ID   P2A08_ARATH             Reviewed;         354 AA.
AC   Q9FHE9;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Protein PHLOEM PROTEIN 2-LIKE A8;
DE            Short=AtPP2-A8;
DE            EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
GN   Name=PP2A8; OrderedLocusNames=At5g45070; ORFNames=K17O22.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis cDNA clones.";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12529520; DOI=10.1104/pp.013086;
RA   Dinant S., Clark A.M., Zhu Y., Vilaine F., Palauqui J.-C., Kusiak C.,
RA   Thompson G.A.;
RT   "Diversity of the superfamily of phloem lectins (phloem protein 2) in
RT   angiosperms.";
RL   Plant Physiol. 131:114-128(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC         Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC   -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC       Self-association of TIR domains is required for NADase activity.
CC       {ECO:0000255|PROSITE-ProRule:PRU00204}.
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DR   EMBL; AB019224; BAB09490.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95197.1; -; Genomic_DNA.
DR   EMBL; BT010864; AAR24231.1; -; mRNA.
DR   EMBL; BT012618; AAT06437.1; -; mRNA.
DR   RefSeq; NP_199320.1; NM_123875.3.
DR   AlphaFoldDB; Q9FHE9; -.
DR   SMR; Q9FHE9; -.
DR   STRING; 3702.AT5G45070.1; -.
DR   iPTMnet; Q9FHE9; -.
DR   PaxDb; Q9FHE9; -.
DR   PRIDE; Q9FHE9; -.
DR   ProteomicsDB; 248688; -.
DR   EnsemblPlants; AT5G45070.1; AT5G45070.1; AT5G45070.
DR   GeneID; 834538; -.
DR   Gramene; AT5G45070.1; AT5G45070.1; AT5G45070.
DR   KEGG; ath:AT5G45070; -.
DR   Araport; AT5G45070; -.
DR   TAIR; locus:2153212; AT5G45070.
DR   eggNOG; KOG0017; Eukaryota.
DR   HOGENOM; CLU_786077_0_0_1; -.
DR   InParanoid; Q9FHE9; -.
DR   OMA; NEREFMN; -.
DR   OrthoDB; 898150at2759; -.
DR   PhylomeDB; Q9FHE9; -.
DR   PRO; PR:Q9FHE9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Genevisible; Q9FHE9; AT.
DR   GO; GO:0030246; F:carbohydrate binding; ISS:TAIR.
DR   GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   Gene3D; 3.40.50.10140; -; 1.
DR   InterPro; IPR025886; PP2-like.
DR   InterPro; IPR000157; TIR_dom.
DR   InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR   Pfam; PF14299; PP2; 1.
DR   Pfam; PF01582; TIR; 1.
DR   SMART; SM00255; TIR; 1.
DR   SUPFAM; SSF52200; SSF52200; 1.
DR   PROSITE; PS50104; TIR; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; NAD; Reference proteome.
FT   CHAIN           1..354
FT                   /note="Protein PHLOEM PROTEIN 2-LIKE A8"
FT                   /id="PRO_0000285283"
FT   DOMAIN          12..179
FT                   /note="TIR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT   ACT_SITE        86
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
SQ   SEQUENCE   354 AA;  40106 MW;  2E2A1E525B377997 CRC64;
     MAASSSVRPT PTGPQVFINF RGKDLRNGFL SFLEPAMREA NINVFIDKDE VVGTDLVNLF
     VRIQESRVAV VIFSKDYTSS EWCLDELAEI KDCINQGGLN AIPIFYKLAP SSVLELKGGF
     GDTFRVLKEK YKNDPERTQK WQEALESIPK LKGLRLAEKS DRNEREFMNE MILEIQKALW
     QIAMKGNPKV ESNSKGGFLV PARRLTIAHS DNPEKWTWSA IYDRPHKADI EIATMINTHA
     LIKISGDFHT RKLIPGKKYE VVFIVSLDDT SLGWKNEVTL TLKVVMSDEA ANVKAKKLCL
     DEYIGENWVD IPVGDFEAPQ EKEDAKIFFS MYQLLNTERK SGLVVKGFAI RPAQ