ID   P28_MONPZ               Reviewed;         242 AA.
AC   Q8V571;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   23-FEB-2022, entry version 82.
DE   RecName: Full=E3 ubiquitin-protein ligase p28-like;
DE            EC=2.3.2.27;
DE   AltName: Full=Protein D5R;
DE   AltName: Full=RING-type E3 ubiquitin transferase p28-like {ECO:0000305};
GN   Name=p28; ORFNames=D5R;
OS   Monkeypox virus (strain Zaire-96-I-16) (MPX).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=619591;
OH   NCBI_TaxID=45479; Cynomys gunnisoni (Gunnison's prairie dog).
OH   NCBI_TaxID=99825; Cynomys leucurus (White-tailed prairie dog).
OH   NCBI_TaxID=45480; Cynomys ludovicianus (Black-tailed prairie dog).
OH   NCBI_TaxID=99826; Cynomys mexicanus (Mexican prairie dog).
OH   NCBI_TaxID=99827; Cynomys parvidens (Utah prairie dog).
OH   NCBI_TaxID=30650; Gliridae (dormice).
OH   NCBI_TaxID=226685; Heliosciurus ruwenzorii (Ruwenzori sun squirrel).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Zaire-96-I-16;
RX   PubMed=11734207; DOI=10.1016/s0014-5793(01)03144-1;
RA   Shchelkunov S.N., Totmenin A.V., Babkin I.V., Safronov P.F.,
RA   Ryazankina O.I., Petrov N.A., Gutorov V.V., Uvarova E.A., Mikheev M.V.,
RA   Sisler J.R., Esposito J.J., Jahrling P.B., Moss B., Sandakhchiev L.S.;
RT   "Human monkeypox and smallpox viruses: genomic comparison.";
RL   FEBS Lett. 509:66-70(2001).
CC   -!- FUNCTION: RING-finger E3 ubiquitin ligase which catalyzes the formation
CC       of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Plays an
CC       important role in virulence by acting as an anti-apoptotic factor.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm. Note=Localizes to viral
CC       factories, the sites of virus replication. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae p28 protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF380138; AAL40466.1; -; Genomic_DNA.
DR   RefSeq; NP_536435.1; NC_003310.1.
DR   GeneID; 928906; -.
DR   KEGG; vg:928906; -.
DR   Proteomes; UP000101269; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0039648; P:modulation by virus of host protein ubiquitination; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR016398; E3_ubiquitin-prot_ligase_p28.
DR   InterPro; IPR017880; KilA_N.
DR   InterPro; IPR018004; KilA_N/APSES_HTH.
DR   InterPro; IPR045072; MKRN-like.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   InterPro; IPR017907; Znf_RING_CS.
DR   PANTHER; PTHR11224; PTHR11224; 1.
DR   Pfam; PF04383; KilA-N; 1.
DR   Pfam; PF13639; zf-RING_2; 1.
DR   PIRSF; PIRSF003775; E3_ubiquit_lig_p28; 1.
DR   SMART; SM00184; RING; 1.
DR   PROSITE; PS51301; KILA_N; 1.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; Metal-binding;
KW   Modulation of host cell apoptosis by virus;
KW   Modulation of host ubiquitin pathway by viral E3 ligase;
KW   Modulation of host ubiquitin pathway by virus; Transferase;
KW   Ubl conjugation pathway; Zinc; Zinc-finger.
FT   CHAIN           1..242
FT                   /note="E3 ubiquitin-protein ligase p28-like"
FT                   /id="PRO_0000395988"
FT   DOMAIN          21..131
FT                   /note="KilA-N"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00631"
FT   ZN_FING         173..226
FT                   /note="RING-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
SQ   SEQUENCE   242 AA;  28640 MW;  F5203CE384205BDC CRC64;
     MEFDPVKINT SSIDHVTILQ YIDEPNDIRL TVCIIRNINN ITYYINITKI NPHLANRFRA
     WKKRIAGRDY MTNLSRDTGI QQSKLTETIR NCQKNKNIYG LYIHYNLVIN VVIDWITDVI
     VQSILRGLVN WYIANNTYTP NTPNNTTTIS ELDIIKILDK YEDMYRVSKE KECGICYEVV
     YSKRLENDRY FGLLDSCNHI FCITCINIWH RTRRETGALD NCPICRTRFR KITMSKFYKL
     VN