P2C24_ARATH
ID P2C24_ARATH Reviewed; 362 AA.
AC Q9ZW21;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Probable protein phosphatase 2C 24;
DE Short=AtPP2C24;
DE EC=3.1.3.16;
GN OrderedLocusNames=At2g29380; ORFNames=F16P2.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT Arabidopsis.";
RL BMC Genomics 9:550-550(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC -!- INTERACTION:
CC Q9ZW21; Q9S7Z2: AFP4; NbExp=4; IntAct=EBI-4441103, EBI-1778843;
CC Q9ZW21; A0SVK0: DOG1; NbExp=5; IntAct=EBI-4441103, EBI-25512274;
CC Q9ZW21; O23160: MYB73; NbExp=3; IntAct=EBI-4441103, EBI-25506855;
CC Q9ZW21; Q8H1R0: PYL10; NbExp=7; IntAct=EBI-4441103, EBI-2363213;
CC Q9ZW21; Q9FJ50: PYL11; NbExp=7; IntAct=EBI-4441103, EBI-2363233;
CC Q9ZW21; Q9FJ49: PYL12; NbExp=3; IntAct=EBI-4441103, EBI-2363244;
CC Q9ZW21; Q9SN51: PYL13; NbExp=3; IntAct=EBI-4441103, EBI-25515027;
CC Q9ZW21; Q9SSM7: PYL3; NbExp=3; IntAct=EBI-4441103, EBI-2363144;
CC Q9ZW21; O80920: PYL4; NbExp=3; IntAct=EBI-4441103, EBI-2349683;
CC Q9ZW21; Q9FLB1: PYL5; NbExp=3; IntAct=EBI-4441103, EBI-2363181;
CC Q9ZW21; Q8S8E3: PYL6; NbExp=3; IntAct=EBI-4441103, EBI-2363192;
CC Q9ZW21; Q1ECF1: PYL7; NbExp=5; IntAct=EBI-4441103, EBI-2363203;
CC Q9ZW21; Q9FGM1: PYL8; NbExp=7; IntAct=EBI-4441103, EBI-2429535;
CC Q9ZW21; Q84MC7: PYL9; NbExp=8; IntAct=EBI-4441103, EBI-2349513;
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
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DR EMBL; AC004561; AAC95200.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08244.1; -; Genomic_DNA.
DR EMBL; DQ056553; AAY78704.1; -; mRNA.
DR EMBL; BT022047; AAY25459.1; -; mRNA.
DR EMBL; BT023483; AAY57322.1; -; mRNA.
DR PIR; F84695; F84695.
DR RefSeq; NP_180499.1; NM_128492.3.
DR AlphaFoldDB; Q9ZW21; -.
DR SMR; Q9ZW21; -.
DR BioGRID; 2837; 15.
DR DIP; DIP-48991N; -.
DR IntAct; Q9ZW21; 18.
DR STRING; 3702.AT2G29380.1; -.
DR PaxDb; Q9ZW21; -.
DR PRIDE; Q9ZW21; -.
DR EnsemblPlants; AT2G29380.1; AT2G29380.1; AT2G29380.
DR GeneID; 817487; -.
DR Gramene; AT2G29380.1; AT2G29380.1; AT2G29380.
DR KEGG; ath:AT2G29380; -.
DR Araport; AT2G29380; -.
DR TAIR; locus:2043142; AT2G29380.
DR eggNOG; KOG0698; Eukaryota.
DR HOGENOM; CLU_013173_20_0_1; -.
DR InParanoid; Q9ZW21; -.
DR OMA; WKNAMER; -.
DR OrthoDB; 1044139at2759; -.
DR PhylomeDB; Q9ZW21; -.
DR PRO; PR:Q9ZW21; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZW21; baseline and differential.
DR Genevisible; Q9ZW21; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR GO; GO:0035970; P:peptidyl-threonine dephosphorylation; IBA:GO_Central.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 3.60.40.10; -; 1.
DR InterPro; IPR000222; PP2C_BS.
DR InterPro; IPR036457; PPM-type_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase_dom.
DR Pfam; PF00481; PP2C; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; SSF81606; 1.
DR PROSITE; PS01032; PPM_1; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW Reference proteome.
FT CHAIN 1..362
FT /note="Probable protein phosphatase 2C 24"
FT /id="PRO_0000367953"
FT DOMAIN 77..360
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT BINDING 117
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 117
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 118
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 295
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 351
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 362 AA; 40297 MW; D127B5057DA3E0D4 CRC64;
MAEICYEVVT DACPSSVYES TPAHSRRRPR FQTVMHEDWE KNCKRSKQEA LATRYSSIPR
SSREDFSDQN VDVSSPRYGV SSVCGRRREM EDAVAIHPSF SSPKNSEFPQ HYFGVYDGHG
CSHVAARCRE RLHKLVQEEL SSDMEDEEEW KTTMERSFTR MDKEVVSWGD SVVTANCKCD
LQTPACDSVG STAVVSVITP DKIVVANCGD SRAVLCRNGK PVPLSTDHKP DRPDELDRIE
GAGGRVIYWD CPRVLGVLAM SRAIGDNYLK PYVSCEPEVT ITDRRDDDCL ILASDGLWDV
VSNETACSVA RMCLRGGGRR QDNEDPAISD KACTEASVLL TKLALARNSS DNVSVVVIDL
RR
