P25_SCHPO
ID P25_SCHPO Reviewed; 202 AA.
AC P30821;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=P25 protein;
DE AltName: Full=Brefeldin A resistance protein;
GN Name=obr1; ORFNames=SPAC3C7.14c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 36-56.
RX PubMed=1448080; DOI=10.1128/mcb.12.12.5474-5484.1992;
RA Toda T., Shimanuki M., Saka Y., Yamano H., Adachi Y., Shirakawa M.,
RA Kyogoku Y., Yanagida M.;
RT "Fission yeast pap1-dependent transcription is negatively regulated by an
RT essential nuclear protein, crm1.";
RL Mol. Cell. Biol. 12:5474-5484(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7929079; DOI=10.1016/s0021-9258(19)51072-9;
RA Turi T.G., Webster P., Rose J.K.;
RT "Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe.
RT Isolation of multiple genes conferring resistance.";
RL J. Biol. Chem. 269:24229-24236(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Unknown. Target of pap1 transcription factor. Confers
CC brefeldin A resistance in S.pombe.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the WrbA family. {ECO:0000305}.
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DR EMBL; D13038; BAA02370.1; -; Genomic_DNA.
DR EMBL; X73558; CAA51956.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB16744.1; -; Genomic_DNA.
DR PIR; A45029; A45029.
DR RefSeq; NP_593615.1; NM_001019046.2.
DR AlphaFoldDB; P30821; -.
DR SMR; P30821; -.
DR BioGRID; 280100; 23.
DR IntAct; P30821; 2.
DR MINT; P30821; -.
DR STRING; 4896.SPAC3C7.14c.1; -.
DR iPTMnet; P30821; -.
DR MaxQB; P30821; -.
DR PaxDb; P30821; -.
DR PRIDE; P30821; -.
DR EnsemblFungi; SPAC3C7.14c.1; SPAC3C7.14c.1:pep; SPAC3C7.14c.
DR GeneID; 2543686; -.
DR KEGG; spo:SPAC3C7.14c; -.
DR PomBase; SPAC3C7.14c; obr1.
DR VEuPathDB; FungiDB:SPAC3C7.14c; -.
DR eggNOG; KOG3135; Eukaryota.
DR HOGENOM; CLU_051402_0_1_1; -.
DR InParanoid; P30821; -.
DR OMA; GYSHTFP; -.
DR PhylomeDB; P30821; -.
DR PRO; PR:P30821; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0031934; C:mating-type region heterochromatin; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; ISM:PomBase.
DR Gene3D; 3.40.50.360; -; 1.
DR InterPro; IPR008254; Flavodoxin/NO_synth.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR010089; Flavoprotein_WrbA-like.
DR InterPro; IPR005025; FMN_Rdtase-like.
DR Pfam; PF03358; FMN_red; 1.
DR SUPFAM; SSF52218; SSF52218; 1.
DR TIGRFAMs; TIGR01755; flav_wrbA; 1.
DR PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Phosphoprotein; Reference proteome.
FT CHAIN 1..202
FT /note="P25 protein"
FT /id="PRO_0000200766"
FT DOMAIN 7..195
FT /note="Flavodoxin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
FT MOD_RES 181
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 202 AA; 21899 MW; 54E1DB3A8F7E9DB2 CRC64;
MSTANTVAIV IYSTYGHVVK LAEAEKAGIE KAGGKAVIYQ FPETLSPEIL EKMHAAPKPN
YPVVTLDVLT QYDAFLFGYP TRYGTPPAQF RTFWDSTGGL WVQGALHGKY FGQFFSTGTL
GGGQESTALT AMTSFVHHGM IFVPLGYKNT FSLMANVESI HGGSSWGAGS YAGADGSRNV
SDDELEIARI QGETFFKTVF RK
