P2A05_ARATH
ID P2A05_ARATH Reviewed; 411 AA.
AC Q9C5Q9; O80811; Q84JG0; Q84K23; Q84VK8;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein PHLOEM PROTEIN 2-LIKE A5;
DE Short=AtPP2-A5;
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
GN Name=PP2A5; OrderedLocusNames=At1g65390; ORFNames=T8F5.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 15-198, AND VARIANTS THR-42; ARG-89;
RP GLN-97; VAL-108; ILE-116; 123-HIS--LYS-125; ASN-130; LEU-133; HIS-140;
RP GLN-147; MET-150; ASN-167; ARG-188; 191-SER--GLU-193 AND 197-VAL-ASN-198.
RC STRAIN=cv. An-2, cv. Bla-1, cv. Bs-1, cv. Bu-0, cv. Chi-1, cv. Co-1,
RC cv. Cvi-0, cv. El-0, cv. Fi-0, cv. Gr-3, cv. Ita-0, cv. Jl-3, cv. Kas-1,
RC cv. La-0, cv. Landsberg erecta, cv. Lisse-2, cv. Lu-1, cv. Pi-0, and
RC cv. Sf-1;
RX PubMed=12663546; DOI=10.1093/genetics/163.3.1083;
RA Shepard K.A., Purugganan M.D.;
RT "Molecular population genetics of the Arabidopsis CLAVATA2 region: the
RT genomic scale of variation and selection in a selfing species.";
RL Genetics 163:1083-1095(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12529520; DOI=10.1104/pp.013086;
RA Dinant S., Clark A.M., Zhu Y., Vilaine F., Palauqui J.-C., Kusiak C.,
RA Thompson G.A.;
RT "Diversity of the superfamily of phloem lectins (phloem protein 2) in
RT angiosperms.";
RL Plant Physiol. 131:114-128(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9C5Q9-1; Sequence=Displayed;
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC27148.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004512; AAC27148.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34368.1; -; Genomic_DNA.
DR EMBL; AF325110; AAK17178.1; -; mRNA.
DR EMBL; AK176581; BAD44344.1; -; mRNA.
DR EMBL; AF528686; AAO43284.1; -; Genomic_DNA.
DR EMBL; AF528687; AAO43285.1; -; Genomic_DNA.
DR EMBL; AF528688; AAO43286.1; -; Genomic_DNA.
DR EMBL; AF528689; AAO43287.1; -; Genomic_DNA.
DR EMBL; AF528690; AAO43288.1; -; Genomic_DNA.
DR EMBL; AF528691; AAO43289.1; -; Genomic_DNA.
DR EMBL; AF528692; AAO43290.1; -; Genomic_DNA.
DR EMBL; AF528693; AAO43291.1; -; Genomic_DNA.
DR EMBL; AF528694; AAO43292.1; -; Genomic_DNA.
DR EMBL; AF528695; AAO43293.1; -; Genomic_DNA.
DR EMBL; AF528696; AAO43294.1; -; Genomic_DNA.
DR EMBL; AF528697; AAO43295.1; -; Genomic_DNA.
DR EMBL; AF528698; AAO43296.1; -; Genomic_DNA.
DR EMBL; AF528699; AAO43297.1; -; Genomic_DNA.
DR EMBL; AF528700; AAO43298.1; -; Genomic_DNA.
DR EMBL; AF528701; AAO43299.1; -; Genomic_DNA.
DR EMBL; AF528702; AAO43300.1; -; Genomic_DNA.
DR EMBL; AF528703; AAO43301.1; -; Genomic_DNA.
DR EMBL; AF528704; AAO43302.1; -; Genomic_DNA.
DR PIR; T02363; T02363.
DR RefSeq; NP_176718.2; NM_105213.4. [Q9C5Q9-1]
DR AlphaFoldDB; Q9C5Q9; -.
DR SMR; Q9C5Q9; -.
DR BioGRID; 28072; 6.
DR STRING; 3702.AT1G65390.1; -.
DR PaxDb; Q9C5Q9; -.
DR PRIDE; Q9C5Q9; -.
DR DNASU; 842851; -.
DR EnsemblPlants; AT1G65390.1; AT1G65390.1; AT1G65390. [Q9C5Q9-1]
DR GeneID; 842851; -.
DR Gramene; AT1G65390.1; AT1G65390.1; AT1G65390. [Q9C5Q9-1]
DR KEGG; ath:AT1G65390; -.
DR Araport; AT1G65390; -.
DR TAIR; locus:2206255; AT1G65390.
DR eggNOG; KOG0017; Eukaryota.
DR HOGENOM; CLU_786077_0_0_1; -.
DR InParanoid; Q9C5Q9; -.
DR OrthoDB; 1051457at2759; -.
DR PhylomeDB; Q9C5Q9; -.
DR PRO; PR:Q9C5Q9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C5Q9; baseline and differential.
DR Genevisible; Q9C5Q9; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0012505; C:endomembrane system; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0030246; F:carbohydrate binding; ISS:TAIR.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0002213; P:defense response to insect; IDA:TAIR.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IEP:TAIR.
DR GO; GO:0032260; P:response to jasmonic acid stimulus involved in jasmonic acid and ethylene-dependent systemic resistance; IEP:TAIR.
DR GO; GO:0009863; P:salicylic acid mediated signaling pathway; IEP:TAIR.
DR Gene3D; 3.40.50.10140; -; 1.
DR InterPro; IPR025886; PP2-like.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR Pfam; PF14299; PP2; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR PROSITE; PS50104; TIR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; NAD; Reference proteome.
FT CHAIN 1..411
FT /note="Protein PHLOEM PROTEIN 2-LIKE A5"
FT /id="PRO_0000285280"
FT DOMAIN 20..157
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT ACT_SITE 94
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT VARIANT 42
FT /note="K -> T (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 89
FT /note="H -> R (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 97
FT /note="K -> Q (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 108
FT /note="I -> V (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 116
FT /note="L -> I (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 123..125
FT /note="DLT -> HLK (in strain: cv. An-2, cv. Bs-1, cv. Bu-0,
FT cv. Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv.
FT Ita-0, cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv.
FT Lisse-2, cv. Lu-1, cv. Pi-0 and cv. Sf-1)"
FT VARIANT 130
FT /note="D -> N (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 133
FT /note="W -> L (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 140
FT /note="Q -> H (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 147
FT /note="H -> Q (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 150
FT /note="T -> M (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 167
FT /note="H -> N (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Kas-1, cv. Landsberg erecta, cv. Lisse-2, cv.
FT Lu-1, cv. Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 188
FT /note="K -> R (in strain: cv. An-2, cv. Bs-1, cv. Bu-0, cv.
FT Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv. Ita-0,
FT cv. Jl-3, cv. Landsberg erecta, cv. Lisse-2, cv. Lu-1, cv.
FT Pi-0 and cv. Sf-1)"
FT /evidence="ECO:0000269|PubMed:12663546"
FT VARIANT 191..193
FT /note="FQR -> SQE (in strain: cv. An-2, cv. Bs-1, cv. Bu-0,
FT cv. Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv.
FT Ita-0, cv. Jl-3, cv. Landsberg erecta, cv. Lisse-2, cv. Lu-
FT 1, cv. Pi-0 and cv. Sf-1)"
FT VARIANT 197..198
FT /note="EI -> VN (in strain: cv. An-2, cv. Bs-1, cv. Bu-0,
FT cv. Chi-1, cv. Co-1, cv. Cvi-0, cv. El-0, cv. Gr-3, cv.
FT Ita-0, cv. Jl-3, cv. Landsberg erecta, cv. Lisse-2, cv. Lu-
FT 1, cv. Pi-0 and cv. Sf-1)"
SQ SEQUENCE 411 AA; 47963 MW; EFF793E15C1B4A79 CRC64;
MSGASSVSSI CSSNVSLIPT GPQVFINFRG KDLRKGFMSF LKPALKKEKI NVFIDEQEER
GKYLISLFDT IGESKIALVI FSEGYCESHW CMDELVKIKE YMDQNRLIII PIFYRLDLDV
VKDLTGKFGD NFWDLVDKYQ PEPKKLHKWT EALFSVCELF SLILPKHSDI SDRDFVKSIV
KAVKKVQKNF FQRRNGEIEY QDFSVPACKL TITMHESPNE EAVQVTVLNE FYQMKNQSPV
PSYEFKFWVD LTRPKGNVFM IDARDLSIAW SEDSNHWTWL PLPNQNSNES VMEIAFLKSA
SWLDVAGKFD TRYLTPRTRY EVVFVVKLEY TFEWETLVKL KLDLPNTWEK PQEQSVDMFD
YISDQWLDIP VGEFTTSKKN VGEISFAMYE HECQLWKSGL FVKGVTIRPK Y
