P2C01_ORYSJ
ID P2C01_ORYSJ Reviewed; 331 AA.
AC Q942P9; A0A0P0UYM8;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Probable protein phosphatase 2C 1;
DE Short=OsPP2C01;
DE EC=3.1.3.16;
GN OrderedLocusNames=Os01g0164600, LOC_Os01g07090;
GN ORFNames=B1189A09.48, OsJ_000491, P0701D05.3;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT Arabidopsis.";
RL BMC Genomics 9:550-550(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK099645; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AP003209; BAC00581.1; -; Genomic_DNA.
DR EMBL; AP003301; BAB64790.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04017.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS70557.1; -; Genomic_DNA.
DR EMBL; CM000138; EAZ10666.1; -; Genomic_DNA.
DR EMBL; AK099645; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015626680.1; XM_015771194.1.
DR PDB; 6AE9; X-ray; 1.47 A; A/B=44-294.
DR PDBsum; 6AE9; -.
DR AlphaFoldDB; Q942P9; -.
DR SMR; Q942P9; -.
DR STRING; 4530.OS01T0164600-01; -.
DR PaxDb; Q942P9; -.
DR PRIDE; Q942P9; -.
DR EnsemblPlants; Os01t0164600-01; Os01t0164600-01; Os01g0164600.
DR GeneID; 4324414; -.
DR Gramene; Os01t0164600-01; Os01t0164600-01; Os01g0164600.
DR KEGG; osa:4324414; -.
DR eggNOG; KOG1379; Eukaryota.
DR HOGENOM; CLU_029404_3_3_1; -.
DR InParanoid; Q942P9; -.
DR OMA; MQSIYWT; -.
DR OrthoDB; 826926at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q942P9; OS.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.60.40.10; -; 1.
DR InterPro; IPR036457; PPM-type_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase_dom.
DR InterPro; IPR039123; PPTC7.
DR PANTHER; PTHR12320; PTHR12320; 1.
DR Pfam; PF07228; SpoIIE; 1.
DR SMART; SM00331; PP2C_SIG; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; SSF81606; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Manganese; Metal-binding;
KW Protein phosphatase; Reference proteome.
FT CHAIN 1..331
FT /note="Probable protein phosphatase 2C 1"
FT /id="PRO_0000363247"
FT DOMAIN 48..292
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..331
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 315..331
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 79
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 79
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 80
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 210
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 283
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT STRAND 44..51
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 54..59
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 62..68
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 70..72
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 74..80
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 84..87
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 93..105
FT /evidence="ECO:0007829|PDB:6AE9"
FT TURN 109..113
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 115..125
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 131..139
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 143..152
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 154..159
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 171..173
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 180..183
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 189..191
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 193..198
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 204..208
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 210..213
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 218..226
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 231..246
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 254..261
FT /evidence="ECO:0007829|PDB:6AE9"
FT TURN 262..265
FT /evidence="ECO:0007829|PDB:6AE9"
FT HELIX 267..273
FT /evidence="ECO:0007829|PDB:6AE9"
FT STRAND 285..292
FT /evidence="ECO:0007829|PDB:6AE9"
SQ SEQUENCE 331 AA; 35426 MW; BB7431DA8BEA6432 CRC64;
MAASSTATRL SPPRLHAPTT PSPHLPLRRS RFSPLRAAKL EAVLTIGTHL IPHPRKAETG
GEDAFFVNGD DGGVFAVADG VSGWAEKDVN PALFSRELMA HTSTFLKDEE VNHDPQLLLM
KAHAATTSVG SATVIIAMLE KTGILKIASV GDCGLKVIRK GQVMFSTCPQ EHYFDCPYQL
SSEAIGQTYL DALVCTVNLM EGDMIVSGSD GFFDNIFDQE IVSVISESPG VDEAAKALAE
LARKHSVDVT FDSPYSMEAR SRGFDVPSWK KFIGGKLIGG KMDDITVIVA QVKAVMIPDD
EGVDEEKGQG DEQGSAVAVA SSEQKEDSIT T
