P2C17_ARATH
ID P2C17_ARATH Reviewed; 283 AA.
AC Q8L7I4; Q94AE3; Q9C9R2;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Probable protein phosphatase 2C 17;
DE Short=AtPP2C17;
DE EC=3.1.3.16;
GN OrderedLocusNames=At1g78200; ORFNames=T11I11.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT Arabidopsis.";
RL BMC Genomics 9:550-550(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52101.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012680; AAG52101.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36080.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36081.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60992.1; -; Genomic_DNA.
DR EMBL; AY048244; AAK82506.1; -; mRNA.
DR EMBL; AY133563; AAM91393.1; -; mRNA.
DR EMBL; AK317294; BAH19970.1; -; mRNA.
DR PIR; D96811; D96811.
DR RefSeq; NP_001323236.1; NM_001334823.1.
DR RefSeq; NP_001323237.1; NM_001334822.1.
DR RefSeq; NP_001323238.1; NM_001334821.1.
DR RefSeq; NP_565172.1; NM_106470.3.
DR RefSeq; NP_974168.1; NM_202439.3.
DR AlphaFoldDB; Q8L7I4; -.
DR SMR; Q8L7I4; -.
DR STRING; 3702.AT1G78200.2; -.
DR iPTMnet; Q8L7I4; -.
DR PaxDb; Q8L7I4; -.
DR PRIDE; Q8L7I4; -.
DR ProteomicsDB; 248873; -.
DR EnsemblPlants; AT1G78200.1; AT1G78200.1; AT1G78200.
DR EnsemblPlants; AT1G78200.2; AT1G78200.2; AT1G78200.
DR EnsemblPlants; AT1G78200.3; AT1G78200.3; AT1G78200.
DR GeneID; 844156; -.
DR Gramene; AT1G78200.1; AT1G78200.1; AT1G78200.
DR Gramene; AT1G78200.2; AT1G78200.2; AT1G78200.
DR Gramene; AT1G78200.3; AT1G78200.3; AT1G78200.
DR KEGG; ath:AT1G78200; -.
DR Araport; AT1G78200; -.
DR TAIR; locus:2194734; AT1G78200.
DR eggNOG; KOG0698; Eukaryota.
DR HOGENOM; CLU_013173_0_1_1; -.
DR InParanoid; Q8L7I4; -.
DR OMA; RRIYMED; -.
DR OrthoDB; 1044139at2759; -.
DR PhylomeDB; Q8L7I4; -.
DR PRO; PR:Q8L7I4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L7I4; baseline and differential.
DR Genevisible; Q8L7I4; AT.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0006470; P:protein dephosphorylation; IBA:GO_Central.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 3.60.40.10; -; 1.
DR InterPro; IPR015655; PP2C.
DR InterPro; IPR036457; PPM-type_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase_dom.
DR PANTHER; PTHR13832; PTHR13832; 1.
DR Pfam; PF00481; PP2C; 1.
DR SMART; SM00331; PP2C_SIG; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; SSF81606; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW Reference proteome.
FT CHAIN 1..283
FT /note="Probable protein phosphatase 2C 17"
FT /id="PRO_0000367947"
FT DOMAIN 32..282
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT BINDING 69
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 69
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 70
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 234
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 273
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT CONFLICT 198..200
FT /note="VSR -> ISS (in Ref. 3; AAK82506)"
FT /evidence="ECO:0000305"
FT CONFLICT 229..231
FT /note="LIL -> IIM (in Ref. 3; AAK82506)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 283 AA; 30854 MW; 576000E070123F3E CRC64;
MPKICCSRSA TQVVVAQKSN SGKGRNGEGG IKYGFSLIKG KSNHSMEDYH VAKFTNFNGN
ELGLFAIFDG HKGDHVAAYL QKHLFSNILK DGEFLVDPRR AIAKAYENTD QKILADNRTD
LESGGSTAVT AILINGKALW IANVGDSRAI VSSRGKAKQM SVDHDPDDDT ERSMIESKGG
FVTNRPGDVP RVNGLLAVSR VFGDKNLKAY LNSEPEIKDV TIDSHTDFLI LASDGISKVM
SNQEAVDVAK KLKDPKEAAR QVVAEALKRN SKDDISCIVV RFR
