEE SNLISTSYLV YVSINEELNF SLFDFYESFV
     PIKKTIQVAQ KNVNNKEHDY TCDFTDKLDK TVPSTANGKK LFICRKHLKE FDTFTLKCNV
     NKTQYPNIEI FPKTLKDKKE VLKLDLDIQY QMFSKFFKFN TQNAKYLNLY PYYLIFPFNH
     IGKKELKNNP TYKNHKDVKY FEQSSVLSPL SSADSLGKLL NFLDTQETVC LTEKIRYLNL
     SINELGSDNN TFSVTFQVPP YIDIKEPFYF MFGCNNNKGE GNIGIVELLI SKQEEKIKGC
     NFHESKLDYF NENISSDTHE CTLHAYENDI IGFNCLETTH PNEVEVEVED AEIYLQPENC
     FNNVYKGLNS VDITTILKNA QTYNINNKKT PTFLKIPPYN LLEDVEISCQ CTIKQVVKKI
     KVIITKNDTV LLKREVQSES TLDDKIYKCE HENFINPRVN KTFDENVEYT CNIKIENFFN
     YIQIFCPAKD LGIYKNIQMY YDIVKPTRVP QFKKFNNEEL HKLIPNSEML HKTKEMLILY
     NEEKVDLLHF YVFLPIYIKD IYEFNIVCDN SKTMWKNQLG GKVIYHITVS KREQKVKGCS
     FDNEHAHMFS YNKTNVKNCI IDAKPKDLIG FVCPSGTLKL TNCFKDAIVH TNLTNINGIL
     YLKNNLANFT YKHQFNYMEI PALMDNDISF KCICVDLKKK KYNVKSPLGP KVLRALYKKL
     NIKFDNYVTG TDQNKYLMTY MDLHLSHKRN YLKELFHDLG KKKPADTDAN PESIIESLSI
     NESNESGPFP TGDVDAEHLI LEGYDTWESL YDEQLEEVIY NDIESLELKD IEQYVLQVNL
     KAPKLMMSAQ IHNNRHVCDF SKNNLIVPES LKKKEELGGN PVNIHCYALL KPLDTLYVKC
     PTSKDNYEAA KVNISENDNE YELQVISLIE KRFHNFETLE SKKPGNGDVV VHNGVVDTGP
     VLDNSTFEKY FKNIKIKPDK FFEKVINEYD DTEEEKDLES ILPGAIVSPM KVLKKKDPFT
     SYAAFVVPPI VPKDLHFKVE CNNTEYKDEN QYISGYNGII HIDISNSNRK INGCDFSTNN
     SSILTSSVKL VNGETKNCEI NINNNEVFGI ICDNETNLDP EKCFHEIYSK DNKTVKKFRE
     VIPNIDIFSL HNSNKKKVAY AKVPLDYINK LLFSCSCKTS HTNTIGTMKV TLNKDEKEEE
     DFKTAQGIKH NNVHLCNFFD NPELTFDNNK IVLCKIDAEL FSEVIIQLPI FGTKNVEEGV
     QNEEYKKFSL KPSLVFDDNN NDIKVIGKEK NEVSISLALK GVYGNRIFTF DKNGKKGEGI
     SFFIPPIKQD TDLKFIINET IDNSNIKQRG LIYIFVRKNV SENSFKLCDF TTGSTSLMEL
     NSQVKEKKCT VKIKKGDIFG LKCPKGFAIF PQACFSNVLL EYYKSDYEDS EHINYYIHKD
     KKYNLKPKDV IELMDENFRE LQNIQQYTGI SNITDVLHFK NFNLGNLPLN FKNHYSTAYA
     KVPDTFNSII NFSCNCYNPE KHVYGTMQVE SDNRNFDNIK KNENVIKNFL LPNIEKYALL
     LDDEERQKKI KQQQEEEQQE QILKDQDDRL SRHDDYNKNH TYILYDSNEH ICDYEKNESL
     ISTLPNDTKK IQKSICKINA KALDVVTIKC PHTKNFTPKD YFPNSSLITN DKKIVITFDK
     KNFVTYIDPT KKTFSLKDIY IQSFYGVSLD HLNQIKKIHE EWDDVHLFYP PHNVLHNVVL
     NNHIVNLSSA LEGVLFMKSK VTGDETATKK NTTLPTDGVS SILIPPYVKE DITFHLFCGK
     STTKKPNKKN TSLALIHIHI SSNRNIIHGC DFLYLENQTN DAISNNNNNS YSIFTHNKNT
     ENNLICDISL IPKTVIGIKC PNKKLNPQTC FDEVYYVKQE DVPSKTITAD KYNTFSKDKI
     GNILKNAISI NNPDEKDNTY TYLILPEKFE EELIDTKKVL ACTCDNKYII HMKIEKSTMD
     KIKIDEKKTI GKDICKYDVT TKVATCEIID TIDSSVLKEH HTVHYSITLS RWDKLIIKYP
     TNEKTHFENF FVNPFNLKDK VLYNYNKPIN IEHILPGAIT TDIYDTRTKI KQYILRIPPY
     VHKDIHFSLE FNNSLSLTKQ NQNIIYGNVA KIFIHINQGY KEIHGCDFTG KYSHLFTYSK
     KPLPNDDDIC NVTIGNNTFS GFACLSHFEL KPNNCFSSVY DYNEANKVKK LFDLSTKVEL
     DHIKQNTSGY TLSYIIFNKE STKLKFSCTC SSNYSNYTIR ITFDPNYIIP EPQSRAIIKY
     VDLQDKNFAK YLRKL