P24B3_ARATH
ID P24B3_ARATH Reviewed; 214 AA.
AC Q9LIL4;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Transmembrane emp24 domain-containing protein p24beta3;
DE AltName: Full=p24 family protein beta2;
DE Short=p24beta2;
DE AltName: Full=p24 family protein beta3;
DE Short=p24beta3;
DE Flags: Precursor;
GN OrderedLocusNames=At3g22845; ORFNames=MWI23.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=22577184; DOI=10.1093/jxb/ers112;
RA Montesinos J.C., Sturm S., Langhans M., Hillmer S., Marcote M.J.,
RA Robinson D.G., Aniento F.;
RT "Coupled transport of Arabidopsis p24 proteins at the ER-Golgi interface.";
RL J. Exp. Bot. 63:4243-4261(2012).
RN [6]
RP GENE FAMILY, SUBCELLULAR LOCATION, AND COILED-COIL DOMAIN.
RX PubMed=22132757; DOI=10.1111/j.1600-0854.2011.01317.x;
RA Chen J., Qi X., Zheng H.;
RT "Subclass-specific localization and trafficking of Arabidopsis p24 proteins
RT in the ER-Golgi interface.";
RL Traffic 13:400-415(2012).
CC -!- FUNCTION: Involved in vesicular protein trafficking. Mainly functions
CC in the early secretory pathway but also in post-Golgi membranes.
CC Thought to act as cargo receptor at the lumenal side for incorporation
CC of secretory cargo molecules into transport vesicles and to be involved
CC in vesicle coat formation at the cytoplasmic side (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Probably oligomerizes with other members of the EMP24/GP25L
CC family. Associates with the COPI vesicle coat (coatomer). Associates
CC with the COPII vesicle coat (coatomer). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000269|PubMed:22132757}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:22132757}. Golgi apparatus, Golgi stack membrane
CC {ECO:0000269|PubMed:22132757}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:22132757}. Note=Cycles between the endoplasmic
CC reticulum and Golgi via COPI and COPII dependent pathways.
CC {ECO:0000250}.
CC -!- DOMAIN: The cytoplasmic C-terminal domain contains an Arg-Val motif,
CC which is involved in the anterograde ER-to-Golgi transport of the
CC protein. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
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DR EMBL; AP001300; BAB03029.1; -; Genomic_DNA.
DR EMBL; AB022223; BAB03029.1; JOINED; Genomic_DNA.
DR EMBL; CP002686; AEE76683.1; -; Genomic_DNA.
DR EMBL; AY070742; AAL50082.1; -; mRNA.
DR EMBL; AY093742; AAM10366.1; -; mRNA.
DR RefSeq; NP_188924.3; NM_113184.5.
DR AlphaFoldDB; Q9LIL4; -.
DR SMR; Q9LIL4; -.
DR BioGRID; 7188; 3.
DR IntAct; Q9LIL4; 3.
DR STRING; 3702.AT3G22845.1; -.
DR SwissPalm; Q9LIL4; -.
DR PaxDb; Q9LIL4; -.
DR PRIDE; Q9LIL4; -.
DR ProteomicsDB; 248859; -.
DR EnsemblPlants; AT3G22845.1; AT3G22845.1; AT3G22845.
DR GeneID; 821856; -.
DR Gramene; AT3G22845.1; AT3G22845.1; AT3G22845.
DR KEGG; ath:AT3G22845; -.
DR Araport; AT3G22845; -.
DR TAIR; locus:2094364; AT3G22845.
DR eggNOG; KOG1692; Eukaryota.
DR HOGENOM; CLU_066963_1_1_1; -.
DR InParanoid; Q9LIL4; -.
DR OMA; NAEDCFY; -.
DR OrthoDB; 1328081at2759; -.
DR PhylomeDB; Q9LIL4; -.
DR PRO; PR:Q9LIL4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LIL4; baseline and differential.
DR Genevisible; Q9LIL4; AT.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR InterPro; IPR015720; Emp24-like.
DR InterPro; IPR009038; GOLD_dom.
DR InterPro; IPR036598; GOLD_dom_sf.
DR PANTHER; PTHR22811; PTHR22811; 1.
DR Pfam; PF01105; EMP24_GP25L; 1.
DR SMART; SM01190; EMP24_GP25L; 1.
DR SUPFAM; SSF101576; SSF101576; 1.
DR PROSITE; PS50866; GOLD; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; ER-Golgi transport; Glycoprotein; Golgi apparatus; Membrane;
KW Methylation; Protein transport; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..214
FT /note="Transmembrane emp24 domain-containing protein
FT p24beta3"
FT /id="PRO_0000419793"
FT TOPO_DOM 28..178
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 35..122
FT /note="GOLD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
FT COILED 140..158
FT /evidence="ECO:0000255"
FT MOTIF 204..214
FT /note="COPI vesicle coat-binding"
FT /evidence="ECO:0000255"
FT MOTIF 204..205
FT /note="COPII vesicle coat-binding"
FT /evidence="ECO:0000255"
FT MOTIF 213..214
FT /note="Required for the export from the endoplasmic
FT reticulum to the Golgi"
FT /evidence="ECO:0000250"
FT MOD_RES 164
FT /note="Omega-N-methylated arginine"
FT /evidence="ECO:0000250"
FT MOD_RES 169
FT /note="Omega-N-methylated arginine"
FT /evidence="ECO:0000250"
FT CARBOHYD 172
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 214 AA; 24282 MW; 5C0C31625AF7A134 CRC64;
MERRQAKIHV FVLIGLILLN SINQISSLSV TVNDEECVQE YVLYEGDTVS GNFVVVDHDI
FWGSDHPGLD FTVTSPAGNI VQTLKGTSGD KFEFKAPKSG MYKFCFHNPY STPETVSFYI
HVGHIPNEHD LAKDEHLDPV NVKIAELREA LESVVAEQKY LKARDTRHRH TNESTRKRVI
FYTVGEYIFL AAASGLQVLY IRKLFSKSVA YNRV
