ASF2_YEAST
ID ASF2_YEAST Reviewed; 525 AA.
AC P32448; D6VRF7;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Anti-silencing protein 2;
GN Name=ASF2; OrderedLocusNames=YDL197C; ORFNames=D1219;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9290207;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1029::aid-yea160>3.0.co;2-1;
RA Le S., Davis C., Konopka J.B., Sternglanz R.;
RT "Two new S-phase-specific genes from Saccharomyces cerevisiae.";
RL Yeast 13:1029-1042(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8896272;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1065::aid-yea995>3.0.co;2-f;
RA Verhasselt P., Voet M., Mathys J., Volckaert G.;
RT "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast
RT chromosome IV reveals the location of five known genes and characterizes at
RT least six new open reading frames including putative genes for ribosomal
RT protein L35 and a sugar transport protein.";
RL Yeast 12:1065-1070(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-525.
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9046097;
RX DOI=10.1002/(sici)1097-0061(199702)13:2<163::aid-yea54>3.0.co;2-4;
RA Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U.,
RA Schmidt E.R.;
RT "The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new
RT open reading frames, nine known genes and one gene for Gly-tRNA.";
RL Yeast 13:163-169(1997).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Derepression of silent mating type loci when overexpressed.
CC -!- MISCELLANEOUS: Present with 1910 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; L07649; AAC37513.1; -; Genomic_DNA.
DR EMBL; X83276; CAA58250.1; -; Genomic_DNA.
DR EMBL; Z74245; CAA98774.1; -; Genomic_DNA.
DR EMBL; X99000; CAA67472.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11667.1; -; Genomic_DNA.
DR PIR; S30767; S30767.
DR RefSeq; NP_010084.1; NM_001180257.1.
DR AlphaFoldDB; P32448; -.
DR SMR; P32448; -.
DR BioGRID; 31849; 56.
DR IntAct; P32448; 5.
DR MINT; P32448; -.
DR STRING; 4932.YDL197C; -.
DR iPTMnet; P32448; -.
DR PaxDb; P32448; -.
DR PRIDE; P32448; -.
DR EnsemblFungi; YDL197C_mRNA; YDL197C; YDL197C.
DR GeneID; 851330; -.
DR KEGG; sce:YDL197C; -.
DR SGD; S000002356; ASF2.
DR VEuPathDB; FungiDB:YDL197C; -.
DR eggNOG; ENOG502T1NR; Eukaryota.
DR HOGENOM; CLU_518903_0_0_1; -.
DR InParanoid; P32448; -.
DR OMA; PHIANLK; -.
DR BioCyc; YEAST:G3O-29581-MON; -.
DR PRO; PR:P32448; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P32448; protein.
DR GO; GO:0000781; C:chromosome, telomeric region; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0030466; P:silent mating-type cassette heterochromatin assembly; IMP:SGD.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..525
FT /note="Anti-silencing protein 2"
FT /id="PRO_0000064696"
FT REGION 467..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 467..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 482..497
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 500..514
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 525 AA; 59347 MW; 8018D3FFD908A37C CRC64;
MPKNRGVLDA ITRSVIDGSD QESSSSFNSD KEYAAVTKGL SSSRVQKKSS LRQMKSKVKE
LQSLVNHYRE NEAALVSSAK LLSGEIIGYE IKMASLHGKM KSILDENNAL KETHKSSAEK
RIELVRLPSS KEERNYDEYT LLVNLKKEIC AKLQDYKNVQ NTVNTKLDEI HTFHEKYYEG
LELSLDSKVF DAESSKELAK VRRELNNVRK NSEIKVNNLK MQLLQATKSL EHLKKQAKAK
DDYLKCIPEL VDKANLTMLS YKKSIANQRE TIEALQAELS QQSETKGQIE TETQNQVQIP
TNVTLVDPFE ENNPEDLFAI QEQELQDLRL HKKMADERSR TTHLHLERKN NTIKLLQSYV
QSLIQRLPPA QRKHHLGIFQ KLGSEKSCPL APAVASTYAP LLLLSQHSNH QEIDNTPQRL
LLAAPDGQSY SEKSTTLNLD YSSRKSYLSR LQPPHIANLK SLTLKTLPRV PTDSPQLPSK
DKSQETAKKD DRPKLVANEP VTLDTSTPPV AQSLADSKHC SGLHK
