P2C14_ORYSJ
ID P2C14_ORYSJ Reviewed; 518 AA.
AC Q6K6N7; A0A0P0VIU2; Q8RVS2;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Probable protein phosphatase 2C 14;
DE Short=OsPP2C14;
DE EC=3.1.3.16;
GN OrderedLocusNames=Os02g0471500, LOC_Os02g27220;
GN ORFNames=P0046H03.1, P0403C01.30;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 389-518.
RC STRAIN=cv. Tainung 57; TISSUE=Seedling leaf;
RA Lee R.H., Chen S.C.G.;
RT "Leaf-associated cDNA encoding a putative protein phosphatase 2C.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT Arabidopsis.";
RL BMC Genomics 9:550-550(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
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DR EMBL; AP004787; BAD19485.1; -; Genomic_DNA.
DR EMBL; AP005065; BAD19608.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08749.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS78624.1; -; Genomic_DNA.
DR EMBL; AK120615; BAH00094.1; -; mRNA.
DR EMBL; AF358769; AAL83985.1; -; mRNA.
DR RefSeq; XP_015626465.1; XM_015770979.1.
DR AlphaFoldDB; Q6K6N7; -.
DR SMR; Q6K6N7; -.
DR STRING; 4530.OS02T0471500-01; -.
DR PaxDb; Q6K6N7; -.
DR PRIDE; Q6K6N7; -.
DR EnsemblPlants; Os02t0471500-01; Os02t0471500-01; Os02g0471500.
DR EnsemblPlants; Os02t0471500-02; Os02t0471500-02; Os02g0471500.
DR GeneID; 4329347; -.
DR Gramene; Os02t0471500-01; Os02t0471500-01; Os02g0471500.
DR Gramene; Os02t0471500-02; Os02t0471500-02; Os02g0471500.
DR KEGG; osa:4329347; -.
DR eggNOG; KOG0698; Eukaryota.
DR HOGENOM; CLU_013173_6_0_1; -.
DR InParanoid; Q6K6N7; -.
DR OMA; AFHVMDK; -.
DR OrthoDB; 1344250at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; Q6K6N7; baseline and differential.
DR Genevisible; Q6K6N7; OS.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR GO; GO:0035970; P:peptidyl-threonine dephosphorylation; IBA:GO_Central.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 3.60.40.10; -; 1.
DR InterPro; IPR036457; PPM-type_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase_dom.
DR Pfam; PF00481; PP2C; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; SSF81606; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW Reference proteome.
FT CHAIN 1..518
FT /note="Probable protein phosphatase 2C 14"
FT /id="PRO_0000363260"
FT DOMAIN 129..437
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 86..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 192..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 193..212
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 165
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 165
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 166
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 382
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 428
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 518 AA; 55284 MW; D9557DD21A873599 CRC64;
MVEAAAGRRS GANRRRPSGG GERRRQQQQH QRLVAVAVAA RVVMVAPAAT PAPAAGGGGG
CVEDILGCLL GVLRALGVTW AAAARPQRQQ PRLAAQTPRG PAPGADGRRA AAELRGIPGR
IAGNGACAVA SLYTLQGKKG VNQDAMIVWE NFCSREDTIF CGVFDGHGPN GHLVAKRVRD
LLPIKLGADL GTDEGRQTST SSIKSNGDET GSPGNMGRDA EQNGEYPEIF TALRTSFLRA
FNVMDRDLKL HKSIDCFFSG TTAVAVLKQG RNLIIGNLGD SRAILGTRDK DNQLMAVQLT
VDLKPNIPSE AQRIRQRRGR IFALPEEPEV ARVWLPKYNS PGLAMARAFG DFCLKDYGLI
SMPEVSYHRI TEKDEFVVLA TDGVWDVLSN TEVVSIVNRA TSRASAARLL VESAHRAWRA
RFPTSKIDDC AVVCLFLDTD ELSETSSSMA RDMTNAVEVS SGQHSNTIQL STGVSSDVVT
AVLTDGDDLS AVDAVAKLVT LTDLPNNASG ATQSITTK
