P2C34_ORYSI
ID P2C34_ORYSI Reviewed; 380 AA.
AC Q5MFV5; A2XM81; Q5MFV4;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Probable protein phosphatase 2C 34;
DE Short=OsPP2C34;
DE EC=3.1.3.16;
DE AltName: Full=BTH-induced protein phosphatase 2C 2;
DE Short=OsBIPP2C2;
GN Name=BIPP2C2; ORFNames=OsI_013174;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), AND INDUCTION.
RC STRAIN=cv. Yuanfengzao; TISSUE=Seedling;
RX PubMed=16436304; DOI=10.1016/j.jplph.2005.11.004;
RA Cao Y., Song F., Goodman R.M., Zheng Z.;
RT "Molecular characterization of four rice genes encoding ethylene-responsive
RT transcriptional factors and their expressions in response to biotic and
RT abiotic stress.";
RL J. Plant Physiol. 163:1167-1178(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT Arabidopsis.";
RL BMC Genomics 9:550-550(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5MFV5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5MFV5-2; Sequence=VSP_036261, VSP_036262;
CC Name=3;
CC IsoId=Q5MFV5-3; Sequence=VSP_036259, VSP_036260;
CC -!- INDUCTION: By benzothiadiazole (BTH). {ECO:0000269|PubMed:16436304}.
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
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DR EMBL; AY831390; AAW29521.1; -; mRNA.
DR EMBL; AY831391; AAW29522.1; -; mRNA.
DR EMBL; CM000128; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q5MFV5; -.
DR SMR; Q5MFV5; -.
DR STRING; 39946.Q5MFV5; -.
DR EnsemblPlants; BGIOSGA009773-TA; BGIOSGA009773-PA; BGIOSGA009773. [Q5MFV5-1]
DR Gramene; BGIOSGA009773-TA; BGIOSGA009773-PA; BGIOSGA009773. [Q5MFV5-1]
DR HOGENOM; CLU_013173_2_2_1; -.
DR OMA; QVRQAWP; -.
DR Proteomes; UP000007015; Chromosome 3.
DR ExpressionAtlas; Q5MFV5; differential.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 3.60.40.10; -; 1.
DR InterPro; IPR000222; PP2C_BS.
DR InterPro; IPR036457; PPM-type_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase_dom.
DR Pfam; PF00481; PP2C; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; SSF81606; 1.
DR PROSITE; PS01032; PPM_1; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Magnesium; Manganese; Metal-binding;
KW Protein phosphatase; Reference proteome.
FT CHAIN 1..380
FT /note="Probable protein phosphatase 2C 34"
FT /id="PRO_0000363280"
FT DOMAIN 32..335
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT BINDING 66
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 66
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 67
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 267
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 326
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT VAR_SEQ 233..255
FT /note="VGPPIPLKRPALSAEPSIQVRKL -> MVSGSISVTTLRCRLSSRIQGRV
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16436304"
FT /id="VSP_036259"
FT VAR_SEQ 256..380
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16436304"
FT /id="VSP_036260"
FT VAR_SEQ 288..317
FT /note="TGIANRLVKAALKEATRKREVSFRDLKTIE -> TVSSDNIYSIRSFHHREY
FT IFPVNERGVLTQ (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036261"
FT VAR_SEQ 318..380
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036262"
FT CONFLICT 84
FT /note="H -> L (in Ref. 1; AAW29521/AAW29522)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="A -> T (in Ref. 1; AAW29521/AAW29522)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 380 AA; 41754 MW; A52FA669C224987F CRC64;
MLRAVARCCG HWPPGAAAAD GMLWQTELRP HAAGEFSMAA AQANLAMEDQ AQVLASPAAT
LVGVYDGHGG ADASRFLRSR LFPHVQRFEK EQGGMSTEVI RRAFGAAEEE FLQQVRQAWR
QRPKMAAVGS CCLLGAISGD TLYVANLGDS RAVLGRRVVG GGVAVAERLT DEHNAASEEV
RRELTALNPD DAQIVVHARG AWRVKGIIQV SRTIGDVYLK KQEYSMDPVF RNVGPPIPLK
RPALSAEPSI QVRKLKPNDL FLIFASDGLW EHLSDDAAVQ IVFKNPRTGI ANRLVKAALK
EATRKREVSF RDLKTIEKGV RRHFHDDISV IVVYLDRHRG RRHTRVVDSS SNCTNAPVDI
YSSNSGQSVE TLQAHRGSGW
